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Hanna Instruments C 216, Instruction Manual, Page: 32 / 52

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32

10 mL

SPECIFICATIONS

Range

0.00 to 5.00 mg/L

Resolution

0.01 mg/L

Precision

±0.03 mg/L @ 1.00 mg/L

Typical EMC

±0.01 mg/L

Deviation

Light Source

Tungsten lamp with narrow band interference filter

@ 575 nm

Method

Adaptation of the USEPA approved method. The

reaction between free copper and the bicinchoninate

reagent causes a purple tint in the sample.

REQUIRED REAGENTS

Code

Description

Quantity/test

HI  93702-0

Bicinchoninate

1 packet

REAGENT SETS

HI 93702-01

Reagents for 100 tests

HI 93702-03

Reagents for 300 tests

For other accessories see page 49.

MEASUREMENT PROCEDURE

• Select the program number corresponding to Free Copper on the

secondary LCD by pressing PROGRAM 

▼

 and 

▲

.

• Fill the cuvet with 10 mL of unreacted

sample, up to the mark, and replace
the cap.

• Place the cuvet into the holder and

ensure that the notch on the cap is
positioned securely into the groove.

• Press ZERO and “SIP” will blink on the display.

FREE COPPER

Free Copper

Summary of Contents for C 216

Page 1: ...1 Instruction Manual C 216 C 226 Multiparameter Bench Photometers for Pool Spa Applications w w w h a n n a i n s t c o m These Instruments are in Compliance with the CE Directives ...

Page 2: ...ING 5 SPECIFICATIONS 10 PRECISION AND ACCURACY 10 PRINCIPLE OF OPERATION 11 FUNCTIONAL DESCRIPTION 13 GUIDE TO DISPLAY CODES 14 TIPS FOR AN ACCURATE MEASUREMENT 17 PARAMETERS REFERENCE TABLES 19 OPERATIONAL GUIDE 20 ALKALINITY 22 BROMINE 24 CALCIUM HARDNESS 26 FREE CHLORINE 28 TOTAL CHLORINE 30 FREE COPPER 32 TOTAL COPPER 34 CYANURIC ACID 36 IRON 38 OZONE 40 pH 44 INTERFACE WITH PC 46 STANDARD MET...

Page 3: ...nt please notify your Dealer Each Meter is supplied complete with Four Sample Cuvets and Caps Two 9V Batteries One pair of scissors Instruction Manual Rigid carrying case Note Save all packing material until you are sure that the instrument works correctly Any defective item must be returned in its original packing with the supplied accessories ...

Page 4: ...ers use an exclusive positive locking system to ensure that the cuvet is in the same position every time it is placed into the measurement cell The reagents are in liquid or powder form and are supplied in bottles or in packets The amount of reagent is precisely dosed to ensure the maximum repeatability Display codes aid the user in routine operations The meters have an auto shut off feature that ...

Page 5: ...one dosing systems of which chlorine is the most common Chlorine Chlorineisastrongoxidizingagentthatdestroysmostlyorganicpollutants bacteria and can combine with nitrogen containing compounds forming chloramines Only a part of the original quantity dosed chlorine remains active and continues its disinfecting action From the freechlorine you can distinguish combinedchlorine asthat part which combin...

Page 6: ...anitizer while the molecular HOCl is a fast acting it is important to measure regularly the pH AsageneralruleapHofabout7 2isrecommendedtomaintainfast acting disinfection conditions Bromine In many countries bromine sanitizing has been introduced as an alternativeforchlorine althoughitisalessstrongsanitizer Theadvantage of bromine is its stability at higher temperatures advantageous for hot well po...

Page 7: ... other hand scaling does cause failure because of incrustation problems In the treatment and monitoring of pool water the pool manager must ensure that related parameters as alkalinity hardness and pH are duly taken into consideration Calcium Hardness The presents of calcium in the system is desired to ensure filming on those places where the temperature is relatively high like in boilers and pipe...

Page 8: ...Alkalinity ppm as CaCO3 TocalculatetheexactLangelierIndexofyourwaterpleaseusetheWATER INDEX referencetablesattheendofthischaptertofindtheTemperature Hardness and Alkalinity factors Recommendations For most pools water is balanced if The pH value is maintained within the recommended ranges of pH 7 2 7 6 Ideally the Alkalinity should be maintained within a range of 80 125 ppm The Calcium Hardness sh...

Page 9: ... 4 8 12 16 20 24 28 32 36 40 50 F 32 39 46 54 60 68 75 82 90 97 104 122 TF 0 0 1 0 2 0 3 0 4 0 5 0 6 0 7 0 7 0 8 0 9 1 0 mg L as CaCO3 5 25 50 75 100 150 200 250 300 400 500 1000 HF 0 7 1 4 1 7 1 9 2 0 2 2 2 3 2 4 2 5 2 6 2 7 3 0 mg L as CaCO3 5 25 50 75 100 150 200 250 300 400 500 1000 AF 0 7 1 4 1 7 1 9 2 0 2 2 2 3 2 4 2 5 2 6 2 7 3 0 Temperature Calcium Hardness Alkalinity EXAMPLE Water Balance...

Page 10: ...to the related measurement section PRECISION AND ACCURACY Precision is how closely repeated measurements agree with each other Precision is usually expressed as standard deviation SD Accuracy is defined as the nearness of a test result to the true value Although good precision suggests good accuracy precise results can be inaccurate The figure explains these definitions Foreachparameter theprecisi...

Page 11: ...ubstance d optical path through the substance Therefore the concentration c can be calculated from the absorbance of the substance as the other factors are known Photometric chemical analysis is based on the possibility to develop an absorbing compound from a specific chemical reaction between sample and reagents Given that the absorption of a compound strictly depends on the wavelength of the inc...

Page 12: ... LCD The measurement process is carried out in two phases first the meter is zeroed and then the actual measurement is performed The cuvet has a very important role because it is an optical element and thus requires particular attention It is important that both the measurement and the calibration zeroing cuvets are optically identical to provide the same measurement conditions Whenever possible u...

Page 13: ...urement immediately 5 TIMER to perform measurement after a preprogrammed countdown 6 ZERO to zero the meter prior to measurement 7 Program and to select the desired parameter 8 ON OFF to turn the meter on and off REAR PANEL 1 Power Supply 12 VDC 2 5 Watt 2 RS 232 Socket 3 Batteries Compartment FUNCTIONAL DESCRIPTION ...

Page 14: ... meter is in a zeroed stateandmeasurementcanbeperformed The blinking LOBAT indicates that the battery voltage is getting low and the batteries need to be replaced The instrument is performing an internal check up This indicates that the meter is in a ready state and zeroing can be performed This indicates that the batteries are dead and must be replaced Light over range The cuvet is not inserted c...

Page 15: ...easecheckthepreparation of the zero cuvet This indicates that the zeroing procedure failedduetoalowsignal to noiseratio In this case press ZERO again There is too much light to perform a measurement Pleasecheckthepreparation of the zero cuvet The lamp is not working properly Contact yourdealerorthenearestHannaCustomer Service Center The lamp is not working properly Contact yourdealerorthenearestHa...

Page 16: ...nd make sure that you use the same cuvet for reference zero and measurement 1 A flashing value of the maximum concentration indicates an over range condition Theconcentrationofthesample is beyond the programmed range dilute the sample and rerun the test 2 A flashing value lower than the maximum concentration indicates a low signal to noiseratiocondition Inthiscase accuracy of the result is not gua...

Page 17: ... at the same level of the 10 mL mark Proper use of the dropper a to get good reproducible results tap the dropper on the table for several times and wipe the outside of the dropper tip with a cloth b always keep the dropper bottle in a vertical position while dosing the reagent Proper use of the powder reagent packet a use scissors to open the powder packet b push the edges of the packet to form a...

Page 18: ...haking the cuvet can generate bubbles in the sample causing higher readings To obtain accurate measurements remove such bubbles by swirling or by gently tapping the vial Do not let the reacted sample stand too long after reagent is added or accuracy will be lost It is possible to take multiple readings in a row but it is recommended to take a new zero reading for each sample and to use the same cu...

Page 19: ...Alkalinity 22 2 Ca Hardness 26 3 Free Chlorine 28 4 Total Chlorine 30 5 Cyanuric Acid 36 6 pH 44 Code Parameter Page 1 Alkalinity 22 2 Bromine 24 3 Ca Hardness 26 4 Free Chlorine 28 5 Total Chlorine 30 6 Free Copper 32 7 Total Copper 34 8 Cyanuric Acid 36 9 Iron 38 10 Ozone 40 11 pH 44 ...

Page 20: ... by pressing ON OFF The meter will first perform an LCD auto diagnostic test by displaying a full set of figures Then it will show a scrolling c Hanna Inst whereas the meter will indicate the exact meter code C 216 or C 226 When the LCD displays the meter is ready On the secondary LCD P1 will appear to inform that the first parameter measurement procedure Alkalinity both for C 216 and C 226 can be...

Page 21: ...Select a new parameter measurement procedure by pressing the PROGRAM and PROGRAM keys Note in the following measurement sections a generic P will be placed on the secondary LCD instead of the exact related message e g in C 226 P2 for Bromine Before performing a test read carefully all the instructions related to the selected parameter ...

Page 22: ...iption Quantity test HI 93755 0 Alkalinity Indicator Reagent 1 packet REAGENT SETS HI 93755 01 Reagents for 100 tests HI 93755 03 Reagents for 300 tests For other accessories see page 49 MEASUREMENT PROCEDURE Select the program number corresponding to Alkalinity on the secondary LCD by pressing PROGRAM and Fill the cuvet with 10 mL of unreacted sample up to the mark and replace the cap Place the c...

Page 23: ...tention not to spill reagent otherwise full color development may be inhibithed Replace the cuvet into the holder and ensure that the notch on the cap is positioned securely into the groove Press TIMER and the display will show the countdown prior to measurement Alternatively wait for 2 minutes and press READ DIRECT In both cases SIP will blink during measurement The instrument directly displays t...

Page 24: ...ple REQUIRED REAGENTS Code Description Quantity test HI 93716 0 DPD Powder Reagent 1 packet REAGENT SETS HI 93716 01 Reagents for 100 tests HI 93716 03 Reagents for 300 tests For other accessories see page 49 MEASUREMENT PROCEDURE Select the program number corresponding to Bromine on the secondary LCD by pressing PROGRAM and Fill the cuvet with 10 mL of unreacted sample up to the mark and replace ...

Page 25: ...rior to measurement Alternatively wait for 2 minutes and 30 seconds and press READ DIRECT In both cases SIP will blink during measurement The instrument directly displays concentration in mg L of bromine on the Liquid Crystal Display INTERFERENCES Interference may be caused by Chlorine Chlorine Dioxide Ozone In case of water with hardness greater than 500 mg L CaCO3 shake the sample for approximat...

Page 26: ...agent 1 packet REAGENT SETS HI 93756 01 Reagents for 100 tests HI 93756 03 Reagents for 300 tests For other accessories see page 49 MEASUREMENT PROCEDURE Select the program number corresponding to calcium Hardness on the secondary LCD by pressing PROGRAM and Fill the cuvet with 10 mL of unreacted sample up to the mark and replace the cap Place the cuvet into the holder and ensure that the notch on...

Page 27: ...he cap is positioned securely into the groove Press TIMER and the display will show the countdown prior to measurement Alternatively wait for 2 minutes and press READ DIRECT In both cases SIP will blink during measurement The instrument directly displays concentration in mg L of calcium as CaCO3 on the Liquid Crystal Display To convert the result to mg L of calcium Ca2 multiply by a factor of 0 4 ...

Page 28: ...reagent causes a pink tint in the sample REQUIRED REAGENTS Code Description Quantity test HI 93701 0 DPD Powder Reagent 1 packet REAGENT SETS HI 93701 01 Reagents for 100 tests HI 93701 03 Reagents for 300 tests For other accessories see page 49 MEASUREMENT PROCEDURE Select the program number corresponding to Free Chlorine on the secondary LCD by pressing PROGRAM and Fill the cuvet with 10 mL of u...

Page 29: ... DIRECT SIP will blink during measurement The instrument directly displays concentration in mg L of free chlorine on the Liquid Crystal Display INTERFERENCES Interference may be caused by Bromine Chlorine Dioxide Iodine Ozone all these interferences give positive errors Alkalinity above 250 mg L CaCO3 will not reliably develop the full amount of color or it may rapidly fade To resolve this neutral...

Page 30: ...nt causes a pink tint in the sample REQUIRED REAGENTS Code Description Quantity test HI 93711 0 DPD Powder Reagent 1 packet REAGENT SETS HI 93711 01 Reagents for 100 tests HI 93711 03 Reagents for 300 tests For other accessories see page 49 MEASUREMENT PROCEDURE Select the program number corresponding to Total Chlorine on the secondary LCD by pressing PROGRAM and Fill the cuvet with 10 mL of unrea...

Page 31: ... for 2 minutes and 30 seconds and press READ DIRECT In both cases SIP will blink during measurement The instrument directly displays concentration in mg L of total chlorine on the Liquid Crystal Display INTERFERENCES Interference may be caused by Bromine Chlorine Dioxide Iodine Ozone all these interferences give positive errors Alkalinity above 250 mg L CaCO3 will not reliably develop the full amo...

Page 32: ...TS Code Description Quantity test HI 93702 0 Bicinchoninate 1 packet REAGENT SETS HI 93702 01 Reagents for 100 tests HI 93702 03 Reagents for 300 tests For other accessories see page 49 MEASUREMENT PROCEDURE Select the program number corresponding to Free Copper on the secondary LCD by pressing PROGRAM and Fill the cuvet with 10 mL of unreacted sample up to the mark and replace the cap Place the c...

Page 33: ...y for 15 seconds Replace the cuvet into the holder and ensure that the notch on the cap is positioned securely into the groove Press TIMER and the display will show the countdown prior to measurement Alternatively wait for 45 seconds and press READ DIRECT In both cases SIP will blink during measurement The instrument directly displays concentration in mg L of free copper on the Liquid Crystal Disp...

Page 34: ...ntity test HI 93702 0 Bicinchoninate 1 packet HI 93702T 0 Decomplexing Agent 1 packet REAGENT SETS HI 93702T 01 Reagents for 100 tests HI 93702T 03 Reagents for 300 tests For other accessories see page 49 MEASUREMENT PROCEDURE Select the program number corresponding to Total Copper on the secondary LCD by pressing PROGRAM and Fill the cuvet with 10 mL of unreacted sample up to the mark and replace...

Page 35: ...0 Copper Total Reagent Replace the cap and shake vigorously for 15 seconds Replace the cuvet into the holder and ensure that the notch on the cap is positioned securely into the groove Press TIMER and the display will show the countdown prior to measurement Alternatively wait for 45 seconds and press READ DIRECT In both cases SIP will blink during measurement The instrument directly displays conce...

Page 36: ... Description Quantity test HI 93722 0 Cyanuric Acid Reagent 1 packet REAGENT SETS HI 93722 01 Reagents for 100 tests HI 93722 03 Reagents for 300 tests For other accessories see page 49 MEASUREMENT PROCEDURE Select the program number corresponding to Cyanuric Acid on the secondary LCD by pressing PROGRAM and Fill the cuvet with 10 mL of unreacted sample up to the mark and replace the cap Place the...

Page 37: ...he cuvet into the holder and ensure that the notch on the cap is positioned securely into the groove Press TIMER and the display will show the countdown prior to measurement Alternatively wait for 45 seconds and press READ DIRECT In both cases SIP will blink during measurement The instrument directly displays concentration in mg L of cyanuric acid on the Liquid Crystal Display INTERFERENCES Turbid...

Page 38: ...n orange tint in the sample REQUIRED REAGENTS Code Description Quantity test HI 93721 0 Iron High Range Reagent 1 packet REAGENT SETS HI 93721 01 Reagents for 100 tests HI 93721 03 Reagents for 300 tests For other accessories see page 49 MEASUREMENT PROCEDURE Select the program number corresponding to Iron on the secondary LCD by pressing PROGRAM and Fill the cuvet with 10 mL of unreacted sample u...

Page 39: ...into the groove Press TIMER and the display will show the countdown prior to measurement Alternatively wait for 3 minute and press READ DIRECT In both cases SIP will blink during measurement Note for the majority of samples a reading may be taken already after 1 minute because color development in the sample is in general very fast full color development within 1 minute The instrument directly dis...

Page 40: ... Reagents for 100 tests HI 93757 03 Reagents for 300 tests HI 93703 52 Glycine Powder Optional Reagent for 100 tests For other accessories see page 49 IMPORTANT NOTE chlorine is a strong positive interference for the ozone determination If the sample is suspected to contain chlorine residues free or total chlorine please follow the alternative measurement procedure described below Perform the Stan...

Page 41: ...play will show 0 0 Now the meter is zeroed and ready for measurement Remove the cuvet Add the content of one packet of HI 93757 0 Ozone Reagent Replace the cap and shake gently for 20 seconds Replace the cuvet into the holder and ensure that the notch on the cap is positioned securely into the groove Press TIMER and the display will show the countdown prior to measurement Alternatively wait for 2 ...

Page 42: ...groove Press ZERO and SIP will blink on the display Wait for a few seconds and the display will show 0 0 Now the meter is zeroed and ready for measurement Remove the cuvet Add the content of one packet of the optional reagent HI 93703 52 0 Glycine Powder Replace the cap and shake gently until dissolution is complete Add the content of one packet of HI 93757 0 Ozone Reagent Replace the cap and shak...

Page 43: ...tract this value from the reading from the Standard Measurement Procedure this will be the concentration in mg L of ozone in the sample INTERFERENCES Interference may be caused by Bromine Chlorine Dioxide Iodine Alkalinity above 250 mg L CaCO3 will not reliably develop the full amount of color or it may rapidly fade To resolve this neutralize the sample with diluted HCl In case of water with hardn...

Page 44: ... Description Quantity test HI 93710 0 Phenol Red Indicator 1 packet REAGENT SETS HI 93710 01 Reagents for 100 tests HI 93710 03 Reagents for 300 tests For other accessories see page 49 MEASUREMENT PROCEDURE Select the program number corresponding to pH on the secondary LCD by pressing PROGRAM and Fill the cuvet with 10 mL of unreacted sample up to the mark and replace the cap Place the cuvet into ...

Page 45: ...t Add 5 drops of HI 93710 0 pH Reagent Replace the cap and swirl the cuvet Replace the cuvet into the holder and ensure that the notch on the cap is positioned securely into the groove Press READ DIRECT and SIP will blink during measurement The instrument directly displays the pH measured value on the Liquid Crystal Display ...

Page 46: ... to 2400 If you wish to change this value please contact your nearest Hanna Center SENDING COMMANDS FROM PC With terminal programs such as for example Telix Windows Termi nal it is possible to remotely control your Hanna Instruments bench meter Use HI 920010 cable to connect the meter to the PC start the terminal program and set the communication options as follows 8 N 1 no flow control Command Ty...

Page 47: ...Lock Keyboard KBU Unlock Keyboard PR Send Current Program Number BRQ Send current baud rate 1 150 2 300 3 600 4 1200 5 2400 CNQ Send Concentration three bytes conc decimal point unit m ppm b ppb t ppt u pcu h pH ERR send error status information 0 No error 1 CAP 2 HI 3 ZERO 4 LO 5 IDLE 6 ZERO DONE 7 TIMED READ ...

Page 48: ...tes of non use A blinking LOBAT on the display while a measurement is in progress indicates low voltage and the batteries should be replaced If the batteries are not replaced immediately in order to prevent erroneous readings due to low voltage BA is displayed soon afterwards At this point the batteries must be changed Battery replacement must only take place in a non hazardous area using two 9V a...

Page 49: ...755 03 300 alkalinity tests HI 93756 01 100 calcium hardness tests HI 93756 03 300 calcium hardness tests HI 93757 01 100 ozone tests HI 93757 03 300 ozone tests OTHER ACCESSORIES C115 00300 5 mL graduated syringe HI 710005 110VAC to 12VDC voltage adapter HI 710006 220VAC to 12VDC voltage adapter HI 721310 9V battery 10 pcs HI 731318 Tissue for wiping cuvets 4 pcs HI 731321 Glass cuvets 4 pcs HI 7...

Page 50: ... interferences to other electronic equip ments this requiring the operator to take all necessary steps to correct interferences Any variation introduced by the user to the supplied equipment may degrade the instruments EMC performance To avoid damages or burns do not put the instrument in microwave ovens For yours and the instrument safety do not use or store the instrument in hazardous environmen...

Page 51: ...Service Department and then send it with shipment costs prepaid When shipping any instrument make sure it is properly packaged for complete protection To validate your warranty fill out and return the enclosed warranty card within 14 days from the date of purchase Hanna Instruments reserves the right to modify the design construction and appearance of its products without advance notice HANNA LITE...

Page 52: ...52 MANC216R2 01 03 w w w h a n n a i n s t c o m ...

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