2
2. Operation of reflected illumination system (LED fluorescence unit)
To get the best result when observing with fluorescence please pay attention to the following:
1. Select the fluorescence filter that matches the fluorochrome and fluorescent characteristic.
2. No autofluorescence specimen or device should be in the optical system, such as the cedar
immersion liquid wich has cyan autofluorescence.
3. Always turn off the transmitted illumination before turning on the reflected illumination
system.
2.1 Set and adjust the fluorescence unit
The most important part of the unit is the fluorescence filters assembly. There are four
fluorescence filters, Blue, Green, Violet and Ultraviolet, mounted in the turret (fig. 2) and are
indicated as B, G, V and UV on turret. There is also a bright field option and it is indicated as 0 on
the turret.
The fluorescence filters in the turret corresponds with the LED in the fluorescence illuminator (see
table 1). There is a turret (fig. 2 number 1) on top of the fluorescence illuminator and has the same
indication of B, G, V and UV as of the turret for the fluorescence filters.
Fluorescence filters
LED light source
B
B
G
G
V
V
UV
UV
When selecting the filter, turn the turret (fig. 2 number 2) to the correct filter and choose the
corresponding LED (fig. 2 number 1) by turning the turret.
Fig. 2
Remark: For bright field observation please choose position 0 on the turret (fig. 2 number 2) and
switch off the fluorescence illuminator and turn on the transmitted illumination.
