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accumet AE150 pH Benchtop Meter 

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mV Calibration  

If relative mV has never been calibrated or if the meter has been reset, the value shown in 
the upper display during the calibration will be the same as the absolute mV value.  

The range of adjustment is -1999 mV to +1999 mV with 1 mV resolution or -199.9 mV to 
+199.9 mV with 0.1 mV resolution.  

1.  To power the meter on, press the 

ON/OFF

 key. All the 

display segments will be displayed briefly and then the pH 
measurement mode will be displayed. 

a.  If necessary press the 

MODE

 key to select the pH 

measurement mode.  

2.  Press the 

CAL/MEAS

 key to enter the calibration mode. 

a.  The “CAL” icon will be shown on top of the display to 

indicate the meter is in R.mV calibration mode.  

b.  The upper display will show the relative mV reading 

and lower display will indicate the absolute mV value.  

3.  Rinse the electrode with an appropriate rinse solution. If 

using a separate temperature probe, thoroughly rinse the 
temperature probe as well.  

4.  Select a standard and pour the standard into a clean 

container.  

5.  Immerse the electrode and temperature probe into the 

standard.  

6.  Press the 

 or 

 arrow key to set the relative mV value to match the desired 

standard value.  

7.  Press the 

ENTER

 key to confirm the reading and the meter will proceed to the 

measurement mode. The upper display will show the relative mV reading and the 
“R.mV” icon will appear in the upper right hand corner.  

Note: 

New mV calibrations will override existing stored mV calibration data. The meter 

retains stored mV calibrations even when the meter is turned off. 

 

 

 

 

Summary of Contents for accumet AE150

Page 1: ...et AE150 pH benchtop meter Detailed information on meter setup operation and features is provided in the appropriate sections of this user manual Please visit www fishersci com accumet for addional information on Fisher Scientific accumet meters and electrodes accumet AE150 pH Benchtop Meter Revision Date December 9 2015 68X000635 Revision A ...

Page 2: ...2 P4 0 Meter Reset Program 13 Calibration 14 pH Calibration 14 mV Calibration 17 Temperature Calibration 18 Measurements 19 Temperature Compensation 19 pH or mV Measurements 20 HOLD Function 20 Storing and Recalling Data from Memory 21 pH Electrode Maintenance 22 After Use Storage 22 Electrolyte Replacement for refillable electrodes only 22 Electrode Cleaning and Rejuvenation 23 Troubleshooting Gu...

Page 3: ...of two pH buffer sets pH slope and offset display One point mV calibration with 150 mV offset adjustment One point ATC temperature probe calibration with 5 C offset adjustment Automatic or manual temperature compensation Measure 0 00 to 14 00 pH with 0 01 pH relative accuracy Measure 1999 to 1999 mV with 0 2 mV relative accuracy 199 9 mV Measure 0 0 to 100 0 C with 0 3 C temperature relative accur...

Page 4: ...luttered display Icon Description SETUP The meter is in the setup mode MEAS The meter is in the measure mode CAL The meter is in the calibration mode MEM Data is stored to the memory READY The measured value is stable HOLD The displayed value has been frozen Prompts user to select buffer during calibration or indicates buffer error when flashing with ERR icon Indicates electrode error when flashin...

Page 5: ...the measurement mode MODE Toggles between the different measure modes available pH mV and temperature Switches from pH to temperature in pH calibration mode HOLD Freezes a measurement on the display Press HOLD key again to update display with current readings MR MR key recalls measurements from memory and scrolls through log values Down arrow key scrolls to next program in the SETUP mode Down arro...

Page 6: ...ure probe INPUT For connection to sensors with BNC connectors the meter accepts pH or ORP electrodes with a BNC connector always ensure that the connector is clean and dry REF For connection to pin type reference half cell electrode used with half cell pH electrode REC For connection to strip chart recorders use subminiature plug with positive tip to capture raw mV values GND For connection to the...

Page 7: ...ector with the posts of the socket Rotate the connector clockwise until it locks For a separate reference half cell electrode push the reference electrode pin into the meter jack marked REF Connecting the ATC Temperature Probe Insert the temperature probe into the connector marked ATC Connecting the AC Adapter Slide the jack of the AC adapter into the meter input marked DC until it is firmly seate...

Page 8: ...t lines up with the original screw hole Use the screw removed earlier to secure the electrode holder into position Note The side metal bar is reversible If desired remove screw holding electrode holder base and slide out of brackets Slide base into brackets on opposite side and tighten screw To install the electrode arm to the meter To mount the electrode arm into the metal rod on the side bar ali...

Page 9: ... C for temperature and mV for millivolt Meter Reset On first use it is not necessary to reset the meter before calibration To reset the meter to factory default refer to the Meter Setup Functions section Note After the meter is reset a re calibration is required before measurement Likewise to prepare a new set of measurements all the last calibrated pH values can be reset simultaneously for added ...

Page 10: ...L MEAS key two times before the meter reverts to the measurement mode P1 0 Clear Memory Program Use this program to clear all memory values when you need to store a new series of values This avoids confusing the old values with the new ones NO is the default setting 1 With the meter powered off press and hold the MODE key and press and release the ON OFF key The display will show the SETUP indicat...

Page 11: ... scroll through the programs until ELE is shown in the upper display and P2 0 is shown in the lower display The electrode icon will also appear 3 Press the ENTER key to access the program P2 0 The lower display will show P2 1 4 The information shown in program P2 0 will depend on the mode the meter was in prior to powering the meter off a In the pH measurement mode i P2 1 shows the mV offset of th...

Page 12: ...y to access the program P3 0 The lower display will show either USA or nSt depending on last option selected or factory default of USA 4 Press the or arrow key to select the desired calibration buffer set a USA buffers 1 68 4 01 7 00 10 01 12 45 b nSt buffers 1 68 4 01 6 86 9 18 12 45 5 To confirm the selected buffer set press the ENTER key The meter will proceed to the selection mode for the numb...

Page 13: ...e lower display will show nO 4 Press the or arrow key to select nO or YES a Selecting YES will erase all calibration values for pH and mV modes CAL or reset the meter back to the factory default conditions FCt b Selecting nO will deactivate the reset program 5 To confirm the reset make sure YES is shown and press the ENTER key 6 Press the or arrow key to select CAL or FCt as the reset function a S...

Page 14: ...libration will not be accepted For slope to slope calibration two point calibration the first calibration point will only be successfully registered in the meter after the second point calibration has been completed It is recommended that you perform at least a two point calibration using buffers that bracket the expected sample range one buffer above the sample pH and one buffer below the sample ...

Page 15: ... toggle between the next calibration buffer values based on the selected buffer set 9 Save the calibration or proceed to the next calibration point a If performing a one point calibration press the CAL MEAS key to return to the measurement mode and start taking pH readings b If performing a two to five point calibration continue to step 10 do not press the CAL MEAS key Two to Five Point Calibratio...

Page 16: ...o stabilize and the READY icon to be shown on the display 20 Press the ENTER key to confirm the calibration a The upper display will flash the calibration value briefly before storing the calibration point in the meter b The meter will automatically proceed to the measurement mode after a three point calibration is completed unless the number of calibration points is set to 4 or 5 using the P3 0 P...

Page 17: ...lay to indicate the meter is in R mV calibration mode b The upper display will show the relative mV reading and lower display will indicate the absolute mV value 3 Rinse the electrode with an appropriate rinse solution If using a separate temperature probe thoroughly rinse the temperature probe as well 4 Select a standard and pour the standard into a clean container 5 Immerse the electrode and tem...

Page 18: ...rature calibration you must be in the pH measurement mode a If necessary press the MODE key to select the pH measurement mode 3 Press the CAL MEAS key to enter the pH calibration mode 4 Press the MODE key to switch to the temperature calibration mode 5 Press the or arrow key to scroll to the correct temperature value corresponding to the known solution temperature a The meter allows a limit of 5 C...

Page 19: ...he CAL MEAS key to enter the pH calibration mode The CAL icon will be shown above the upper display 4 While in pH calibration mode press the MODE key to enter temperature compensation mode a The upper display will show the current temperature setting and the lower display shows the default value 25 0 C if the meter has never been manually set for temperature or the last manually set value if the m...

Page 20: ...3 Press the MODE key to toggle between the pH or mV measurement mode 4 Immerse the electrode and temperature probe into the sample a If using a pH electrode make sure that the glass bulb of the pH electrode is completely immersed in the sample b Gently stir the electrode to create a homogeneous sample 5 Wait for the reading to stabilize When the reading stabilizes the READY icon will be shown Reco...

Page 21: ...urn to the measurement mode 4 To store another reading press the MI key again Note When the memory is full the oldest stored data set will be erased to create space for the new data set Memory Recall Readings stored in the memory are retained even when the meter is powered off To erase all readings stored in memory use the P1 0 program in the setup mode 1 In the measurement mode press the MR key t...

Page 22: ...rotective rubber cap or container filled with storage solution provides ideal electrode storage for long periods Electrolyte Replacement for refillable electrodes only Refill the reference electrolyte when the pH electrode has been used for an extended period or if the internal electrolyte dries up To accomplish this use the following procedure 1 Remove the protective rubber cap or sleeve to expos...

Page 23: ...yewear and precautionary measures must be used when performing the rejuvenation as it involves the use of concentrated acid and alkaline Generally if the storage and maintenance procedures have been closely followed the pH electrode can be used immediately However should the electrode response become sluggish it may be possible that the glass bulb has dehydrated Immersing the electrode in storage ...

Page 24: ...ted Measurement is under range or over range Make sure electrode is connected Clean or replace electrode Recalibrate the meter Treat samples to bring within meter measuring range General Troubleshooting Problem Probable Cause Remedial Action No display Main power not switched on Switch on the power supply AC Adapter socket not inserted properly Re insert AC Adapter socket Unstable reading Insuffic...

Page 25: ...mple pH is unknown Standard pH Buffers The following table shows pH buffer values at various temperatures during calibration The table also illustrates why a calibration value may be different from the buffer value at 25 C USA pH Buffer NIST pH Buffer Temp C 1 68 4 01 7 00 10 01 12 45 Temp C 1 68 4 01 6 86 9 18 12 45 0 1 67 4 01 7 12 10 32 13 43 0 1 67 4 01 6 98 9 46 13 43 5 1 67 4 01 7 09 10 25 1...

Page 26: ... buffer recognition ABR pH Buffer Calibration Options USA pH 1 68 4 01 7 00 10 01 12 45 NIST pH 1 68 4 01 6 86 9 18 12 45 Memory 100 data sets Hold Function Yes Self diagnostic Messages Yes pH Slope Offset Display Yes Inputs BNC 2 5 mm phono plug reference pin tip ground Output Recorder 2000 mV Power Requirements AC DC 9V Adapter 110 220VAC 50 60Hz Display Dual line LCD with icons Dimensions Meter...

Page 27: ...B double junction gel pH electrode 13 620 22 ATC probe and stand 13 636 AE153 Fisher Scientific accumet AE150 pH benchtop meter 3 in 1 set with 13 620 AE6 3 in 1 single junction gel pH ATC electrode and stand 13 620 AE6 Fisher Scientific accumet AE series 3 in 1 single junction gel pH ATC electrode 13 620 108B Fisher Scientific accumet AE series single junction gel pH electrode 13 620 299B Fisher ...

Page 28: ...thout charge Out of warranty products will be repaired on a charged basis Exclusions The warranty on the meter shall not apply to defects resulting from Improper or inadequate operator maintenance Unauthorized modification or misuse Operation outside of the environment specifications of the products For technical assistance contact your Fisher Scientific representative or visit www fishersci com a...

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