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FujiFilm FLA-7000 Operation Manual Download Page 25

Part

4

Reading Digitized Samples

Summary of Contents for FLA-7000

Page 1: ...Version 2 0 2006 Mar Operation Manual...

Page 2: ...2006 Mar...

Page 3: ...he FLUOR Stage on the FLA 7000 25 4 Starting Reading 25 Part 5 Lasers and Filters Other Settings 29 1 Registering Laser and Filter Combinations Method 30 2 Filter Module Settings Filter Module 31 2a R...

Page 4: ...ii 2006 Mar 3 Installation For MacintoshTM 50 4 Uninstallation For MacintoshTM 50 Part 7 Troubleshooting 51 1 Errors 52 2 Warnings 52...

Page 5: ...Part 1 Preparations Before Use...

Page 6: ...fessional SP2 or later Mac OS X 10 3 or later Memory 512 MB Interface USB 2 0 Precautions for Use Do not connect any USB devices other than the FLA 7000 to the computer in which the Image Reader is in...

Page 7: ...use the shortcut key For the Macintosh version Double click the alias or the software to start the Image Reader FLA 7000 software Note After starting the Image Reader its condition is displayed in the...

Page 8: ...ready for scanning Filter Module Use this button to change or register the filter Preference Use this button to set the display menu file format or the Log Square Root of image data type Filter The l...

Page 9: ...Part 2 Reading Imaging Plates...

Page 10: ...ke these settings before reading IPs Refer to the following explanations of reading conditions when making settings Specify where to save the file after reading Click the button Input the name of a fi...

Page 11: ...ons are not fulfilled the Reader Settings window for the IP mode cannot be accessed Click to select from three types of reading sensitivity as shown on the left Set the pixel size for reading Click to...

Page 12: ...aving the reading conditions in a file You may save reading conditions that are used frequently with this function and recall them later with Use this button when recalling reading conditions saved wi...

Page 13: ...ding may be finished at any time before the whole scanning area has completed reading Click the button when you want to finish reading Note1 If you click Stop during reading the part that has not been...

Page 14: ...be displayed as a completely white image and data of a higher light intensity than the line on the right High value will be displayed as a completely black image If Auto Range Scope is checked the Im...

Page 15: ...procedures Click the button to return to the first Reader Settings window Do not open the stage door of the FLA 7000 until the stage has completely returned If it is opened close it immediately When s...

Page 16: ...lkali PMT is done by a serviceman For details contact an authorized dealer The Sensitivity settings differ from that of the regular IP mode The operation procedures for other settings are the same as...

Page 17: ...Part 3 Reading Fluorescent Samples...

Page 18: ...reading fluorescent samples Refer to the following explanations of reading conditions when making settings Click the button and specify where to save the file after reading Note When scanning multipl...

Page 19: ...d with the image as a file and can be viewed with the analyzing software Input it if necessary Scanning can be performed up to 4 times The following items can be set Note From the pull down menu selec...

Page 20: ...ected is bigger with L5 than with L4 If the signals of the sample are in the L4 range the density gradation is represented more finely if L4 is selected Estimated time until reading finishes Size of t...

Page 21: ...e previous session is displayed Set the fluorescent sample on the FLUOR stage For instructions on setting the fluorescent sample see the Fluorescent Image Analyzing System FLA 7000 Operation Manual Se...

Page 22: ...ill be saved as an image with a data value of 0 light intensity of 0 Note2 When you click the button reading itself is canceled You cannot start reading again from the location where reading stopped 4...

Page 23: ...nes are indicated by the horizontal axis of the tone curve graph You may change the display area by selecting a magnification ratio from the pull down menu In addition after reading if you click the d...

Page 24: ...20 2006 Mar 4 5 Finish reading Before turning off the power of the FLA 7000 shut down the Image Reader software 4 Starting Reading...

Page 25: ...Part 4 Reading Digitized Samples...

Page 26: ...re reading digitized samples Refer to the following explanations of reading conditions when making settings Specify where to save the file after reading Click the button and specify where to save the...

Page 27: ...ions are not fulfilled the Reader Condition Settings window for the Digitizing mode cannot be accessed You may set the voltage to be applied to the photo multipliers PMT as an integral value within th...

Page 28: ...ge Select this to specify the reading area arbitrarily Select this to set the entire FLUOR stage as the reading area Use this button when saving the reading conditions in a file You may save reading c...

Page 29: ...yzing System FLA 7000 Operation Manual Set the FLUOR stage on the FLA 7000 For instructions on setting the FLUOR stage on the FLA 7000 see the Fluorescent Image Analyzing System FLA 7000 Operation Man...

Page 30: ...make settings Select the type of tone curve from the pull down menu Exponential The exponential tone curve is used to adjust gradations Linear The linear tone curve is used to adjust gradations Sigmo...

Page 31: ...icked area can be enlarged or reduced 4 3 Save the data with a different file name Launch the registered analyzing software to display the image 4 4 To read another digitized sample continuously carry...

Page 32: ...28 2006 Mar...

Page 33: ...Part 5 Lasers and Filters Other Settings...

Page 34: ...Click this button to register new laser and filter combinations Click this button to edit laser and filter combinations Click this button to delete laser and filter combinations 1 2 Click the button...

Page 35: ...rviceman upon installation Under normal circumstances it is not necessary to register these settings After exchanging the filters of the FLA 7000 you must register the exchanged filters in the Image R...

Page 36: ...000 Operation Manual Use this button to set a filter in the Image Reader It is also possible to use the mouse to drag and drop Use this button to remove a filter in the Image Reader Corresponds to the...

Page 37: ...s around the selected filter position 2a 4 Select 605DF40 from the Filter List The selected item is highlighted in blue 2a 5 Click the button It is also possible to drag and drop the selected filter t...

Page 38: ...g recalling filter combinations can be managed more easily if each user has their own filter module 2b 1 Click the button on the main window 2b 2 Click the button The following dialog box appears Filt...

Page 39: ...ant to recall and click the button The filter combination is recalled 2c 4 Click the button You may register a filter name 2d 1 Click the button on the main window 2d 2 Click the button The following...

Page 40: ...nitially registered as default cannot be deleted or edited 2d 4 Click the button These are settings that are carried out when specifying settings for reading samples Depending on the reading mode ther...

Page 41: ...for quantitative analysis of images read in the Root format Root format files cannot be opened properly with older software versions For Windows Science Lab 2003 Multi Gauge Ver 2 1 Colony Ver 1 1 L...

Page 42: ...210sec 50um 330sec 25um 450sec Quick Mode 200um 150sec 100um 150sec 50um 210sec 25um 330sec 3a 4 A function for supporting new stages in the future 3a 5 Click the button These are settings for saving...

Page 43: ...information file inf file and a raster file img file The Fuji Film Science Lab or Array Gauge may be used for analysis In combination with an img file and inf file a read image can also be saved in T...

Page 44: ...40 2006 Mar...

Page 45: ...Part 6 Installing and Uninstalling the Software...

Page 46: ...Installation of FUJI USB Control driver 1b Installation of FUJI USB Function driver 1c Installation of Image Reader FLA 7000 software Note The computer and FLA 7000 should not be connected with a USB...

Page 47: ...4 Select Yes I have already connected the hardware and click the Next button 1a 5 Select Add a new hardware device and click the Next button 1a 6 Select Install the hardware that manually select from...

Page 48: ...6 Mar 1a 7 Select Show All Devices and click the Next button 1a 8 Click Have Disk button 1a 9 Click Browse button 1a 10 Set the folder in which a file is saved to FLA 7000 CD ROM 1 Installation For Wi...

Page 49: ...45 2006 Mar 1a 11 Open the FUJIUSB control folder 1a 12 Select a DevMng inf file and click the Open button 1a 13 Click the OK button 1a 14 Click the Next button 1 Installation For Windows...

Page 50: ...46 2006 Mar 1a 15 Click the Next button 1a 16 Click the Continue Anyway button 1a 17 Click the Finish button 1 Installation For Windows...

Page 51: ...and turn ON the power switch of the FLA 7000 Note Perform this operation or the personal computer may be reset 1b 2 Click the Next button 1b 3 Click the Finish button 1b Installation of FUJI USB Func...

Page 52: ...the installation CD ROM of FLA 7000 1c 2 Click the setup icon 1c 3 Click the Next button 1c 4 Click the Install button 1c 5 Click the Finish button 1c Installation of Image Reader FLA 7000 Software 1...

Page 53: ...played and uninstallation is started 2 Uninstallation For Windows Note Connection files created after installation of the reader software are deleted Correction files such as shading data is required...

Page 54: ...n the Installation Type screen 3 6 Provide the name and password of the administrator 3 7 Click the close button on the Finish Up screen 4 Uninstallation For MacintoshTM Move the FLA 7000 folder conta...

Page 55: ...Part 7 Troubleshooting...

Page 56: ...ge includes instructions please follow them Message Filter module has not been setup Push the filter module button and set up the filter module The combination of the lasers and filters could be inapp...

Page 57: ...n using a different laser Meaning There was a failure in reading the image data from the computer Check the computer environment Countermeasure It is possible that the computer s processing ability ha...

Page 58: ...uble Please contact the dealer where you purchased the FLA 7000 or contact Fuji Photo Film Fuji Photo Film Co Ltd 2 26 30 Nishi Azabu Minato ku Tokyo Japan 106 8620 TEL 81 3 3406 2201 FAX 81 3 3406 21...

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