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MyPIPETMAN®
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USER’S GUIDE
Leak Test
This test may be performed at any time to check that the pipette does not leak, especially after
performing a maintenance or decontamination procedure. If a pipette fails this test, replace the
O-ring and seal. After making sure that the pipette is correctly reassembled, repeat this test.
For the P2 to P200 single channel models:
1.
Fit a PIPETMAN DIAMOND Tip.
2. Set the pipette to the maximum volume given in the specifications and pre-wet.
3. Aspirate the set volume from a beaker of distilled water.
4. Maintain the pipette in the vertical position and wait for 20 seconds:
○
If a water droplet appears at the end of the tip, there is a leak.
○
If you see no droplets, re-immerse the tip below the surface of water. The water level inside the
tip should remain constant; if the level goes down, there is a leak.
For the P1000, P5000, and P10mL single channel models:
1.
Fit a PIPETMAN DIAMOND tip.
2. Set the pipette to the maximum volume given in the specifications.
3. Aspirate the set volume from a beaker of distilled water.
4. Maintain the pipette in the vertical position and wait for 20 seconds:
○
If a water droplet appears at the end of the tip, there is a leak.
For the multichannel models:
1.
Fit the PIPETMAN DIAMOND tip.
2. Set the pipette to the maximum volume given in the specifications, and pre-wet the tips.
3. Aspirate the set volume from a reagent reservoir of distilled
water.
4. Maintain the pipette in the vertical position and wait for 20
seconds:
○
If a water droplet appears at the end of the tip, there is a
leak.
○
If you see no droplets, for volumes below 200 µL,
re-immerse the tip below the surface of water.
○
The water level inside the tip should remain constant;
there is a leak if the level goes down.
no leak
Figure 10
MyPIPETMAN® multichannel leak test
Chapter 6
CLEANING AND DECONTAMINATION
MyPIPETMAN pipettes are fully autoclavable without disassembly for maximum convenience as well as
protection from contamination.
NOTICE
You may refer to the decontamination procedure available on
Liquid must never enter any pipette’s upper part (handle).
Cleaning for Single Channel Models
The pipette must be cleaned, as described below, before it is decontaminated. Soap solution is
recommended for cleaning MyPIPETMAN.
External
1.
Remove the tip ejector (refer to
Changing the Tip Ejector for Single Channel Models
).
2. Wipe the entire pipette and the tip ejector with a soft-cloth or lint-free tissue soaked with soap
solution to remove all dirty spots or marks. If the pipette is very dirty, a brush with soft, plastic
bristles may be used.
3. Wipe the entire pipette and the tip ejector with a soft cloth or lint-free tissue soaked with distilled
water.
4. Refit the tip ejector and allow the pipette to dry.
