Hach PHC729 User Manual Download Page 11

Section 6 Sample measurement

The procedure that follows is applicable to meters that can connect to Intellical pH probes. Refer to
the applicable meter documentation for meter operation and probe-specific settings.

6.1 Sample requirements

N O T I C E

 

Make sure that the sample type is compatible with the probe, or probe damage can occur.

• Samples must be aqueous. The probe can also make measurements in samples that are not fully

aqueous and in some solvents that dissolve in water. Read the results with caution because the
pH scale changes when the solvent system changes.

• Samples can contain components that react with silver (e.g., TRIS, proteins and sulfides). The

Red Rod tubes protect the probe from interference with such components.

• Proteins can collect on the sensing element. Make sure that the probe stays clean if proteins are in

the sample.

• Do not measure samples that are hotter or colder than the specified operating temperature of the

probe. Refer to 

Specifications

 on page 3.

6.2 Sample measurement notes

Read the notes that follow before sample measurements.

• Rinse the probe with deionized water and dry with a lint-free cloth between measurements to

prevent contamination.

• If complete traceability is necessary, enter a sample ID and operator ID before measurement.

Refer to the meter manual for instructions.

• The meter automatically saves the measurement data when the user manually reads each data

point and when the meter is set to read at regular intervals. The user must manually save each
data point when the meter is set to read continuously.

• Air bubbles below the sensor can cause a slow response or error in the measurement. Make sure

to remove air bubbles before and during measurements.

6.3 Sample measurement procedure

1. 

 Open the filling

hole.

2. 

 Rinse the probe

with deionized water.
Dry the probe with a
lint-free cloth.

3. 

 Put the probe tip

on the sample
surface.

4. 

 Read the pH

value of the sample.
The display shows
the temperature-
compensated pH
value when the
reading is stable.

English

   

9

Summary of Contents for PHC729

Page 1: ...DOC022 53 80433 PHC729 05 2021 Edition 2 User Manual...

Page 2: ......

Page 3: ...rocedure 8 Section 6 Sample measurement 9 6 1 Sample requirements 9 6 2 Sample measurement notes 9 6 3 Sample measurement procedure 9 Section 7 Verify the calibration 10 7 1 Verification procedure 10...

Page 4: ...Table of Contents 2...

Page 5: ...r the best accuracy do not use the probe to measure the pH of very alkaline samples Figure 1 Probe overview 1 Probe soaker bottle with storage solution 5 Probe soaker bottle holder 2 Filling hole plug...

Page 6: ...but not improper use or wear Certifications CE FCC ISED Section 3 Safety information 3 1 Intended use The Intellical PHC729 probe is intended for use by individuals in the laboratory who measure the p...

Page 7: ...probe with a blocked filling hole will not operate correctly New probes are filled with electrolyte filling solution and have a soaker bottle that contains storage solution to keep the glass bulb and...

Page 8: ...e meter starts for the first time or after battery replacement 8 Connect the probe to the meter 9 Remove the filling hole plug before use Keep the level of the electrolyte filling solution above the l...

Page 9: ...2 or more pH units apart For a one point calibration use a pH buffer near the expected sample pH Use the default calibration options or change the options in the probe settings menu Use the single di...

Page 10: ...ffer solution Make sure that the sensor and reference junction are fully in the solution Do not put the probe on the bottom or sides of the beaker 6 Shake the probe from side to side to refresh the re...

Page 11: ...e specified operating temperature of the probe Refer to Specifications on page 3 6 2 Sample measurement notes Read the notes that follow before sample measurements Rinse the probe with deionized water...

Page 12: ...ay shows the pH buffer solution to use for verification Note Menu name for HQd meters Run check standard 2 Prepare or pour the pH buffer solution into a beaker 3 Open the filling hole 4 Rinse the prob...

Page 13: ...ater Dry the probe body with a lint free cloth 4 Soak the probe in pH 4 buffer for 20 minutes 5 Rinse the probe with deionized water Dry the probe body with a lint free cloth Table 1 Cleaning solution...

Page 14: ...crystals to the probe The filling solution in the probe contains crystals of potassium chloride KCl that keeps the filling solution saturated with KCl When the sample temperature is more than 60 C 140...

Page 15: ...ttle 2 Measuring scoop 5 Saturated KCl solution 3 KCl crystals Table 2 KCl quantity at high temperatures Temperature KCl quantity 60 C 140 F 0 5 g 0 5 cm 0 2 in 70 C 158 F 0 625 g 80 C 176 F 0 750 g 9...

Page 16: ...d KCl crystals from the probe as possible Discard the solution and KCl crystals 3 Remove the remaining KCl crystals as follows a Fill the probe half full with deionized water Close the filling hole b...

Page 17: ...N O T I C E Probes can become permanently damaged if kept in a storage solution that is not specified by the manufacturer Use only the specified storage solution saturated KCl Do not store the probe i...

Page 18: ...perature difference between samples Increase the sample temperature or adjust the temperature of different samples to be the same within 2 C 3 6 F Procedure problem causes slow stabilization and preve...

Page 19: ...lution kit Singlet one use packets 20 mL each 2 x 10 pkg 2769920 pH 7 00 and 10 01 buffer solution kit Singlet one use packets 20 mL each 2 x 10 pkg 2769820 pH color coded buffer solution kit NIST 500...

Page 20: ...5013 Beaker 30 mL plastic green 80 pkg SM5014 Beaker dispenser and holder 30 mL 1 923 656 Beaker holder 30 mL 1 923 556 Beaker 100 mL polypropylene 1 108042 Filling hole plug clip on 1 M035J35 LIS low...

Page 21: ......

Page 22: ...669 2932 orders hach com www hach com HACH LANGE GMBH Willst tterstra e 11 D 40549 D sseldorf Germany Tel 49 0 2 11 52 88 320 Fax 49 0 2 11 52 88 210 info de hach com www de hach com HACH LANGE S rl 6...

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