- 22 -
e
Stain
®
2.0 Protein Staining System
www.genscript.com
- 21 -
e
Stain
®
2.0 Protein Staining System
www.genscript.com
Examples of Results
7
μ
l New England Biolabs
®
Protein Ladder (P7703S) were loaded in each lane
and separated on 4-20% Express PAGE Gel (Bis-Tris gel). The gel was then
stained using
e
Stain
®
2.0 Protein Staining System with CBB R-250.
Samples were separated on a 12% Tris-Glycine gel and stained using
e
Stain
®
2.0
Protein Staining System with CBB R-250.
Tris-Glycine Gel
Results
Bis-Tris Gel
Results
Lane 1
1
μ
g mouse IgG
Lane 2
200 ng recombinant His-tagged protein
Lane 3
10
μ
l
E.coli
lysate
Lane 4
5
μ
l
E.coli
lysate
Lane 5
400 ng reduced BSA
Lane 6
100 ng reduced BSA
Lane 7
50 ng reduced BSA
Lane 8
20 ng reduced BSA
Lane 9
10
μ
l New England Biolabs
®
Protein
Ladder (Cat. No. P7703S)
Lane 10
5
μ
l New England Biolabs
®
Protein
Ladder (Cat. No. P7703S)
1
2 3
4 5 6
7
8 9 10
Troubleshooting,
continued
Problem
Ring-like spots observed on
the protein bands.
Cause
1. Air bubbles trapped in the
assembled staining stack.
2. Small gel pieces attached on
the surface of the gel.
Solution
1. Use the small shovel
supplied with
e
Stain
®
2.0
Device to press air bubbles
out after staining stack
assembly.
2. Make sure to remove all the
gel pieces by washing the
gel in distilled water prior to
staining.
It is hard to close the lid or the
lid can't be closed tightly
1. The lid is not closed properly.
2. Extra Sponge Cushions
are placed in the anode tank.
1. Follow the instruction on
page 14 to properly
close the lid.
2. Use 1 piece of Sponge
Cushion when staining
1.5 mm gel; use 2 pieces of
Sponge Cushion when
staining 0.75 and 1mm
gel.
