GenScript eStain L1, Instructions Manual, Page: 22 / 24

- 19 -
1. The electrophoresis process
has significant impact on the
staining results. We strongly
recommend customers to use
fresh electrophoresis buffer
each time. Try to avoid PAGE
gels with high concentration of
ions.
2. Run one or more destaining
program to further destain the
gel.
3. If the PAGE gels contain high
concentration of ions or with
reused electrophoresis buffer,
please run an equilibrating
program before staining.
4. Please check the reaction
chamber after the run. If there
are lot of residual solution in
the chamber, please contact
our engineers.
Q9 Dark or unclean
background of the stained gel
Staining result
(Q9~Q12)
3. Troubleshooting
Problems
Solution Description of the problem
Q7 machine repeatedly gives
"Not enough solutions" warnings.
Other devicer
elated issues.
(Q7~Q8)
Please check the tubes to see if
there are twists and that the end
of the tubes form a seal with the
bottom of the container, If not,
please enter the "engineering
interface" to change the initiating
time
Q8 Channel A and Channel B
have different running time
even they use the same
program or parameters.
Or the time of different runs
using the same channel and
program are different.
1. Channel A and B have
independent detectors to check
the steps of the staining
process, so the running time
between the two channels may
be different.
2. The pumping time is determined
by the solution level in the
reaction chamber and will be
different between each run. The
machine automatically calculate
an estimated running time
based on the time used by the
previous run. If the actual
pumping time is shorter than the
previous run, machine will skip
the spare time and result in a
shorter running time than
estimated.