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EXTRACTMAN®

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 User’s Guide

Operation 

|

 EXTRACTMAN®

O

per

ation

17

Input, Wash, and Output Volumes

 

The first well column (460–500 µL) was designed for input wells .

 

The second (230–275 µL) and third (100–110 µL) well columns were 

designed for input or wash wells .

 

The fourth and fifth (100–110 µL) well columns were designed for 

wash wells .

 

The sixth (10–15 µL) well column was designed for output wells .

NOTE

Well volumes may vary because the formation 

of stable, prominent menisci may require the 

addition or subtraction of reagent, depending on 

the surface tension of the reagent used .

3 .  Fill each subsequent well with wash or reagent solution .

NOTE

Wells should have prominent menisci to ensure proper 

contact with the bead capture strip .

Figure 10: 

Input, Wash, and Output Well Locations

Input

(Blue)

Wash

(Red)

Output 

(Green)

Input or Wash

(Purple)

460–500 µL

230–275 µL 100–110 µL

10–15 µL

Figure 11: 

Prominent Menisci on the 4-Row Microplate

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