5.4.3
Flow cell path length
Description
A Flow cell path length test is used to derive the actual path length of the UV flow cell.
The test should be performed when the UV Monitor or the UV flow cell has been replaced,
and when normalized UV absorption comparisons between different systems are
needed.
Parameter description
Description
Parameter
Path length of the UV flow cell (mm).
Cell
The absorbance of the liquid in the UV flow cell (mAU).
Abs
Ambient temperature compensation constant.
C amb
UV LED drift compensation constant.
C drft
NOTICE
UV Configuration of C amb and C drft values must be performed
by a GE Service Engineer. The C amb and C drft values should not
be altered by a user.
Prerequisites
•
Buffer A: immerse Buffer A inlet into demineralized water.
•
Buffer B: freshly prepared 1.0% acetone solution (vol/vol), which is expected to give
an absorbance value of 340 mAU.
ÄKTA start Maintenance Manual 29-0603-08 AC
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5 Service and settings
5.4 UV Monitor
5.4.3 Flow cell path length
