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FLUIGENT
Siège social : Biopark
–
1 mail du Professeur Georges Mathé
–
94800 Villejuif
–
France
Tel : +331 77 01 82 68
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Fax : +331 77 01 82 70
www.fluigent.com
SA à Directoire et Conseil de Surveillance au capital de 141 284.95
€
- Siret : 487 636 409 00038
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N°TVA UE/EU VAT Number : FR 53 487 636 409
Make a table that contains the time for each measurement, the flow-rate of the pump and the data measured
by the FLOW UNIT. A minimum of 3 measurements is recommended for each flow-rate.
The principle of the experiment is to inject the FC-40 through the desired FLOW UNIT model connected to the
FLOWBOARD. Then simultaneously you record the flow-rate given by the software and you measure the weight
of fluid you have collected over a chosen period of time. Knowing the density of the fluid, you are able to
define the actual flow-rate.
Note that if a peristaltic or a syringe pump is used, one has to wait until the target flow-rate is reached (settling
times can be long) and to calculate an average flow-rate due to the pulsations.
The list of materials needed to reproduce the experiment is given below:
- One (1) FLOWBOARD
- One (1) FLOW UNIT model
- One (1)
MFC“™
-EZ or with the appropriate pressure range (1 bar for FC-
a d Maesflo™ .
software (or later versions)
- One (1) precision weighing scale
The table below displays the information recorded during the experiment: the pressure imposed by the
MFC“™
-EZ, Qs the flow-rate recorded by the FLOW UNIT through the Flow-Rate Platform software, Qw the
flow-rate measured with the precision weighing scale, and Qw/Qs the calculated scale factor for a single point
calibration.
Pressure (mbar)
Q
S
(µl/min)
Q
w
(µl/min)
Q
w
/Q
S
596.3
91.6
317.8
3.5
Consequently, when working around 317 µl/min (target flow-rate), you have to add the scale factor of 3.5 so
that the measurement of the sensor corresponds to the actual flow-rate for FC-40.
