GenScript eZwest Lite Manual Download Page 2 | Manualshive

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GenScript eZwest Lite Manual Download Page 2

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Summary of Contents for eZwest Lite

Page 1: ...Automated Western Device...

Page 2: ......

Page 3: ...ons 8 2 1 Instrument display and feature location 8 2 2 Basic operation 10 Save cassette ID when using the cassette for the first time 10 View and adjust method 11 Set cassette specific method 14 2 3...

Page 4: ...2...

Page 5: ...set up the device before your first experiment Note Before relocating the instrument please drain the system completely Step Description 1 Check and make sure the power plug matches the outlet 2 Plac...

Page 6: ...tion done by any other party than GenScript or an authorized agent Use of fittings or other spare parts supplied by any other party than GenScript Damages caused by disasters For consultation and main...

Page 7: ...iability typically associated with manual operations contributing to experimental reproducibility The eZwest Lite device is compatible with the traditional reagents and protocols used for conventional...

Page 8: ...e size WxH 90x83 mm 3 5x3 2 in Throughput 1 channel for up to 2 membranes Application Western blotting eZwest Lite ABS PP stainless steel silicone Forceps Stainless Steel Roller PC 1 3 Ordering inform...

Page 9: ...west device extensive cleaning After every 20 use or every 2 weeks perform the extensive cleaning following the procedures below 1 Empty the Wash bottle and Waste bottle 2 Add 100 mL deionized water i...

Page 10: ...8 2 Instructions 2 1 Instrument display and feature location eZwest Lite automated western device Fig 1 Front view Fig 2 Back view...

Page 11: ...9 Fig 3 Top view Fig 4 Membrane cassette Fig 5 Display main menu...

Page 12: ...shown in Fig 7 2 Switch on the device insert the membrane cassette into the device Make sure the pins are pushed all the way in and the side with the eZwest logo is upright Note The pins are designed...

Page 13: ...ress OK to save cassette ID as show in Fig 8 Note Each eZwest Lite device could save up to 5 cassette IDs Fig 8 View and adjust method 1 Enter method setting by pressing Method on the upper right corn...

Page 14: ...to show and modify the associated details Note Each Method may be programmed with 7 steps Fig 11 4 Each step within a Method is customizable a Reagent option Fig 12 b Number of Cycles required to repe...

Page 15: ...13 c Run time for each cycle in minutes Fig 14 d Pump Speed for recirculating the liquid flow through the membrane cassette Fig 15 5 Once you have finished setting up your Method press Save...

Page 16: ...Change name of membrane cassette a Under parameter interface select the name of membrane cassette Fig 16 b Press Edit ID and change name with the pop up keyboard Fig 17 2 3 Using the Pre programmed b...

Page 17: ...e the latch by rotating it up as shown in Fig 19 i lift the latch and pull it towards the top of the cassette as in Fig 19 ii the opened cassette is shown in Fig 19 iii Fig 19 i ii iii 4 Placing membr...

Page 18: ...of the first membrane along the membrane edge Lay the second membrane on top with the protein side facing down and high MW side align towards the bottom edge of the cassette Make sure both spacers are...

Page 19: ...result in experiment failure 8 Select the method on the main menu and click START to run 9 Once method finish click FINISH and replace the membrane cassette with cleaning cassette Membranes are ready...

Page 20: ...min Normal Wash 4 3 min Normal Normal Protocol All steps performed at room temperature Step Cycles Time Pump Speed Blocking 1 60 min Normal Wash 5 3 min Normal 1 Antibody 1 120 min Normal Wash 5 3 min...

Page 21: ...with primary antibody overnight at 4 C with shaking The following day insert membrane into eZwest and run the following method at room temperature Step Cycles Time Pump Speed Wash 5 3 min Normal 2 An...

Page 22: ...trong background Insufficient wash 1 Increase number of wash cycles set to 5 cycles for 3 minutes each 2 Increase pump flow per wash cycles set to normal Weak signal 1 Antibody is too diluted 2 Instru...

Page 23: ...lations citations handbooks etc 2 Complete technical support contact information 3 Access to the GenScript Online Catalog 4 Additional product information and special offers For more information or te...

Page 24: ...gular wash buffer Recipe 1 PBST TBST 1L 10x TBS buffer Recipe Tris base 24g NaCl 88g Dissolve in 900mL water pH to 7 4 with 12N HCl Add water to 1L 1x TBST buffer Recipe 10 TBS 100mL Tween20 0 5mL Add...

Page 25: ...23 1x PBST buffer Recipe 10 PBS 100mL Tween20 0 5mL Add water 900mL Mix thoroughly pH should be around 7 4...

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Page 28: ...GenScript USA Inc 860 Centennial Ave Piscataway NJ 08854 Tel 732 885 9188 1 877 436 7274 FAX 1 732 5185 5150 Email product genscript com...

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