Gambro COBE 2991, Essentials Manual

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6-14 COBE 2991 Cell Processor • Essentials Guide
Processing and Common Operations
Loading the Starting Cell Product
The following sections provide a generic overview of connecting the starting cell
product and solutions, predilution, and loading. For instructions regarding specific
protocols, refer to the
COBE 2991 Cell Processor Protocols Guide .
Connecting the Starting Cell Product and Solutions
The starting cell product is typically connected to the red-striped tubing (through
valve V1), with processing/wash solutions connected to the green and yellow-
striped tubing (through valves V2 and V3, respectively). This configuration
(Figure 6-13) allows the use of the Blood-In and Air-Out operations to load the
starting cell product and remove trapped air from the cell processing bag. You have
the option of connecting additional processing/wash solution or a collection
container to the blue-striped tubing.
Note: For
connecting and
loading instructions
regarding specific protocols,
refer to the
COBE 2991 Cell
Processor Protocols Guide .
Figure 6-13: Typical starting cell product and processing/wash solution connections
If you are using the red-striped tubing to load the starting cell product, use the
following procedure:
1
Remove the cap from the spike on the green-striped tubing, and insert the
spike into a processing/wash solution container. Hang the container on the
right hanger bar.
2
If additional processing/wash solution is required, remove the cap from the
spike on the yellow-striped tubing, and insert the spike into a processing/wash
solution container. Hang the container on the right hanger bar.
3
Remove the cap from the spike on the red-striped tubing, and insert the spike
into the starting cell product container. Hang the container on the left hanger
bar.
4
Close the slide clamp on the blue-striped tubing.
If you are using the blue-striped tubing, remove the cap from the spike on the
blue-striped tubing and insert the spike into a collection container or
processing/wash solution container. Hang additional processing/wash solution
on the left hanger bar.
Caution:
Use
aseptic technique
while connecting
cell product and
processing/wash solutions
to the cell processing set.
Starting cell product
Processing/wash solution(s)
Additional
processing/wash
solution or collection
container (optional)