A: Calibrating the Wavelength of the QE65000
7. Record the Intercept, as well as the First, Second, and Third Coefficients. Additionally, look at
the value for R squared. It should be very close to 1. If not, you have most likely assigned one of
your wavelengths incorrectly.
Keep these values at hand.
Saving the New Calibration Coefficients: USB
Mode
Ocean Optics programs wavelength calibration coefficients unique to each QE65000 onto an EEPROM
memory chip in the QE65000.
You can overwrite old calibration coefficients on the EEPROM
if you are using the QE65000 via the
USB port.
►
Procedure
To save wavelength calibration coefficients using the USB mode, perform the following steps:
1. Ensure that the QE65000 is connected to the PC and that you have closed all other applications.
2. Point your browser to
http://www.oceanoptics.com/technical/softwaredownloads.asp
scroll down to
Microcode
. Select
USB EEPROM Programmer
.
3. Save the setup file to your computer.
4. Run
the
Setup.exe
file to install the software. The
Welcome
screen appears.
5. Click
the
Next
button. The
Destination Location
screen appears.
6. Accept the default installation location, or click the
Browse
button to specify a directory. Then,
click the
Next
button. The
Program Manager Group
screen appears.
7. Click
the
Next
button. The
Start Installation
screen appears.
8. Click
the
Next
button to begin the installation. Once the installation finishes, the
Installation
Complete
screen appears.
9. Click
the
Finish
button and reboot the computer when prompted.
10. Navigate to the
USB EEPROM Programmer
from the Start menu and run the software.
11. Click on the desired QE65000 device displayed in the left pane of the
USB Programmer
screen.
12. Double-click on each of the calibration coefficients displayed in the right pane of the USB
Programmer screen and enter the new values acquired in Steps 5 and 6 of the
Wavelength of the Spectrometer
section in this appendix.
13. Repeat Step 12 for all of the new values.
14. Click on the
Save All Values
button to save the information, and then
Exit
the USB Programmer
software.
The new wavelength calibration coefficients are now loaded onto the EEPROM memory chip on the
QE65000.
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