GE HEALTHCARE Biacore T100, Handbook

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Biacore T100 Software Handbook BR-1006-48 Edition AE
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Evaluation software – general features 6
Solvent correction is applied only to response levels during sample injection,
since the correction adjusts for differences in the bulk refractive index of the
samples. Before and after sample injection, when buffer is flowing over the
surface, the bulk response is constant and correction is meaningless.
6.7.4 Applying solvent correction
To apply solvent correction, choose Add solvent correction from the Evaluation
menu. In order to apply solvent correction, the run must include solvent
correction cycles (see Section 5.6.1).
The left-hand panel of this dialog lists the solvent correction curves in the run,
and the corresponding curves are shown in the right-hand panel. For each
solvent correction cycle, there is one correction curve for each set of reference-
subtracted sensorgrams. All cycles are shown by default in an overlay plot.
Select specific rows in the cycle list to display the corresponding solvent
correction curves. Clear the checkmark in the
Included box to exclude cycles
from the correction calculation. You must include at least one solvent correction
cycle for each curve. Sample responses are corrected according to the curve
obtained from the nearest preceding correction cycle in the run. If there is no
preceding correction cycle, the nearest following cycle is used.
Examine the curves for fitting to the experimental points. Right-click on outlying
points to exclude either the single point from the curve fit or the whole
correction cycle from the correction process. Statistical fitting parameters (chi-
squared) are shown for each correction curve in the cycles list. Right-click on a
point or curve in the right panel and choose
Show sensorgrams if you want to