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19

CURRENT DATE SET

Press 

CAL

 when the current date is displayed. The year will start blinking.

Press the 

ARROW

 

keys to change the year.

Press 

RANGE

. The month will start blinking.

Press the 

ARROW

 

keys to change the month.

Press 

RANGE

. The day will start blinking.

Press the 

ARROW

 keys to change the day.

Press 

CFM

 to save the modified value.

Press 

CAL

 to escape without saving.

pH RESOLUTION SET

Press 

CAL

 when the pH range is displayed. The pH range (0.01 or 0.1) will

start blinking.

Press the 

ARROW

 

keys to change the pH range.

Press 

CFM

 to save the modified pH range.

Press 

CAL

 to escape without saving.

BEEP STATUS SET

Press 

CAL

 when the beep status is displayed. Beep status (On or OFF) will

start blinking.

DATE

DATE

DATE

pH

All manuals and user guides at all-guides.com

Summary of Contents for HI 250

Page 1: ...1 Instruction Manual HI 250 HI 251 Microprocessor Based pH mV ºC Bench Meters www hannainst com All manuals and user guides at all guides com a l l g u i d e s c o m ...

Page 2: ...tcovered If service is required contact the dealer from whom you purchased the instruments If under warranty report the model number date of purchase serial number and the nature of the problem If the repair is not covered by the warranty you will be notified of the charges incurred If the instruments are to be returned to Hanna Instruments first obtain a Returned Goods Authorization number from t...

Page 3: ... accessories The HANNA HI 250 and HI 251 are logging microprocessor based pH and temperature bench meters HI 251 can also measure Oxidation Reduction Potential ORP in mV range Relative mV feature is also provided pH measurements are compensated for temperature effect manually or automatically with the HI 7669 2W temperature probe One two or three point calibration is possible using five memorized ...

Page 4: ... OFFswitch 11 Powersupplysocket 12 BNCelectrodeconnector 13 Electrodereferencesocket 14 Temperature probe socket 15 RS232serialcommunicationconnector FUNCTIONAL DESCRIPTION Front Panel Primary LCD Secondary LCD 1 9 3 2 7 4 6 5 10 8 POWER 12VDC INPUT REF TEMP 11 12 13 14 Rear Panel C CAL CFM READY 1 2 3 BUF WRONG LOG mV pH TIME DATE INTV BUFFER pH RS232 15 Functions pH Calibration 3 points RCL LOG ...

Page 5: ... 1 0 0 1 8 1 9 1 0 7 6 8 6 1 0 4 e r u t a r e p m e T n o i t a s n e p m o c m o r f c i t a m o t u A r o l a u n a M C º 0 0 2 1 o t 9 9 e d o r t c e l E H p B 1 3 1 1 I H e b o r p e r u t a r e p m e T W 2 9 6 6 7 I H e c n a d e p m i t u p n I 0 1 2 1 m h o y l p p u s r e w o P r e t p a d a C D V 2 1 s n o i s n e m i D 9 2 x 1 7 x 4 9 m m 4 7 x 2 8 1 x 0 4 2 t h g i e W b l 5 2 g K 1 1...

Page 6: ... n o i t a r b i l a c t n i o p 3 r o 2 1 b a l i a v a e l 1 0 0 1 8 1 9 1 0 7 6 8 6 1 0 4 e r u t a r e p m e T n o i t a s n e p m o c m o r f c i t a m o t u A r o l a u n a M C º 0 0 2 1 o t 9 9 e d o r t c e l E H p B 1 3 1 1 I H e b o r p e r u t a r e p m e T W 2 9 6 6 7 I H e c n a d e p m i t u p n I 0 1 2 1 m h o y l p p u s r e w o P r e t p a d a C D V 2 1 s n o i s n e m i D 9 2 x 1...

Page 7: ...ic temperature compensation connect the temperature probe to the appropriate socket INSTRUMENT START UP TurntheinstrumentonbypressingtheON OFFswitch All LCD tags are displayed and a beep is sounded while the instrument performs a self test pH MEASUREMENTS Make sure the instruments have been calibrated before taking pH measurements Submerge the electrode tip and the temperature probe approximately ...

Page 8: ... 25 ºC or the last temperature reading with the ºC tag blinking The temperature can now be adjusted with the ARROW keys from 9 9ºCto120 0ºC mV ORP MEASUREMENTS HI 251 only An optional ORP electrode must be used to perform ORP measurements seeAccessories Oxidation ReductionPotential REDOX measurementsprovidethequantification of the oxidizing or reducing power of the tested sample To correctly perfo...

Page 9: ...eading displayed by the instrument is equal to the difference between the current mV input value and relative mV offset established in the relative mV calibration TEMPERATURE MEASUREMENTS ConnecttheHI7669 2W temperatureprobetotheappropriate socket and turn the instrument on Immerse the temperature probe into the sample and allow the reading on the secondary LCD to stabilize mV C All manuals and us...

Page 10: ...gintheacidicrange usepH7 01or6 86asfirstbufferand pH 4 01 as second buffer If you are measuring in the alkaline range use pH 7 01 or 6 86 as first buffer and pH 10 01 or 9 18 as second buffer PROCEDURE Calibration has a choice of 5 memorized buffers pH 4 01 6 86 7 01 9 18 and 10 01 It is recommended to perform atwoor three point calibration However the instruments also allow one point calibration ...

Page 11: ... reading is stable Whenthereadingisstableandclosetotheselectedbuffer the READY tagwillbedisplayedandthe CFM tagwillblink Press CFM to confirm calibration The calibrated value will be displayed on the primary LCD and the third expected buffer value on the secondary LCD line Note Theinstrumentswillautomaticallyskipthebuffersusedforthefirstand second calibration points After the second calibration po...

Page 12: ...fined temperature range of the buffer Calibration cannot be confirmed in this situation Press RANGEtodisplaythetemperaturereadingon the LCD during calibration TWO POINT CALIBRATION Proceed as described in Three point calibration section Press CAL after the second calibration point was confirmed The instruments will return to measurement mode and will memorize the two point calibration data ONE POI...

Page 13: ...scheckifthetime outtimehasexpired Ifthetimeelapsed the CAL INTV tags will blink as a reminder Note If the instruments were not calibrated the CAL INTV tags will be displayed even if the feature is disabled in SETUP menu LAST CALIBRATION DATA The last calibration data is stored automatically after a successful calibration ToviewthepHcalibrationdata pressGLPwhileinnormalmeasurementmode The instrumen...

Page 14: ...t to see calibration date or time if old calibration was made in the same day with current calibration If no bUF message appears on the LCD the instruments inform you that calibration was performed in less than three points pH resolution during calibration Calibration Alarm Time Out status if disabled or the number of days until the calibration alarm will be displayed or if expired 7 days ago CAL ...

Page 15: ...tember 14 If there are less than 6 memory locations remaining the record number and Lo message will blink to alert the user If the log space is full FULL LOC message will be displayed and no more data will be saved When LOGis pressed a complete set of information is stored date time pH mV temperature and calibration data CAL LOG LOG LOG The instrument ID Note Press GLP at any moment and the instru...

Page 16: ...eter but for a different record Press RANGE and the instruments will display the next logged parameter as follows The mV value on the primary LCD and the record number on the secondary LCD HI251only The temperature value on the primary LCD and the record number on the secondary LCD Note When the pH mV or temperature is displayed if SET CLR is pressed and hold down the secondary LCD will display th...

Page 17: ...ndary LCD along with LOG tag Note If one of the ARROW keys is pressed while dEL and record number is displayed the next previous record number is selected If LOG is pressed the secondary LCD will display ALL message Pressing LOG again the secondary LCD will display the record number Press SET CLR to delete the selected or all records If del ALL option was selected all records are deleted and the i...

Page 18: ...ameter will start blinking Press RANGE to toggle between displayed parameters Press the ARROW keys to increase or decrease the displayed value Press CFM to save the modified value or CAL to escape Press the ARROW keys to select the next previous parameter CURRENT TIME SET Press CAL when the current time is displayed The hour will start blinking Press the ARROW keys to change the hour Press RANGE T...

Page 19: ... to change the day Press CFM to save the modified value Press CAL to escape without saving pH RESOLUTION SET Press CAL when the pH range is displayed The pH range 0 01 or 0 1 will start blinking Press the ARROW keys to change the pH range Press CFM to save the modified pH range Press CAL to escape without saving BEEP STATUS SET Press CAL when the beep status is displayed Beep status On or OFF will...

Page 20: ... is displayed The command prefix 0 to 47 will start blinking Press the ARROW keys to change the command prefix Press CFM to save the modified command prefix value Press CAL to escape without saving Note SeethePCinterfacesectiononpage25foracompleteexplanation Thecommandprefixmustbe16ifHI92000PCsoftwareisused CALIBRATION ALARM TIME OUT SET Press CAL when the calibration time out is displayed Calibra...

Page 21: ...t blinking Press the ARROW keys to change the instrument ID value Press CFM to save the modified instrument ID value Press CAL to escape without saving Note The instrument ID is downloaded to a PC as part of a logged data set to identify its origin All manuals and user guides at all guides com a l l g u i d e s c o m ...

Page 22: ...nsulation material around the vessels to minimize temperature changes Use a calibrated thermometer with a resolution of 0 1 ºC as a reference thermometer With the instruments off press and hold down the CAL LOG keys then power on the instruments The CAL tag will appear and the secondary LCD will show 0 0 ºC Immerse the temperature probe in the vessel with ice and water as near aspossibletothe refe...

Page 23: ... instructions below A two or three point calibration can be performed at 0 0 mV 600 0 mV and 1800 0 mV AttachtotheBNCconnectoramVsimulatorwithanaccuracyof 0 1mV With the instruments off press and hold downthe CFM RCL keys then power on the instruments The CAL tag will appear and the secondary LCD will show 0 0 mV Set 0 0 mV on the simulator When the reading is stable and close to the selected cali...

Page 24: ... is displayed on the secondary LCD When the absolute reading is stable and in measurement range the instrument asks for confirmation If the reading is out of range WRONG tag will be displayed Press CFM to confirm the absolute value The instrument will display 0 0 mV on the primary LCD and rEL message on the secondary LCD In this moment the relative mV offset is equal to absolute mV reading UsetheA...

Page 25: ...leaseseethefollowinginstructions SENDING COMMANDS FROM PC It is also possible to remotely control the instrument with any terminal program Use HI 920010 cable to connect the instrument to a PC start the terminal program and set the communication options as follows 8 N 1 no flowcontrol COMMAND TYPES To send a command to the instrument the scheme is commandprefix command CR where command prefix is a...

Page 26: ...h the current range pH mV and temperature reading on pH range mV and temperature reading on mV range Rel mV absolute mV and temperature reading on Rel mV range MDR Requests the instrument model name and firmware code PAR Requests the setup parameters setting instrument ID calibra tion alarm time out pH range beep status NSL Requests the number of logged samples GLP Requests the calibration data re...

Page 27: ... 1 0 0 1 0 2 3 1 0 4 8 9 6 3 1 7 6 4 9 2 3 0 1 5 1 4 0 0 4 5 9 6 0 1 7 9 3 9 4 2 0 1 0 1 0 5 0 0 4 2 9 6 7 0 7 3 3 9 8 1 0 1 5 1 9 5 0 0 4 0 9 6 4 0 7 7 2 9 2 1 0 1 0 2 8 6 0 0 4 8 8 6 3 0 7 2 2 9 6 0 0 1 5 2 7 7 1 0 4 6 8 6 1 0 7 8 1 9 1 0 0 1 0 3 6 8 2 0 4 5 8 6 0 0 7 4 1 9 6 9 9 5 3 5 9 3 0 4 4 8 6 9 9 6 0 1 9 2 9 9 0 4 4 0 1 4 0 4 4 8 6 8 9 6 7 0 9 8 9 9 5 4 3 1 1 5 0 4 3 8 6 8 9 6 4 0 9 5 8 9...

Page 28: ...ring transport tiny bubbles of air may form inside the glass bulb affecting proper functioning of the electrode These bubbles can be removed by shaking down the electrode as you would do with a glass thermometer Ifthebulband orjunctionisdry soaktheelectrodein HI70300orHI80300 Storage Solution for at least one hour ELECTRODE CONDITIONING MAINTENANCE All manuals and user guides at all guides com ...

Page 29: ...nction should be kept moist and not allowed to dry out Replace the solution in the protective cap with a few drops of HI 70300 or HI 80300 Storage Solution or in its absence Filling Solution HI 7071 or HI 8071 for single junction and HI 7082 or HI 8082 for double junction electrodes Follow the Preparation Procedure on page 28 before taking measurements Note NEVER STORE THE ELECTRODE IN DISTILLED O...

Page 30: ...lution for 15 minutes Oil grease Rinse with Hanna HI 7077 or HI 8077 Oil and Fat Cleaning Solution IMPORTANT After performing any of the cleaning procedures rinse the electrode thoroughly with distilled water refill the reference chamber with freshelectrolyte notnecessaryforgel filledelectrodes andsoaktheelectrode in HI 70300 or HI 80300 Storage Solution for at least 1 hour before taking measureme...

Page 31: ...d n a H p g n i k n i l b 0 0 6 1 H p e h t n i e g n a r f o t u O e l a c s r e t e m e h t e t a r b i l a c e R a H p e h t e r u s e k a M b d e i f i c e p s e h t n i s i e l p m a s e g n a r e t y l o r t c e l e e h t k c e h C c l a r e n e g e h t d n a l e v e l e d o r t c e l e e h t f o e t a t s s w o h s y a l p s i d e h t f I r o 0 0 0 2 d n a V m g n i k n i l b 0 0 0 2 e h t ...

Page 32: ...ce of glass electrodes partially depends on the temperature The lower the temperature the higher the resistance It takes more time for the reading to stabilize if the resistance is higher In addition the response time will suffer to a greater degree at temperatures below 25 ºC SincetheresistanceofthepHelectrodeisintherangeof50 200Mohm the current across the membrane is in the pico Ampere range Lar...

Page 33: ... Solution 460 mL HI 80300L Storage Solution in FDA approved bottle 460 mL ELECTRODECLEANINGSOLUTIONS HI 70000P Electrode Rinse Sachets 20 mL 25 pcs HI 7061L General Cleaning Solution 460 mL HI 7073L Protein Cleaning Solution 460 mL HI 7074L Inorganic Cleaning Solution 460 mL HI 7077L Oil Fat Cleaning Solution 460 mL HI 8061L General Cleaning Solution in FDA approved bottle 460 mL HI 8073L Protein ...

Page 34: ...body tripleceramic conicshape refillable combinationpHelectrode Use emulsions HI 1083B Glass body micro Viscolene non refillable combinationpHelectrode Use biotechnology micro titration HI 1131B Glass body singlejunction refillable combination pHelectrode Use general purpose 120 mm 4 7 12 mm 0 5 9 5mm DIA 0 37 HI 1040 S VERSION HI 1043 120 mm 4 7 12 mm 0 5 HI 1050 S VERSION HI 1053 120 mm 4 7 12 m...

Page 35: ...2031B Glass body semimicro conic refillable combination pHelectrode Use semisolidproducts HI 1332B Plastic body Ultem doublejunction refillable combinationpHelectrode Use general purpose 120 mm 4 7 5mm 0 2 5mm DIA 0 2 HI 1310 S VERSION HI 1330 120 mm 4 7 12 mm 0 5 HI 1210 S VERSION HI 1230 75 mm 2 95 6 mm 0 25 HI 2020 S VERSION HI 2031 120 mm 4 7 12 mm 0 5 HI 1312 S VERSION HI 1332 210 mm 8 25 8 m...

Page 36: ...Kynar open junction conic Viscolene non refillable combination pH electrode Use meat cheese FC 210B Glass body doublejunction conic Viscolene non refillable combination pH electrode Use milk yogurt FC 220B Glass body triple ceramic single junction refillable combination pH electrode Use foodprocessing FC 911B Plastic body Kynar doublejunction refillablewithbuilt inamplifier combination pH electrod...

Page 37: ...ectrode Use titration HI 3230B Plastic body Ultem gel filled combinationplatinum ORPelectrode Use general purpose HI 4430B Plastic body Ultem gel filled combinationgold ORPelectrode Use general purpose ConsulttheHannaGeneralCatalogformoreelectrodeswithscrew typeor BNCconnectors 110 mm 4 3 12 mm 0 5 HI 1413 150 mm 5 9 12 mm 0 5 HI 3111 S VERSION HI 3131 120 mm 4 7 12 mm 0 5 HI 3230 HI 3210 S VERSIO...

Page 38: ... HI 710014 Voltage adapter from 230 VAC to 12 VDC Australiaplug HI 76405 Electrodeholder HI 8427 pH and ORP electrode simulator with 1 m 3 3 coaxial cable ending in female BNC connectors HI 931001 pH and ORP electrode simulator with LCD and 1 m 3 3 coaxial cable ending in female BNC connectors HI7669 2W Temperature probe with 1 m 3 3 cable HI 92000 Windows compatiblesoftware HI 920010 9 to 9 pin R...

Page 39: ... electrode is sensitive to electrostatic discharges Avoid touching this glass bulb at all times During operation ESD wrist straps should be worn to avoid possible damage to the electrode by electrostatic discharges Any variation introduced by the user to the supplied equipment may degrade the instruments EMC performance To avoid electrical shock do not use these instruments when voltages at the me...

Page 40: ... Fax 03 3258 9567 Korea Tel 02 2278 5147 Fax 02 2264 1729 Malaysia Tel 603 5638 9940 Fax 603 5638 9829 Singapore Tel 6296 7118 Fax 6291 6906 SouthAfrica Tel 011 615 6076 Fax 011 615 8582 Taiwan Tel 886 2 2739 3014 Fax 886 2 2739 2983 Thailand Tel 662 619 0708 11 Fax 662 619 0061 UnitedKingdom Tel 01525 850 855 Fax 01525 853 668 USA Tel 401 765 7500 Fax 401 765 7575 For e mail contacts and a comple...

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