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15
U n z i p p i n g G e n e s
1
MBIN008
Insta NX® Fungal DNA Purification Kit
Kit Contents
Product Code
Reagents provided
12 Preps
DS0401
Fungal DNA Extraction Cartridges
12 nos.
DS0337
1 ml tip set
12 nos.
DS0016
Lysis Buffer (PL)
6 ml
DS0017
Precipitation Buffer (PS)
1.8 ml
DS0003
RNase A Solution (20 mg/ml)
300 µl
DS0040
Elution Buffer (ET) [10 mM Tris-Cl, pH 8.5]
350 µl
DBCA04
HiBead Tubes
12 nos.
DSCA02
HiShredder (in DBCA016 Collection Tube)
12 nos.
PW1139
Collection Tubes, Polypropylene (2.0 ml)
40 nos.
DBCA021
Columns for Insta NX
12 nos.
Introduction
This kit provides a fast and easy method to purify total DNA from fungal samples for
downstream applications such as PCR, Southern blotting technique etc. The DNA purification
procedure using the column comprises of three steps viz. adsorption of DNA to the Super- S
membrane, removal of residual contaminants and elution of pure genomic DNA. The Super- S
columns have a high binding capacity and high quality genomic DNA is obtained from various
species.
Insta NX® Fungal DNA Purification
Kit
This kit simplifies isolation of DNA from various fungal samples with vacuum based innovative
Super- S column technology. The Super- S columns contains specially developed membranes for
optimal binding of genomic DNA. After the initial binding of DNA, impurities like proteins,
polysaccharides, low molecular weight metabolites and salts are removed by short washing
steps. High quality DNA is finally eluted in the Elution Buffer provided with the kit.
The salient features of this kit are as follows:
a. Cross- Contamination free
b. 12 samples can be processed at a time.
c. Prefilled reagent cartridge
d. High purity, high yield, as good as manual.
Elution
The yield of genomic DNA depends on the sample type and the number of cells in the sample.
An elution with 100 µl of Elution Buffer (ET) will provide sufficient DNA to carry out multiple
amplification reactions. Elution with volume less than 100 µl will increase the final DNA
concentration, but will reduce the overall DNA yield. The eluted DNA ranges in size upto 20-30
kb, and is suitable for direct use in PCR, restriction digestion and Southern blotting applications.
