
18
ARAMUS STANDARD BAG ASSEMBLIES
User Manual | Rev. B 03/20 | Entegris, Inc.
Step 3: Close Cassette
Close tab and then swivel locking bar into a closed
position. There should be little to no resistance from
the bag when closing. Ensure the cassette does not
bulge once closed.
NOTE: If the cassette bulges when closed, go back to
step 2 and reposition the components.
Step 4: Freezing and Storage
For ultra-low temperatures, the cassette assembly
can be placed directly into static and blast freezers
in a horizontal orientation (flat on shelf).
For freezing in a liquid nitrogen Dewar, a rack is
recommended. Be sure to slide the cassette com-
pletely inside the rack, and lock if applicable, before
immersing in liquid nitrogen vapor.
CAUTION! Always use cryogenic gloves when
handling cassettes or racks in low temperatures.
Step 5: Thawing
Inspect the cassette assembly to ensure no damage
or significant bulging occurred during freezing. The
locking bar and tab should be secure.
To thaw, remove the bag from the cassette assem-
bly and store at ambient temperature on a bench
or table. Other methods are available to accelerate
thawing such as using a fan or immersing the bag
in a water bath.
Locking bar