Hoefer SE 260, User Manual, Page: 24 / 32

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p20
Final concentrations
separating gel stacking gel electrophoresis
buffer
Acrylamide conc. 10% T*, 2.6% C 4% T, 2.6% C
Tris-Cl 0.375 M 0.125 M
Tris-glycine 0.025 M Tris base
0.192 M glycine
pH 8.8 6.8 ~8.3
SDS 0.1% 0.1% 0.1%
Ammonium persulfate (APS) 0.05% w/v 0.05–0.1% w/v
TEMED † 0.05% v/v 0.05–0.1% v/v
* To achieve any other desired final concentration, adjust the acrylamide stock and water volumes.
†Tetramethylethylenediamine