background image

7

The °Baumé scale is based on density and was originally designed to mea‑
sure the mass of sodium chloride in water. °Baumé is used in wine making 
to measure the sugar in must. The 

HI96812

 converts the %Brix reading 

to °Baumé based on the table found in the Official Methods of Analysis 
of AOAC International, 18

th

 Edition. 1 °Baumé is approximately equal to  

1.8 %Brix, and 1 °Baumé is roughly equivalent to 1 % alcohol when the 
wine is fully fermented.

In the 

HI96812

, light from an LED passes through a prism in contact with 

the sample. An image sensor determines the critical angle at which the light 
is no longer refracted through the sample.

The °Baumé determination is made by measuring the refractive index of a 
solution. Refractive Index is an optical characteristic of a substance and the 
number of dissolved particles in it. Refractive Index is defined as the ratio of 
the speed of light in empty space to the speed of light in the substance. A 
result of this property is that light will “bend”, or change direction, when it 
travels through a substance of different refractive index. This is called refraction.
When passing from a material with a higher to lower refractive index, there 
is a critical angle at which an incoming beam of light can no longer refract, 
but will instead be reflected off the interface.  The critical angle can be used 
to easily calculate the refractive index according to the equation:

sin (

θ

critical

) = n

2

 / n

1

Where n

2

 is the refractive index of the lower‑density medium; n

1

 is the 

refractive index of the higher‑density medium.

PRINCIPLE OF OPERA

TION

Summary of Contents for HI 96812

Page 1: ...HI96812 Refractometer for Sucrose in Wine and Grape Products Measurements INSTRUCTION MANUAL...

Page 2: ...this instrument as well as a precise idea of its versatility If you need additional technical information do not hesitate to e mail us at tech hannainst com or view our worldwide contact list at www h...

Page 3: ...ons Principle of Operation Functional Description Display Elements Measurement Guidelines Calibration Procedure Measurement Procedure Making a Standard Brix Solution To Change Temperature Unit Battery...

Page 4: ...occurred contact your local Hanna Instruments Office Each HI96812 instrument is supplied with 9 V battery Instruction manual Plastic pipette Quality Certificate Note Save all packing material until y...

Page 5: ...ibration with deionized or distilled water Within seconds the instrument measures the refractive index of the grape These digital refractometers eliminate the uncertainty associated with mechanical re...

Page 6: ...een 10 0 and 40 0 C 50 0 104 0 F Measurement Time Approximately 1 5 seconds Minimum Sample Volume 100 L cover prism totally Light Source Yellow LED Sample Cell StainlessSteelringandflintglassprism Cas...

Page 7: ...the refractive index of a solution Refractive Index is an optical characteristic of a substance and the number of dissolved particles in it Refractive Index is defined as the ratio of the speed of li...

Page 8: ...1 Liquid Crystal Display LCD 2 Secondary Display 3 Primary Display 4 READ Key User Measurement 5 ZERO Key User Calibration 6 ON OFF 7 Stainless Steel Sample Well and Prism TOP VIEW 8 Battery Cover 9 B...

Page 9: ...g 4 SETUP Factory Calibration Tag 5 CAL Calibration Tag 6 Measurement Units 7 Automatic Temperature Compensation blinkswhentemperatureexceeds10 0 40 0 C 50 0 104 0 Frange 8 Temperature Units 9 Seconda...

Page 10: ...ganic solvents and extremely hot or cold solutions Particulate matter in a sample may scratch the prism Absorb sample with a soft tissue and rinse sample well with deionized or distilled water between...

Page 11: ...s or if environmental changes have occurred since the last calibration 2 Using a plastic pipette fill the sample well with distilled or deionized water Make sure the prism is completely covered Note I...

Page 12: ...the ZERO water standard with a soft tissue Use care not to scratch the prism surface Dry the surface completely The instrument is ready for sample measurement Note If the instrument is turned off the...

Page 13: ...e instrument is ready for the next sample Note The last measurement value will be displayed until the next sample is measured or the instrument is turned off Temperature will be continuously updated T...

Page 14: ...Brix g Sucrose g Water g Total 25 25 000 75 000 100 000 To make a Brix Solution follow the procedure below Place container such as a glass vial or dropper bottle that has a cover on an analytical bal...

Page 15: ...F key continuously for approximately 15 seconds The LCD will display the all segment screen followed by a screen with the model number on the primary display and the version number on the secondary di...

Page 16: ...e the instrument is off Turn instrument upside down and remove the battery cover by turning it counterclockwise Extract the battery from its location Replace with a new 9V battery making sure to obser...

Page 17: ...t LO primary display Cal segment ON Temperature exceeds ATC low limit 10 0 C during calibration t HI primary display Cal segment ON Temperature exceeds ATC high limit 40 0 C during calibration Air Pr...

Page 18: ...18 MAN96812 04 17 ACCESSORIES Code Description HI4020 11 50 Brix Standard 10 mL HI740157P Plastic refilling pipette 20 pcs HI740029P 9V Battery 10 pcs...

Page 19: ...mber and the nature of the problem If the repair is not covered by the warranty you will be notified of the charges incurred If the instrument is to be returned to Hanna Instruments first obtain a Ret...

Page 20: ...ents Inc Highland Industrial Park 584 Park East Drive Woonsocket RI 02895 USA www hannainst com Local Office Hanna Instruments USA 270 George Washington Highway Smithfield RI 02917 Phone 800 426 6287...

Reviews: