EXTRACTMAN®
|
User’s Guide
Operation
|
EXTRACTMAN®
O
per
ation
17
Input, Wash, and Output Volumes
●
The first well column (460–500 µL) was designed for input wells .
●
The second (230–275 µL) and third (100–110 µL) well columns were
designed for input or wash wells .
●
The fourth and fifth (100–110 µL) well columns were designed for
wash wells .
●
The sixth (10–15 µL) well column was designed for output wells .
NOTE
Well volumes may vary because the formation
of stable, prominent menisci may require the
addition or subtraction of reagent, depending on
the surface tension of the reagent used .
3 . Fill each subsequent well with wash or reagent solution .
NOTE
Wells should have prominent menisci to ensure proper
contact with the bead capture strip .
Figure 10:
Input, Wash, and Output Well Locations
Input
(Blue)
Wash
(Red)
Output
(Green)
Input or Wash
(Purple)
460–500 µL
230–275 µL 100–110 µL
10–15 µL
Figure 11:
Prominent Menisci on the 4-Row Microplate