11-0027-48 AC, 2007-09 • p2
System Preparation
1
Starting the system
a) Press the button on the back of the system.
b) Wait until the system self-test has been completed
(30-40 seconds).
Templates
is displayed when the
system is ready to use.
2
Starting the computer
a) Start the computer.
b) Start PrimeView™.
c) Check the communication between system and
computer.
Tip! If communication has been established, the
text
Controlled By: prime
should be displayed at
the lower right corner of the screen. If not, check the
connections.
3
Purging the system flow path
a)
If the flow path is filled with storage solution (e.g.
ethanol 20%), remove it as follows:
• Place the three brown waste tubings in waste.
• Set all inlet tubings (B, A1, …) that will be used in
water (see the appropriate cue card).
• Select the application template
System Wash
Method
, select the inlets to be washed. Press
OK
.
Note: Wait at least 15 minutes before using the
system after the lamp has been turned on to avoid
drifting UV base line.
c)
If using pH:
Calibrate the pH. The pH must be calibrated every day.
See
ÄKTAprime plus User Manual
for instructions.
5
Checking the flow rate
a) Set the injection valve to position WASTE.
b) Start the flow (1-50 ml/min).
c) Use a graduated glass cylinder to collect at the WASTE
outlet (port 5). Collect during at least one minute.
Note:
Inaccurate flow rate may be due to air in the
pump. If this is the case, flush the pump with buffer
and try again. If the problem persists, flush the system
with ehtanol or methanol followed by water.
System Wash Method
Select Buffer V. Pos
B,A:_,_,_,_,_,_,_,_ OK
Application Template
Templates
b)
If there are large amounts of air in the tubing:
Fill the tubing using Purge kit P-950. See
ÄKTAprime™
plus User Manual
for detailed instructions.
4
Preparing the monitors
a) Check the UV lamp filter position and the lamp
position.
b) Check that the UV lamp is on.
Lamp
(on)
Set Parameters
Setup and
Calibration
Set Delay UV to Frac
(x µl)
Set Parameters
6
Preparing the fraction collector
a) Check that the delay volume is correct. The delay
volume is equal to the volume between the UV flow
cell and the fraction collector (default 380 µl).
d)
Check that the sensor is in the
correct position for the tube
size. The eluent tubing should be
positioned above the center of the
collection tube. Use the red sensor
control to position the tube holder.
b) Fill the fraction collector
with tubes
*
.
c) Adjust the height of the
delivery arm so that the
horizontal mark on the
tube sensor is at the same
level as the top of the
collection tubes.
Lock knob
5 mm
Sensor Control
*
See each cue card for information on the number of tubes to be
used.
Summary of Contents for AKTAprime plus
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