TECHNICAL INFORMATION
Membrane
Material
Polyethersulfone (PES)
Molecular Cut-Off
500kDa, 2MDa
Outer Diameter
0.28 mm
Length
1, 2, 3 and 4 mm
Probe Shaft
Material
Stainless-steel
Diameter
0.40 mm
Length
14 mm
Internal Volume
Inlet Volume
0.1 µL
Outlet Volume
1 µL
CMA 11
55
kDa
&
2MDa
Microdialysis Probe User’s Manual
0.40
0.2
8
shaft
steel shaft
outlet
intlet
membrane
Instructions for CMA
11 5
00
kDa
& 2MDa
Microdialysis Probe
s
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
Note: The 500 kDa
and 2 MDa
Cut Off membrane has to be use in a Push-Pull system to avoid loss of fluid out to the
surrounding tissue (ultra-filtration). Here the use of a CMA syringe pump and REGLO ICC Pump is recommended.
Load the peristaltic pump with the "FEP Tubing Connector Peristaltic
Kit
". Adjust the lengths of the FEP tubing if needed for a
lower dead volume; recalculate the outlet volume if needed (1.2 µl/100 mm length)
Run the pump to make sure that liquid leaves the tip of the syringe cannula.
Fill a microsyringe with perfusion fluid and mount it in the CMA Syringe Pump. The Perfusion Fluid must be clean, at
room temperature and preferably degassed.
Place the inlet end of the “FEP Tubing Connector Peristaltic
Kit
” in a beaker with Perfusion Fluid and flush the pump to fill all
tubing with perfusion fluid. Make sure there is no air bubbles in the complete tubing set.
S
et the pump to the required perfusion flow, usually 1 – 5 µL/min.
Prepare a desired length of tubing with a Tubing Adapter on both ends and connect the tubing to the inlet of the probe.
Remove the protection tube from the probe carefully. Short cannula = inlet, long cannula = outlet. Tubing Adapters and FEP
tubing should be used for all connections. T
o facilitate the handling of Tubing Adapters, they should be soaked in Ethanol
70% for minimum 10 minutes.
Mount the probe in a Probe/Guide Clip on the CMA 130 In Vitro Stand. Put the probe membrane into a vial filled with
perfusion fluid. Connect the inlet tubing of the probe to the syringe cannula by sliding the Tubing Adapter over the cannula.
Flush the probe with perfusion fluid at 8-10µL/min for 4-5 min to wash out air. Knock on the shaft of the clip to help the air to
flush away. At this process the Ultra High Cut Off membrane will look as leaking but this is due to ultra-filtration of fluid
through the membrane.
Set the pump to the required perfusion flow, usually 1 – 5 µL/min. Make sure both pumps have the same flow rate.
Connect the inlet “FEP Tubing Connector Peristaltic
Kit
” at the peristaltic pump to the outlet of the probe
11
.
12
.
13
.
Lift up the probe from the vial and check that the membrane doesn’t ultrafiltrate or dry out; it should look filled out but
not sweating. Lower the probe into the vial and control the outlet volume for the complete system.
The system with the probe is now ready for use.
When changing sample vials, remember to consider the internal volume in the system (see TECHNICAL INFORMATION).
This causes a delay that must be calculated when using low perfusion rates and short sampling times
