Hanna Instruments HI83303 Instruction Manual Download

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INSTRUCTION MANUAL

HI83303

Aquaculture Photometer

Summary of Contents for HI83303

Page 1: ...INSTRUCTION MANUAL HI83303 Aquaculture Photometer...

Page 2: ...the instrument as well as a precise idea of its versatility If you need additional technical information do not hesitate to e mail us at tech hannainst com or view our worldwide contact list at www ha...

Page 3: ...SAMPLE USER NAMES TO LOG DATA 5 7 DATA MANAGEMENT 5 8 CONTEXTUAL HELP 6 PHOTOMETER MODE 6 1 METHOD SELECTION 6 2 COLLECTING AND MEASURING SAMPLES AND REAGENTS 6 2 1 PROPER USE OF SYRINGE 6 2 2 PROPER...

Page 4: ...COPPER LOW RANGE 8 11 COPPER HIGH RANGE 8 12 NITRATE 8 13 NITRITE MARINE ULTRA LOW RANGE 8 14 NITRITE LOW RANGE 8 15 NITRITE HIGH RANGE 8 16 OXYGEN DISSOLVED 8 17 pH 8 18 PHOSPHATE MARINE ULTRA LOW RA...

Page 5: ...y damaged or defective item must be returned in its original packing material with the supplied accessories 2 SAFETY MEASURES The chemicals contained in the reagent kits may be hazardous if improperly...

Page 6: ...4 01 6 86 7 01 9 18 10 01 pH Electrode Intelligent pH temperature electrode Temperature Range 20 0 to 120 0 C 4 0 to 248 0 F Resolution 0 1 C 0 1 F Accuracy 0 5 C 0 9 F 25 C 77 F Additional Specifica...

Page 7: ...to confirm meter functionality Dual purpose micro USB flash drive Li polymer rechargeable battery Auto off Absorbance mode User and sample name entry GLP features 4 2 PRECISION AND ACCURACY Precision...

Page 8: ...ective port covers 5 Light blocking cover panel 6 Cuvette holder 7 ON OFF power button 8 3 5 mm TRRS jack input for digital electrodes 9 Standard USB host connector for data transfer to a USB flash dr...

Page 9: ...ss second level functions Press to move right in a menu or to increment a set value Press to access the setup screen Press to log the current reading Press to review saved logs Press to exit the curre...

Page 10: ...tput Improved optical filters ensure greater wavelength accuracy and allow a brighter stronger signal to be received The end result is higher measurement stability and less wavelength error A focusing...

Page 11: ...charged meter connected to AC DC adapter battery capacity no external adapter battery near 0 no external adapter battery at 0 no external adapter To conserve battery the meter will turn off automatic...

Page 12: ...value Press the Accept key to confirm the value or ESC to return to the Setup menu without saving the new value Date Time Press the Modify key to change the date time Press the functional keys or the...

Page 13: ...language Press Select to choose one of the 7 languages installed Beeper Option Enable or Disable When enabled a short beep is heard every time a key is pressed A long beep alert sounds when the presse...

Page 14: ...5 mm port marked with EXT PROBE located at the rear of the meter If the meter is in Photometer Mode set the meter to Probe Mode by pressing the MODE key 5 4 MODE SELECTION The HI83303 has two operatio...

Page 15: ...O LOG DATA A sample ID and user ID can be added to the saved log Use the keys to highlight the Sample ID or User ID then press Modify Text Entry Sample ID and User ID care entered using the alphanumer...

Page 16: ...a can be exported to a USB flash drive or to a PC To access Data Export functions press Recall then Export Use the keys to select the desired export location For export to USB Flash Drive insert the U...

Page 17: ...n Log data is exported as a single file containing all logged photometer and probe data The file name is HI83303 csv The CSV file Comma Separated Values may be opened with a text editor or spreadsheet...

Page 18: ...p of the syringe Then keeping the syringe in vertical position above the cuvette push the plunger down into the syringe the desired volume has been delivered into the cuvette 6 2 2 PROPER USE OF DROPP...

Page 19: ...ing technique is indicated with invert to mix and the following icon b Shaking the cuvette moving the cuvette up and down The movement may be gentle or vigorous This mixing method is indicated with sh...

Page 20: ...hod requires a different preparation procedure reaction times sample preparations etc If a timer or timers are necessary for proper sample preparation the Timer key will be available To use a reaction...

Page 21: ...8 to 25 C 64 5 to 77 0 F Validation of the HI83303 involves absorbance measurements of certified HANNA CAL Check Standards see Accessories The CAL Check screen guides the user through the measurement...

Page 22: ...pressing the MODE key 2 Press the METHOD key 3 Highlight the appropriate Absorbance method according to the wavelength to be used then press Select Absorbance methods are located at the bottom of the...

Page 23: ...the buffer number Buffer 1 for the 1st buffer Buffer 2 for the 2nd buffer The following functions are available in pH Calibration Mode Clear Press to clear the current calibration from the probe Conf...

Page 24: ...asurement screen press the Calibrate key to begin the calibration process When the reading is stable and close to the selected buffer the Confirm key will become available Press Confirm to accept and...

Page 25: ...ect buffer selection Cleaning may also improve this response Wrong Temperature The buffer temperature is too extreme for the selected buffer value 7 3 pH MEASUREMENT The HI83303 can be used to perform...

Page 26: ...rinse the electrode with a small amount of sample Discard the rinse Submerse the electrode tip approximately 3 cm 1 into the sample to be tested and stir the sample gently Make sure the electrode jun...

Page 27: ...patible Calibration The probe s current calibration is not compatible with this meter The calibration must be cleared to use this probe Exceeded Probe Range The pH and or temperature measurement excee...

Page 28: ...calibrations and measurements To view the GLP information press the GLP key from the Probe Measurement screen The pH GLP screen displays the following information about the last pH calibration Date an...

Page 29: ...k the electrode in HI70300 or HI80300 storage solution for at least one hour For refillable electrodes If the filling solution electrolyte is more than 2 cm 1 below the fill hole add HI7082 or HI8082...

Page 30: ...double junction electrodes Allow the electrode to stand upright for 1 hour Follow the Storage Procedure above Cleaning Procedure Use diagnostic messages to aid pH electrode troubleshooting Several cl...

Page 31: ...linity Reagent 1 mL HI93755 53 Chlorine Removal Reagent 1 drop REAGENT SETS HI775 26 Reagents for 25 tests For other accessories see page 87 MEASUREMENT PROCEDURE Select the Alkalinity method using th...

Page 32: ...add one drop of HI93755 53 Chlorine Removal Reagent to the unreacted sample Add 1mL of HI775S Alkalinity Reagent to the sample using a 1 mL syringe Replace the cap and invert 5 times Reinsert the cuve...

Page 33: ...ity HI755S Alkalinity Reagent 1 mL REAGENT SETS HI755 26 Reagents for 25 tests For other accessories see page 87 MEASUREMENT PROCEDURE Select the Alkalinity Marine method using the procedure described...

Page 34: ...gently invert 5 times Reinsert the cuvette into the instrument and close the lid Press Read to start the reading The instrument displays the results in mg L as calcium carbonate CaCO3 Press or to acce...

Page 35: ...Low Range Reagent A 4 drops HI93700B 0 Ammonia Low Range Reagent B 4 drops REAGENT SETS HI93700 01 Reagents for 100 tests HI93700 03 Reagents for 300 tests For other accessories see page 87 MEASUREME...

Page 36: ...nd mix the solution Reinsert the cuvette into the instrument and close the lid Press Timer and the display will show the countdown prior to the measurement or alternatively wait for 3 minutes and 30 s...

Page 37: ...rm key to convert the result to mg L of ammonia NH3 and ammonium NH4 Press or to return to the measurement screen INTERFERENCE Interference may be caused by Acetone Alcohols Aldehydes Glycine Hardness...

Page 38: ...um Range Reagent A 4 drops HI93715B 0 Ammonia Medium Range Reagent B 4 drops REAGENT SETS HI93715 01 Reagents for 100 tests HI93715 03 Reagents for 300 tests For other accessories see page 87 MEASUREM...

Page 39: ...the cap and mix the solution Reinsert the cuvette into the instrument and close the lid Press Timer and the display will show the countdown prior to the measurement or alternatively wait for 3 minutes...

Page 40: ...result in mg L of ammonia NH3 and ammonium NH4 Press or to return to the measurement screen INTERFERENCES Interference may be caused by Acetone Alcohols Aldehydes Glycine Hardness above 1 g L Iron Org...

Page 41: ...High Range Reagent A 4 drops HI93733B 0 Ammonia High Range Reagent B 9 mL REAGENT SETS HI93733 01 Reagents for 100 tests HI93733 03 Reagents for 300 tests For other accessories see page 87 MEASUREMEN...

Page 42: ...solution Reinsert the cuvette into the instrument and close the lid Press Timer and the display will show the countdown prior to the measurement or alternatively wait for 3 minutes and 30 seconds and...

Page 43: ...esult to mg L of ammonia NH3 and ammonium NH4 Press or to return to the measurement screen INTERFERENCES Interference may be caused by Acetone Alcohols Aldehydes Glycine Hardness above 1 g L Iron Orga...

Page 44: ...2A Ca Calcium Reagent A 7 mL HI93752B Ca Calcium Reagent B 1 mL REAGENT SETS HI937521 01 Reagents for 50 tests HI937521 03 Reagents for 150 tests For other accessories see page 87 MEASUREMENT PROCEDUR...

Page 45: ...e meter is zeroed and ready for measurement Remove the cuvette Add 1 mL of HI93752B Ca Calcium Reagent B to the sample using the 1 mL syringe Invert the cuvette 10 times to mix about 15 seconds Reinse...

Page 46: ...the cuvette into the instrument and close the lid Press Read to start the reading The instrument displays the results in mg L of calcium Ca2 INTERFERENCES Interferences may be caused by Acidity as Ca...

Page 47: ...Calcium Reagent B 1 packet REAGENT SETS HI758 26 Reagents for 25 tests For other accessories see page 87 MEASUREMENT PROCEDURE Select the Calcium Marine method using the procedure described in the Met...

Page 48: ...e Add one packet of HI7582 Calcium Reagent B Replace the cap and shake vigorously for 15 seconds or until the powder is completely dissolved Allow air bubbles to dissipate for 15 seconds before taking...

Page 49: ...ode Description Quantity HI93701A F Free Chlorine Reagent A 3 drops HI93701B F Free Chlorine Reagent B 3 drops REAGENT SETS HI93701 F Reagents for 300 tests liquid HI93701 01 Reagents for 100 tests po...

Page 50: ...insert the cuvette into the instrument and close the lid Press Timer and the display will show the countdown prior to the measurement or alternatively wait for 1 minute and press Read When the timer e...

Page 51: ...ine Ozone Oxidized forms of Chromium and Manganese In case of water with hardness greater than 500 mg L CaCO3 shake the sample for approximately 2 minutes after adding the powder reagent If the water...

Page 52: ...Chlorine Reagent A 3 drops HI93701B T Total Chlorine Reagent B 3 drops HI93701C T Total Chlorine Reagent C 1 drop REAGENT SETS HI93701 T Reagents for 300 tests liquid HI93711 01 Reagents for 100 total...

Page 53: ...ent and close the lid Press Timer and the display will show the countdown prior to the measurement or alternatively wait for 2 minutes and 30 seconds and press Read When the timer ends the meter will...

Page 54: ...elated procedure if both values are desired INTERFERENCES Interference may be caused by Bromine Iodine Ozone Oxidized forms of Chromium and Manganese In case of water with hardness greater than 500 mg...

Page 55: ...per Low Range Reagent 1 packet REAGENT SETS HI95747 01 Reagents for 100 tests HI95747 03 Reagents for 300 tests For other accessories see page 87 MEASUREMENT PROCEDURE Select the Copper LR method usin...

Page 56: ...Press Timer and the display will show the countdown prior to the measurement or alternatively wait for 45 seconds and press Read When the timer ends the meter will perform the reading The instrument d...

Page 57: ...r High Range Reagent 1 packet REAGENT SETS HI93702 01 Reagents for 100 tests HI93702 03 Reagents for 300 tests For other accessories see page 87 MEASUREMENT PROCEDURE Select the Copper HR method using...

Page 58: ...Press Timer and the display will show the countdown prior to the measurement or alternatively wait for 45 seconds and press Read When the timer ends the meter will perform the reading The instrument...

Page 59: ...ate Reagent 1 packet REAGENT SETS HI93728 01 Reagents for 100 tests HI93728 03 Reagents for 300 tests For other accessories see page 87 MEASUREMENT PROCEDURE Select the Nitrate method using the proced...

Page 60: ...tively affect the measurement Note The method is technique sensitive See procedure on page 20 Cuvette Preparation for proper mixing technique Reinsert the cuvette into the instrument and close the lid...

Page 61: ...NO3 Press or to return to the measurement screen INTERFERENCES Interference may be caused by Ammonia and amines as urea and primary aliphatic amines Chloride above 100 ppm Chlorine above 2 ppm Copper...

Page 62: ...or 25 tests For other accessories see page 87 MEASUREMENT PROCEDURE Select the Nitrite Marine ULR method using the procedure described in the Method Selection section see page 17 Fill the cuvette with...

Page 63: ...d level functions Press the Chem Frm key to convert the result to g L of nitrite NO2 and sodium nitrite NaNO2 Press or to return to the measurement screen INTERFERENCES Interference may be caused by t...

Page 64: ...REAGENT SETS HI93707 01 Reagents for 100 tests HI93707 03 Reagents for 300 tests For other accessories see page 87 MEASUREMENT PROCEDURE Select the Nitrite LR method using the procedure described in t...

Page 65: ...ay will show the countdown prior to the measurement or alternatively wait for 15 minutes and press Read When the timer ends the meter will perform the reading The instrument displays concentration in...

Page 66: ...rcurous silver antimonious bismuth auric lead metavanadate and chloroplatinate Strongly reducing and oxidizing reagents High levels of nitrate above 100 mg L could yield falsely high readings due to a...

Page 67: ...eagent 1 packet REAGENT SETS HI93708 01 Reagents for 100 tests HI93708 03 Reagents for 300 tests For other accessories see page 87 MEASUREMENT PROCEDURE Select the Nitrite HR method using the procedur...

Page 68: ...splay will show the countdown prior to the measurement or alternatively wait for 10 minutes and press Read When the timer ends the meter will perform the reading The instrument displays concentration...

Page 69: ...nts for 100 tests HI93732 03 Reagents for 300 tests For other accessories see page 87 MEASUREMENT PROCEDURE Select the Oxygen dissolved method using the procedure described in the Method Selection sec...

Page 70: ...ating agent dissolves completely The sample is ready for measurement when it is yellow and completely clear Fill the first cuvette 1 with 10 mL of the unreacted sample up to the mark and replace the c...

Page 71: ...ert the cuvette into the holder and close the lid Press Read to start the reading The instrument will display the results in mg L of oxygen O2 INTERFERENCES Interferences may be caused by reducing and...

Page 72: ...SETS HI 93710 01 Reagents for 100 pH tests HI 93710 03 Reagents for 300 pH tests For other accessories see page 87 MEASUREMENT PROCEDURE Select the pH method using the procedure described in the Meth...

Page 73: ...nd add 5 drops of HI93710 0 pH Reagent Indicator Replace the cap and mix the solution Reinsert the cuvette into the instrument and close the lid Press the Read key to start the reading The instrument...

Page 74: ...For other accessories see page 87 MEASUREMENT PROCEDURE Select the Phosphate Marine ULR method using the procedure described in the Method Selection section see page 17 Rinse cap and shake the cuvett...

Page 75: ...eading The instrument displays concentration in g L of phosphorus P Press or to access the second level functions Press the Chem Frm key to convert the result to g L of phosphate PO4 3 and phosphorus...

Page 76: ...e page 87 MEASUREMENT PROCEDURE Select the Phosphate LR method using the procedure described in the Method Selection section see page 17 Rinse cap and shake the cuvette several times with unreacted sa...

Page 77: ...he instrument displays concentration in mg L of phosphate PO4 3 Press or to access the second level functions Press the Chem Frm key to convert the result to mg L of phosphorus P and phosphorus pentox...

Page 78: ...drops HI93717B 0 Phosphate High Range Reagent B 1 packet REAGENT SETS HI93717 01 Reagents for 100 tests HI93717 03 Reagents for 300 tests For other accessories see page 87 MEASUREMENT PROCEDURE Selec...

Page 79: ...nd the display will show the countdown prior to the measurement or alternatively wait for 5 minutes and press Read When the timer ends the meter will perform the reading The instrument displays the re...

Page 80: ...ease check that the sample does not contain any debris Light High There is too much light to perform a measurement Please check the preparation of the zero cuvette 9 ERROR DESCRIPTIONS Ambient tempera...

Page 81: ...to 5 00 mg L DPD Chlorine Total LR 0 00 to 5 00 mg L DPD Copper LR 0 000 to 1 500 mg L Bicinchoninate Copper HR 0 00 to 5 00 mg L Bicinchoninate Nitrate 0 0 to 30 0 mg L Cadmium reduction Nitrite Mari...

Page 82: ...free tests powder HI93701 F 300 chlorine free tests liquid HI93701 T 300 chlorine total tests liquid HI93702 01 100 copper HR tests HI93702 03 300 copper HR tests HI93707 01 100 nitrite LR tests HI937...

Page 83: ...728 03 300 nitrate tests HI93732 01 100 dissolved oxygen tests HI93732 03 300 dissolved oxygen tests HI93733 01 100 ammonia HR tests HI93733 03 300 ammonia HR tests HI937521 01 50 calcium fresh water...

Page 84: ...el filled non refillable pH temperature electrode HI12301 Plastic body double junction gel filled non refillable pH temperature electrode with enhanced diagnostics HI10480 Glass body double junction w...

Page 85: ...00 mL HI7009L pH 9 18 Buffer Solution 500 mL HI7010L pH 10 01 Buffer Solution 500 mL HI8004L pH 4 01 Buffer Solution in FDA approved bottle 500 mL HI8006L pH 6 86 Buffer Solution in FDA approved bottl...

Page 86: ...ning Solution 500 mL HI8061L General Cleaning Solution in FDA approved bottle 500 mL HI8073L Protein Cleaning Solution in FDA approved bottle 500 mL HI8077L Oil Fat Cleaning Solution in FDA approved b...

Page 87: ...le and stopper HI740142P 1 mL graduated syringe 10 pcs HI740143 1 mL graduated syringe 6 pcs HI740144 pipette tip 6 pcs HI740157P plastic refilling pipette 20 pcs HI740220 25 mL graduated glass vial 2...

Page 88: ...arbon for 50 tests demineralizer bottle for 10 L of water 100 mL graduated beaker with cap 170 mL graduated beaker with cap 3 mL pipette 60 mL syringe 5 mL syringe graduated cylinder spoon funnel filt...

Page 89: ...t g L micrograms per liter ppb mg L milligrams per liter ppm g L grams per liter ppt mL milliliter GLP good laboratory practice UHR ultra high range ULR ultra low range HR high range MR medium range L...

Page 90: ...nterferences to other electronic equipment Take all necessary steps to correct such interferences Any variation introduced by the user to the supplied equipment may degrade the instruments EMC perform...

Page 91: ...of purchase serial number and the nature of the problem If the repair is not covered by the warranty you will be notified of the charges incurred If the instrument is to be returnedtoHannaInstruments...

Page 92: ...Hanna Instruments Inc Highland Industrial Park 584 Park East Drive Woonsocket RI 02895 USA www hannainst com Local Office Hanna Instruments USA 270 George Washington Highway Smithfield RI 02917 Phone...

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