GenScript eStain L1 Instructions Manual Download Page 1 | Manualshive

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For fast coomassie blue staining of protein in mini polyacrylamide gels

Version 09302022

e

Stain

TM

L1

Protein Staining System

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Summary of Contents for eStain L1

Page 1: ...For fast coomassie blue staining of protein in mini polyacrylamide gels Version 09302022 eStainTM L1 Protein Staining System...

Page 2: ......

Page 3: ...Maintenance 2 Instructions 1 2 2 1 Instrument display and feature locations 2 2 General guidelines and Buffer preparation 2 3 Using the Pre programmed Staining method 2 4 Advanced Instruction 2 4 1 Wo...

Page 4: ...upplied with the unit is compatible with local socket format see instrument specifications Keep the area around the device clear especially at the back of the instrument to ensure adequate ventilation...

Page 5: ...n material and workmanship for a period of one year from the date of purchase or an accumulative working time of 10 000 minutes Channel A B whichever comes first If any defects occur during this warra...

Page 6: ...ess the start button to start the staining process The instrument automatically pumps in out the staining destaining buffers and no additional reagents or operations are needed eStainTM L1 works with...

Page 7: ...lay alarm press key light LED Suitable for fast Coomassie blue staining of proteins in mini polyacrylamide gels ABS PP Titanium Plasticized silicone Stainless steel 15 40 Stainless steel Polycarbonate...

Page 8: ...Two Pass Screw Cap Fit 5 L and 10 L container eStain Gel Holder Tube Instrument Cover Size 5 L 10 L 3 pk 2 pk 10 m 20 m Cat NO L00661 5 L00663 L00658 L00667 Instrument Consumables List Some reagents m...

Page 9: ...a cleaning program user can also customize a program by setting the staining cycles to 4 each cycles times to 5 min and equilibrium cycle and destain cycle to 0 4 Insert the staining solution inlet pi...

Page 10: ...rma Channel B lnforma The present state of channel The remainder of the current program Channel A Program Channel B Program Channel A and Gel Holder Keypad Channel B and Gel Holder Display Staining So...

Page 11: ...ber Reduce reaction time Reduce initial liquid inlet time Reduce the pipeline emptying time Long press to enter Method Interface Short press to select the highlighted program and return to Working Int...

Page 12: ...ace Press all together until beeps Channel A starts to run Destain Program Press all together until beeps Channel B starts to run Destain Program Press all together until beeps Channel A starts to run...

Page 13: ...mix well Add 2L ddH2O into step 3 bottle and mix well 0 2L 3L 5L 1 2 3 4 Step Total Volume Volume Prepare a clean 5L empty bottle Add 1L M00707 Concentrated Destaining Solution into 5L bottle Add 4L...

Page 14: ...the gel cassette and briefly rinse the gel with distilled water for 1 min Notice If you re using precast gels from Life Technology please remove the upper thicker part of the gel beneath the comb bef...

Page 15: ...el to start the program The timer of the corresponding channel starts to countdown 10 The channel stops flashing and the screen returns to normal Gel ExpressPlus PAGE Gel 4 12 10 wells Genscript M4121...

Page 16: ...oli cell lysis 2 PAGE MASTER Protein Standard Plus 5 l GenScript MM1397 500 3 PAGE MASTER Protein Standard Plus 2 5 l GenScript MM1397 500 5 BSA 100 ng 4 BSA 200 ng 7 BSA 25 ng 6 BSA 50 ng 9 BSA 6 25...

Page 17: ...s Description Meanings Working Stops Waiting Finished Channel Emptying Waiting for Channel to empty the pipeline insufficient Solution and Waiting Pipeline Emptying The channel program is running The...

Page 18: ...g Destaining Equilibrium Custom methods Applicable to most gels Press all together until beeps Channel A runs Destain Program Press all together until beeps Channel B runs Destain Program Press all to...

Page 19: ...ium cycles staining cycles and destaining cycles In this interface you can set the number of cycles for the equilibrium staining and destaining program and the duration for each cycle Note Cycle numbe...

Page 20: ...the process If the red part in the display panel is Channel B press to continue the process 17 Problems Solution Description of the problem Q1 Q2 Q3 starting up Q1 Q2 During Operation Q3 Q6 The instru...

Page 21: ...e the process If the red part in the display panel is Channel B press to continue the process Problems Solution Description of the problem 18 Q4 Q5 Q6 Each channel has its own current overload protect...

Page 22: ...arnings Other devicer elated issues Q7 Q8 Please check the tubes to see if there are twists and that the end of the tubes form a seal with the bottom of the container If not please enter the engineeri...

Page 23: ...em Q10 Spots on the gel 1 Air bubbles between the gel and filter paper might occasionally cause such problems run an additional destaining program to remove the spots 2 Edge effect sometimes will also...

Page 24: ...GenScript USA Inc 860 Centennial Ave Piscataway NJ 08854 Tel 732 885 9188 732 885 9688 Fax 732 210 0262 732 885 5878 Email product genscript com...

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