38
Blank
Sample
Blank
Digested
blank
Sample
Digested
sample
←
green label
→
Sample
Nitrogen, Total HR
•
Remove the cap from the vials and add the content of one packet of HI 93767-0 Total Nitrogen
Reagent to each vial. Replace the cap tightly and shake the vials gently for 15 seconds.
•
Press
Start
to activate the 2 minutes timer or wait for 2 minutes (without shaking the vials) to allow
the reaction to complete.
• Remove the cap from two Reagent Vials (
green label
).
• Add exactly 2.0 mL of digested sample (sample vial) to one Reagent Vial (sample vial), and 2.0 mL
of digested blank (blank vial) to the other vial (blank vial), while keeping the vials at a 45-degree
angle.
• Replace the cap tightly and invert the vials 10 times.
Warning
: The vials will become hot during mixing, be careful when
handling them.
Note
: The method is technique sensitive: to obtain most reproducible
results it is strongly recommended to follow carefully the “invert”
procedure described on page 10.
• Place the blank vial into the holder and push it completely down.
Blank
Sample
Blank