Gambro COBE 2991, Essentials Manual

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6-38 COBE 2991 Cell Processor • Essentials Guide
Processing and Common Operations
2
Open the centrifuge cover interlock latch (if applicable), open the sliding
covers, and remove the centrifuge bowl cover and alignment blocks.
3
Install a cell processing set, but do not connect any fluids. See “Installing the
Cell Processing Set” on page 6-2.
4
Replace the alignment blocks and the centrifuge bowl cover.
5
Close the sliding covers and the centrifuge cover interlock latch (if applicable).
Verify that the READY light is illuminated.
6
In rapid succession, press the STOP RESET, START SPIN, and SUPER OUT
buttons.
The hydraulic pump will inflate the flexible diaphragm.
7
When the AGITATE WASH IN light comes on, press the STOP RESET
button.
8
Open the centrifuge cover interlock latch (if applicable), open the sliding
covers, and remove the centrifuge bowl cover and alignment blocks.
9
Press down on the processing set in the centrifuge bowl (Figure 6-24) and
manually force out as much liquid as possible from under the diaphragm until
it lies flat in the centrifuge bowl. Be careful not to puncture the diaphragm
with jewelry, fingernails, or other sharp objects.
Figure 6-24: Manually priming
10
Replace the alignment blocks and the centrifuge bowl cover.
11
Close the sliding covers, and then close the centrifuge cover interlock latch (if
applicable). Verify that the READY light is illuminated.
Note: There is no
way to check that
the COBE 2991 is
primed without the priming
cushion and disk.
12
To ensure that the hydraulic system is primed, repeat steps 6 through 11 as
necessary.
13
Set the PUMP RESTORE RATE to its previous setting, otherwise the COBE
2991 will not advance to the next cycle.