Hoefer TE 22, User Manual, Page: 14 / 34

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Operating instructions
Perform the transfer as soon as possible after
electrophoresis to minimize band diffusion. Each
step is described below.
Prepare the buffer
Prepare a minimum of 1.5 liters of the appropri-
ate transfer buffer. Chill the buffer before use if
possible.
Prepare the unit
1
Rinse the transfer tank and cassettes with
distilled water.
2
Active cooling is optional but strongly recommended. If
no active cooling will be used, go to step 3.
Note: Connect the heat exchanger to a circulator
bath such as the MultiTemp ™ III.
Circulate only water or 50/50 water/ethylene glycol
to prevent damage to the unit.
The circulator pump must not generate a pressure
greater than 0.7 bar (10 psi) above atmospheric
pressure.
Set the temperature to 10 °C or higher if circulat-
ing only water. If using 50/50 ethylene glycol/
water, the temperature can be set lower.
Start the circulator bath at the same time as
the transfer.
First attach tubing to the red pressure relief valve
between the water inlet and outlet ports and insert
the free end into the bath or other container or drain
to catch any pressure relief overflow. The relief valve
opens if the pressure within the heat exchanger
exceeds 10 psi.
Prepare two lengths of 9 mm (3/8”) vinyl or silicone
tubing. Slide hose clamps (4 total) onto each end of
two lengths of tubing. Attach one end of each length
of tubing to a heat exchanger port. Attach the free
Note: For quick and easy connec-
tions, install Quick-fit coupler
fittings with valves in the line.
Note: Refer to the Electrotransfer
Notes section for a discussion of
membranes and buffers.