Endress+Hauser Analytik Jena CyBio Well vario User Manual Download Page 139

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Rev B 2022/03

123

CyBio Well vario

A 1.6Operation

A 1.6.1Operating Modes

The following operating modes are available when working with a CyBio Well vario system:



Working with manual control unit (

→

refer to chapter 7.2

Manual mode

Configuration

Statistics



PC-controlled operation (refer to Plugin Pipettor Software ManualCyBio Composer)

A 1.6.2PC Mode

PC interface

Data transfers between the CyBio Well vario, stackers, additional modules and the control

computer are accomplished via a serial RS-232C interface.



Characters are of uneven parity, each consisting of 8 data bits and 2 stop bits. This data

format cannot be changed.



The baud rate is fixed at 19200 Baud. It cannot be changed.

Fig. 129: CyBio Well vario connection to a 9- or a 25-pole RS232-PC jack

Summary of Contents for Analytik Jena CyBio Well vario

Page 1: ...User Manual CyBio Well vario ...

Page 2: ...s documentation may be duplicated photocopied saved to a storage system or transferred to electronic media without prior written permission of the publisher Publisher Analytik Jena GmbH Konrad Zuse Straße 1 07745 Jena Germany Germany Tel 49 3641 77 70 Fax 49 3641 77 9279 Service support Tel 49 3641 77 9449 E Mail service lha analytik jena com Ordering code number OL3381 25 300 BLE Document type Tr...

Page 3: ...Rev B 2022 03 III CyBio Well vario ...

Page 4: ...n conformity with the EC Directive relating to electromagnetic compatibility 2014 30 EU The safety objectives of the Low Voltage Directive are taken into account Harmonized Standards used EN ISO 12100 Safety of Machinery General principles for design Risk assessment and risk reduction EN 61010 1 Safety requirements for electrical equipment for measurement control and laboratory use EN 61000 6 2 EM...

Page 5: ...Rev B 2022 03 V CyBio Well vario ...

Page 6: ...VI Rev B 2022 03 CyBio Well vario ...

Page 7: ...Rev B 2022 03 VII CyBio Well vario ...

Page 8: ...VIII Rev B 2022 03 CyBio Well vario ...

Page 9: ... Protection Fire Prevention 19 3 10 4 Electrics 19 3 10 5 Maintenance Care 20 3 10 6 Handling of Dangerous Substances 20 3 10 7 Chemical Resistance 21 3 11 Rules of Conduct in Cases of Emergency 22 4 Technical Description 23 4 1 System Setup 23 4 2 Component Description 25 4 2 1 Basic Unit 25 4 2 2 Operation control panel 26 4 2 3 Pipetting heads 27 4 2 4 Pipetting head CyBio Well vario 1536 8 μl ...

Page 10: ... Configuration 74 7 2 4 Menu Head change 78 7 2 5 Special functions 88 7 3 Switching the CyBio Well vario off 90 7 4 Specific System Details Pipetting Head 91 7 4 1 Aspiration With Additional Stroke 91 7 4 2 Aspiration without additional stroke 92 7 4 3 Volume Cycle 93 7 4 4 Waiting Time 93 7 5 System Precision Pipetting Head 94 8 Troubleshooting 95 8 1 Error Messages 95 8 2 Errors By Compressed A...

Page 11: ...112 12 Waste Disposal 115 12 1 Consumables 115 12 2 Reagents 115 12 3 System Accessories 115 A 1 CyBio Well vario System 117 A 1 1 Overview 117 A 1 2 Technical Specifications 118 A 1 3 Safety Notes 119 A 1 4 Device Versions 120 A 1 5 Installation Startup Procedures 121 A 1 6 Operation 123 A 1 7 Maintenance Care 124 A 1 8 Troubleshooting 124 A 2 Scripts 127 ...

Page 12: ...XII Rev B 2022 03 CyBio Well vario ...

Page 13: ... 38 Fig 25 Peristaltic pump closed and open 38 Fig 26 Insertion of Tube into Peristaltic Pump 39 Fig 27 Peristaltic pump on TWS module direction of rotation and delivery of liquid 39 Fig 28 Typical behavior of the residual liquid in normal pipette tips when resetting the pistons to the zero position 40 Fig 29 Typical behavior of the residual liquid in the case of small pipette tips and capillaries...

Page 14: ...ip change magazine removal 72 Fig 80 Capillary magazine change version head replacement from the front 72 Fig 81 Display tip change magazine insertion 73 Fig 82 Display tip change tightening of tips 73 Fig 83 Display configuration menu pipetting head 74 Fig 84 A1 well target positions for 96 384 1536 well microplate 75 Fig 85 Display XY correction values 75 Fig 86 Display configuration menu capill...

Page 15: ...n with additional stroke 91 Fig 118 Aspiration without additional stroke 92 Fig 119 Toolset 106 Fig 120 Compressed air control unit condensate drain outlet 108 Fig 121 Disconnectible air supply inlet 109 Fig 122 CyBio Well vario system with two stackers 117 Fig 123 Warning notes and safety devices 119 Fig 124 Danger zones of CyBio Well vario system 119 Fig 125 Setup versions on base frame 120 Fig ...

Page 16: ...XVI Rev B 2022 03 CyBio Well vario ...

Page 17: ...ngs as bullet points Safety notes are marked with a pictogram and the appropriate signal word refer to sec tion Safety Notes for the meaning of pictograms and signal words used herein They specify the type source and consequences of a hazard giving advice for accident preven tion A safety note always precedes instructions on required action Software components for control and evaluation are highli...

Page 18: ... all operating requirements quoted in this User Manual and described procedural sequences and related safety notes are duly observed all specifications in this Manual regarding system start up operation preventive main tenance and care are met applicable safety standards or rules are always fulfilled Any type of mode of application other than specified herein will be regarded as non con forming us...

Page 19: ...services or replacement of damaged parts It will exclude consequential damage of any kind Damage to wearing parts is not included in the war ranty Any deviation from conforming use as defined in this User Manual operating require ments process sequences will result in restricted acceptance of warranty or liability claims in the event of damage In the event of personal injury or material damage cla...

Page 20: ...ery CyBio Well vario system delivery includes CyBio Well vario Transporting unit linear type or circular type Pipetting head or capillary head Compressed air control unit only for working with capillary head CyBio Composer software Toolset User manual Optional accessories ...

Page 21: ...porting unit 1650 x 772 5 x 370 mm Width x height x depth with 10 place circular transporting unit 700 x 772 5 x 895 mm Weight Basic unit without pipetting head 31 0 kg Pipetting head 14 0 kg Capillary head 10 0 kg Operation control panel 1 8 kg Basic unit with carriage and frame 39 5 kg Basic unit with rotary stage and frame 49 0 kg Pipetting heads Channels 96 384 or 1536 Volume ranges working ra...

Page 22: ...efficient of variation 2 Precision for volume range of 5 25 μL Coefficient of variation 1 Dosing volume of 96 piston pipetting head 2 5 μL Randomly selectable in steps of 0 001 μl Precision for volume range of 0 1 0 25 μl Coefficient of variation 7 Precision for volume range of 0 25 0 5 μl Coefficient of variation 5 Precision for volume range of 0 5 2 5 μl Coefficient of variation 3 Dosing volume ...

Page 23: ... 0 1 0 25 μl Coefficient of variation 7 Precision for volume range of 0 25 0 5 μl Coefficient of variation 5 Precision for volume range of 0 5 2 5 μl Coefficient of variation 3 Dosing volume of 1536 piston 8 μl Randomly selectable in steps of 0 01 μl Precision for volume range of 1 5 μl Coefficient of variation 3 Precision for volume range of 5 8 μl Coefficient of variation 2 ...

Page 24: ...0 for all volume ranges measurement of absor bance Typical values 250 nl VC 1 5 100 nl VC 2 5 25 nl VC 8 0 Pipette tips Single use tips 250 DW 250 SW 60 μl 25 μl 10 μl polypropylene PP single use tips in standard version ster ile PCR certified or APR compatible quality version Ceramic tips 2 5 μl ceramic tips Channels 96 or 384 Microplate specifications Formats SBS standard 96 384 1536 shallow wel...

Page 25: ...0 60 Hz Operating pressure only capillary head 1 72 bar 0 172 MPa 25 psi Protective fusing 230 V T1 6 A 115 V T3 15 A Power consumption 200 VA Overvoltage category II Degree of contamination 2 Interface RS 232 C Sub D 9 pin Current firmware version 4 731 Storage Operating Conditions Storage and transport Allowable ambient temperature Allowable relative air humidity 10 C to 50 C 85 at 30 C Operatio...

Page 26: ...10 Rev B 2022 03 Technical Specifications CyBio Well vario ...

Page 27: ...tection must be observed and complied with Guidelines for China The device contains substances subject to regulation according to RoHS regulation SJ T 11363 2011 3 3 NRTL Certification TIP For your own safety and to ensure failsafe and reliable CyBio Well vario system opera tion you should carefully read this chapter before proceeding to any kind of start up work You are required to follow all saf...

Page 28: ...potentially hazardous situation which if not prevented can result in death or most serious injuries incapacitation CAUTION Indicates a potentially hazardous situation which if not prevented can result in light or minor injuries and material damage TIP Provides operator advice and other particularly useful information for situations involv ing no danger or harm ENVIRONMENTAL PROTECTION Provides ope...

Page 29: ...ing accidents Consider the pipetting head s weight for installa tion or deinstallation work The capillary head weighs 10 kg 22 lbs Warns of compressed air only capillary head Make sure that the maxi mum specified pressure is not exceeded or Indicates factory setting for permissible under national standard sup ply voltage level Connection to supply volt age of a different level may result in the de...

Page 30: ...Safety Notes CyBio Well vario Fig 1 Safety labeling at pipetting head and capillary head 1 Warning note Weight 2 Warning note Magazine adapter Fig 2 Safety labeling at compressed air control unit only capillary head 1 2 ...

Page 31: ...Safety labeling provided at the system 3 6 Mechanical Danger Zones Fig 4 Danger zones at pipettor 1 Magazine clamping device 2 Lifter 3 Head cover Rearside view of system on both sides in head area with top cover open Head cover on system front side 1 2 3 ...

Page 32: ...stacker operation Danger zones are Magazine clamping device Lifter Head cover Carriage with transport rails or rotary stage Faulty action or misoperation may cause material damage or physical injury Always use software tools to correct potentially faulty microplate motion or switch power at the main switch off before you begin any kind of intervention To abort a running program you may press the c...

Page 33: ...nyone other than authorized service personnel Unauthorized modifications or changes especially such that affect the safety of the staff and the environment are generally not allowed 3 8 Requirements for Operating Personnel The system may not be started up operated or maintained other than by duly trained expert personnel having received instructions on operational safety Such training will also in...

Page 34: ...chmark specifications and limit values applicable to the lifting and carrying of loads without the use of aids 3 10 Safety Notes for Operation 3 10 1 General Operating personnel are obliged to convince themselves of the proper technical condi tion of the system and its components including any safety devices before they can proceed to action for powering up This applies in particular after each mo...

Page 35: ...s Smoking or using open fire are forbidden inside of the operating room Operating personnel must be duly informed about the locations and the proper han dling of fire extinguishing equipment in the operating room 3 10 4 Electrics Working on electrical and electronic parts of the system and its components may only be carried out by a suitably qualified electrician according to latest binding electr...

Page 36: ...g wet piece of cloth to clean exterior surfaces Use only original accessory items and original spare parts or such accessories and spare parts that are recommended by the manufacturer refer to chapter Accessories Spare Parts Follow all advisory notes in chapter Maintenance Care 3 10 6 Handling of Dangerous Substances The system owner is solely responsible for compliance with all safety requirement...

Page 37: ...ble is not exhaustive Do not use solvents thinners aggressive detergents flammable liquids or etching leaches for cleaning Disregarding this advice may cause damage to system shielding or the opera tion control panel Component Material Pipette tips PP 2 5 μL ceramic tips Ceramic Capillary tips Glass PS polystyrene Piston seals1 PE HD Reagent cups PMMA or PTFE Tubes Silicone Wash tubs PEAK Reservoi...

Page 38: ...in a situation of danger make sure that the following requirements are met The operating staff must be familiar with the location of safety equipment accident and danger alarms as well as first aid and rescue equipment as well as their handling The operator is responsible for the respective training of the operating staff All equipment for first aid first aid kit eyewash bottles stretcher etc as w...

Page 39: ...echanisms are available CyBio Well vario with linear transporting unit CyBio Well vario with circular transporting unit Linear transporting unit The linear transporting unit is intended for motion of a 3 4 or 5 place carriage in one plane A carriage place is able to transport a microplate reservoir or a washing trough Depending on the product configuration a pipetting head can be replaced from a f...

Page 40: ...ctions are explained in the following chapters Fig 7 General view of CyBio Well vario head replacement from the front version Type plate Connection value label Fig 8 Type plate connection value label Information on the type label Manufacturer s information Product designations type designation trade name 1 3 4 2 1 Basic Unit 2 Transport rails 3 Tip magazine 4 Pipetting head ...

Page 41: ...ction as appropriate for a given microplate type 2 motors Placement of a microplate onto the carriage or rotary stage initial position Carriage motion in horizontal direction along the transport rails or rotary motion of rotary stage in horizontal direction Product version Head replacement from the front head change from the front The main switch for switching system power on and off is located on...

Page 42: ...n Keys for triggering lifter motion For down motion or up motion of the lifter If both keys are simultaneously pressed for a short while the lifter will move fully down to its zero position Keys for horizontal carriage or rotary stage motion The effect on pressing of these keys depends on the current lifter position Vertical position 0 mm Horizontal motion to carriage places or rotary stage places...

Page 43: ...nt removal or installation of a pipetting head the head has a handle attached to it and the magazine adapter provides a recessed handle Rails are located on the lateral sides of a pipetting head for guided installation or removal motion For exchange of pipetting head refer to page 78 The following types of pipetting head are optionally available See also price list Table 5 Choice of pipetting head...

Page 44: ...28 Rev B 2022 03 Technical Description CyBio Well vario Fig 10 Pipetting head with magazine adapter 1 2 3 4 1 Rail 2 Handle 3 Pipetting head 4 Magazine adapter ...

Page 45: ...pipetting head is detected by firmware when inserting into the basic unit Description Label volume Material Tips Drawing number Pos 4 Abb 111 Fixed TipTray 1 S 1 μl steel OL5021 24 5882 Coding magnet Fixed TipTray 2 G 8 μl glass3 OL5021 24 585 Re Fill TipTray 8 μl PP4 OL5021 12 511 1 For identifying the tray type when inserting into the pipetting head 2 Other variants see price list 3 Carrier mate...

Page 46: ...ront side front side or its backside back side depending on the particular product version see Fig 12 For convenient removal from or insertion into the system the capillary head has a special handle In addition a recessed handle in the magazine adapter can be used as further aid Rails mounted to the lateral sides of the capillary head provide for guided motion during removal or installation of the...

Page 47: ...apillary Head A magazine contains 96 or 384 capillaries A capillary consists of glass This means that a sample will only make contact with this inert material Depending on their assigned fixed volume the various capillary tips have color codes see Fig 14 Fig 14 Capillaries color code and volumes In cases of agglutination or breakage glass capillaries can be replaced individually refer to section 1...

Page 48: ...anel Fig 16 Connector panel for head replacement from the front version 1 Warning label 2 Information label 3 Connector panel 1 PSPL SV terminal for external device 2 COM RS 232 Sub D socket 9 pole 3 Terminal for operation control panel 4 CAN jack 5 Terminal for CyBio Well vario line power inlet 6 CyBio Well vario line fuse 7 Nameplate with product specs1 8 PSPL SV terminal for external device 1 S...

Page 49: ...on of the capillary wash station CAUTION Faulty pressure settings may damage the system Pressure settings made by customer service personnel must not be changed Make sure that the counterlocking nut below the setting wheel is properly tightened In the event of damage that can be shown to result from unauthorized changes in operating pres sure the manufacturer will refuse any liability TIP If the o...

Page 50: ...p for draining has a pre run and post run process and runs at a higher speed This prevents the wash tub from overflowing A sensor attached to the inlet tube directly above the supply bottle is used to monitor the proper supply of clean washing liquid In the event of an interruption in the washing liquid supply an error message is generated by the control software Suction tub The aspiration is achi...

Page 51: ...efer to page 33 Suction tub OL5021 14 600 4 3 3 Safety Notes The following safety symbols are affixed on the TWS module Fig 20 Warning symbols on the TWS module Danger areas Fig 21 Danger areas on the TWS module 1 Peristaltic pumps Safety symbol Meaning Comment Risk of crushing Warns of crushing of limbs by moving system parts 1 1 ...

Page 52: ...te bottle By means of vacuum the liquid is aspirated and directed into the waste bottle TWS module The TWS module is used for conveying and for aspirating liquids The module is equipped with two peristaltic pumps which can also be controlled individually The power supply to the system is provided by the corresponding pipettor or by a power supply module where the label specifying the permissible m...

Page 53: ...rial damage due to aggressive acids and lyes as well as organic solvents The tubes used in the peristaltic pumps come into direct contact with the liquid to be pumped Be sure to check the resistance of the tubes used against acids lyes and or ganic solvents Tubes made of silicone peroxide are supplied as standard TIP If you are unsure about the use of certain substances please contact Analytik Jen...

Page 54: ... 1 Peristaltic pump closed 2 Peristaltic pump open 3 Fixed tube By actuating the levers on the peristaltic pumps the pumps can be opened or closed mak ing it easy to insert or remove the tubes The tube lock holds the tubes in place This pre vents them from shifting during operation 1 2 3 4 5 6 7 1 B 1 Operating status indicator 2 B 2 BC Output for connection of a barcode reader 3 COM 1 Host PC 4 C...

Page 55: ...eft side move counterclockwise this means that the liquid is pumped from right to left the right tube end must be submerged in the storage vessel for washing liquid and the left tube end leads to the inlet of the washing tub The rollers of the outlet pump right side move clockwise this means that the liquid is pumped from the left to the right the left tube end is connected to the outlet of the wa...

Page 56: ...nner wall and can be removed for example with a subsequent washing cycle thus avoiding carryover However a small amount of liquid will always remain in the tips which corresponds to that in a manually pre moistened tip This behav ior is shown in figure Fig 28 Fig 28 Typical behavior of the residual liquid in normal pipette tips when resetting the pistons to the zero position 1 Tip in liquid after ...

Page 57: ... use of the dabbing station can prevent this carryover For the accurate transfer of small volumes reverse pipetting with additional stroke or addi tional volume is generally recommended in order to exclude falsification of the uptake vol umes due to capillary effects Residual ejection should always be performed on the dabbing station with activated vac uum in order to completely remove the residua...

Page 58: ...hicker and thus softer This better ensures that all tips touch down on the fleece and that the residual liquid thus comes into close contact with the fleece This is also supported by the fact that the surface of the dabbing station is mounted on springs The fleece itself is placed on a porous glass plate By means of a vacuum liquid is extracted from the fleece and transferred to a waste container ...

Page 59: ...ion e g after reverse pipetting or washing page 127 Example 2 Large volume of liquid in tips e g washing liquid or removal of liquid from wells e g for ELISA change of cell medium page 128 Example 3 Residual ejection of problematic liquids such as solutions containing surfactants or pro teins cell culture medium etc page 129 4 4 4 Important Instructions for Using the Dabbing Station Always use two...

Page 60: ...tes a specified volume of liquid from a trough Dispenses previously aspirated liquid into a microplate example with additional stroke Pipetting Aspirates slightly more than a predefined volume of liquid from trough with additional stroke volume Discharges predefined volume leaving residual volume in pipette tip differential pipetting Dispenses residual volume for example into a trough Dispensing A...

Page 61: ... of liquid must be transferred quickly and in parallel for example in Compound management Compound dry storage Assay development screening HTS ADME Tox Genomics proteomics Dispensing stock is aspirated by capillary force This means that 96 or 384 capillaries can be filled simultaneously using the effect of capillary force In principle a sample transfer consists of dipping the capillaries into the ...

Page 62: ...46 Rev B 2022 03 Technical Description CyBio Well vario ...

Page 63: ...or temperature variations with likely formation of con densed water mechanical shocks and vibration should be prevented Use separate packing for transportation of pipettor head and magazine adapter CAUTION Environmental influences mechanical shocks or formation of condensed water may destroy individual system components Adequate precautions should be taken to protect all components from environmen...

Page 64: ...mediately after arrival of product shipment or is not required for a longer period of time it should preferentially be stored in its original packing case Climatic requirements on facilities for system storage are as follows Temperature range 10 C to 50 C Allowable relative air humidity 85 at 30 C non condensing ...

Page 65: ...ite selection the following rules should be observed The operating room must have a stable horizontal dry and vibration free floor Do not install the system directly at doors or windows nor close to sources of electro magnetic interference Avoid direct sunlight and radiation from heaters onto the device Provide air condition ing for the room if necessary Provide easy access to all system parts at ...

Page 66: ...ic shock Never connect the mains plug of the device to a mains socket without a protective earth contact Make sure that the protection effect is not rendered ineffective by extension cables without a PE conductor or by the use of a voltage regulating transformer CAUTION Operation at a mains voltage level or frequency other than specified on the nameplate may result in destruction of the pipettor M...

Page 67: ...r measuring The photometer s own preci sion must be verified and documented according to manufacturer instructions prior to measurement Materials preparatory action 96 well or 384 well transparent flat bottom microplate prefilled with 0 1 N NaOH Tabelle 9 Prefill aspiration and test volumes auf Seite 52 Table 8 Prefill volumes Always use new tips for precision measurement Rinse new tips before mea...

Page 68: ...nufacturer instructions prior to measurement CyBio Well vario 96 well 250 μL CyBio Well vario 96 well 40 μl 25 μl CyBio Well vario 384 well 25 μl CyBio Well vario 96 384 well 60 μl Aspiration volume 20 μl 10 μl 10 μl 10 μl Standard test vol ume 10 μl 2 μl 2 μl 3 μl p nitrophenol dye solution 125 mg l in 0 1 N NaOH 600 mg l in 0 1 N NaOH 200 mg l in 0 1 N NaOH 125 mg l in 0 1 N NaOH 96 well micropl...

Page 69: ...te measured data as a preparatory stage of CV value determination Compare obtained CV result with CV values in chapter Technical Specifications on page 5 Procedure in case of individual variances 20 Result of leak testing on page 55 1 0 01 detergent e g Chaps Tween Triton x100 or similar TIP A certain inhomogeneity in the rate of evaporation over a microplate will influence the result of testing a...

Page 70: ...g a rinsing procedure as follows number of cycles 20 volume 25 μl CyBio Well vario 1536 number of cycles 10 to 20 volume 5 μl Dab off tips Accuracy testing is performed in manual Pipetting mode The appropriate volume is aspirated with an additional stroke of 0 5 μL Weigh the microplate in empty and lidded state Unlid the plate and place it onto the adapter Dispense the desired volume into the micr...

Page 71: ...oir to tips level the pipette tips should submerge by at least 2 mm below the surface of the test solution Moisten the pipette tips on the inside by performing five rinse cycles Aspirate 50 of end volume of dye solution Visually check the liquid level of all pipette tips over at time of two hours Document all tips with a non conforming liquid level Visual testing may be hard to perform with 384 we...

Page 72: ...roplate three times with water again after testing Result Typical values The p nitrophenol solution filling should have a concentration of approximately 120 μMol for measurement This is necessary to achieve an approximate absorption value of 0 6 This requires initial concentration values as follows Precision testing may also be combined with fluorescence measurement Rinse cycles 3x Rinsing liquid ...

Page 73: ...f the device head replacement from the back version to posi tion I siehe Abb 32 or Fig 33 Once power is available the CyBio Well vario is ready for action and can be oper ated in manual control or computer control mode Fig 32 Power on off switch on front panel Fig 33 Power on off switch on rear panel 7 2 Operating the CyBio Well vario from Operation Control Panel 7 2 1 Overview of Menu Items Pipet...

Page 74: ...ction For instructions regarding PC operating mode and PC interface you should refer to the CyBio Composer Plugin Pipet tor User Manual The CyBio Well vario can be operated from the operation control panel that is located beside the system Once system power is switched on various menus will be available in manual control mode each of which provides further options for selection and execution of su...

Page 75: ... for processing a single microplate Dosing This basic function provides for aspiration of a specified volume of liquid and dispensing of this volume with additional stroke 1 Select an aspiration volume Fig 38 Display screen dosing aspiration volume Konfiguration Hauptmenü Dispensieren Manuell Spitzenwechsel Tischweg dZ Wells Tischgeschw Klick Statistik Kopfwechsel Gerätenr OL xxx Kopfnr OL xxxx Zy...

Page 76: ...ing or as necessary to reach the required position To raise the lifter use to lower the lifter use Note With the lifter in raised position you can set the following positions via or 5 Confirm established position via key in order to trigger a volume dispensing cycle Fig 40 Display screen dosing dispensing The aspirated volume will be dispensed with a subsequent delay time as preset in the Configur...

Page 77: ...er be varied if necessary Fig 43 Display screen pipetting aspiration volume 3 Move a container with liquid into position under the tips trough microplate Trigger carriage or rotary stage motion using or as necessary to reach the required position To raise the lifter use to lower the lifter use Note With the lifter in raised position you can set the following positions via or 4 Select Start menu it...

Page 78: ...position via key in order to trigger a volume dispensing cycle The aspirated volume will be dispensed with a subsequent delay time as preset in the Configuration menu Fig 46 Display screen pipetting dispensing 8 Press keys and as required to bring a container into position under the tips for residual ejection 9 Confirm established position by pressing in order to trigger a residual ejection cycle ...

Page 79: ...n an according number of steps 1 Enter a value for number of subvolumes Fig 49 Display screen dispensing number of volumes 2 Define single dispense stroke volume Note Repeat this entry as many times as there are subvolumes to be processed Fig 50 Display screen dispensing dispensing volume 3 Select an aspiration volume Note The aspiration volume is preset to 2 μl above subsummed dispensing volume b...

Page 80: ...een dispensing aspiration 7 Move a microplate into position under the tips for dispensing Trigger carriage or rotary stage motion using or as necessary to reach the required position To raise the lifter use to lower the lifter use Note With the lifter in raised position you can set the following positions via or 8 Confirm established position via key in order to trigger a volume dispensing cycle S...

Page 81: ...e is dispensed once the cycle has started This is followed by a delay time as preset in Configuration menu Fig 55 Display screen dispensing residual ejection 12 In a break for dabbing you may use or as necessary to position the tips more conveniently for dabbing On termination of the dabbing break the pistons will return to zero position Fig 56 Display screen dispensing dabbing break Diluting Aspi...

Page 82: ...lowing positions via or 4 Select Start menu item Note On selection of Exit you can exit the Manual menu without actually triggering the process Fig 59 Display screen diluting start 5 Use key for confirmation This is necessary to trigger a dilution process 6 To trigger aspiration of subvolumes press key Note Repeat this process as many times as there are subvolumes Fig 60 Display screen diluting as...

Page 83: ...or dabbing On termination of the dabbing break the pistons will return to zero position Fig 62 Display screen diluting dabbing break Tip replacement 1 Select tip change basic function in Manual menu The magazine plate with pipette tips is automatically released Fig 63 Display tip change tip release 2 Remove tip magazine from the pipettor 96X pipetting head Motion to positions A1 to B2 for 384 well...

Page 84: ... with tips prompt Fig 66 Display tip change magazine insertion 4 Confirm clamped state by pressing key Note Once the magazine plate is fixed the screen will display the menu in which a tip change was selected 1 Magazine plate 2 Pipette tips CAUTION There is danger of piston or system damage Remember that only tip filled magazines may be inserted and clamped Insertion of a magazine is monitored by ...

Page 85: ...ove a rinsing vessel into position under the tips for rinsing Trigger carriage or rotary stage motion using or as necessary to reach the required position To raise the lifter use to lower the lifter use 3 Confirm established position by pressing key Fig 69 Display screen rinsing volume 4 Trigger rinsing by clicking key Fig 70 Display screen rinsing start The screen will alternately display Aspire ...

Page 86: ...d means for processing a single microplate Dispensing A total volume that depends on the type of capillary is aspirated by capillary force Com pressed air is then applied to dispense this volume into the microplate Select item Manual in the main menu This will display the following box Fig 74 Display dispense tip change Select the dispense menu item This will display the following box Fig 75 Displ...

Page 87: ...igger position by pressing lower the lifter by pressing so it will move out of the microplate wells Note With the lifter in this position you can use or as necessary to set the following XY positions Fig 76 Display dispense with position selection 4 Select Start menu item Note On selection of Exit you can exit the Manual menu without actually triggering the process Fig 77 Display dispensing start ...

Page 88: ... Display tip change tip release 2 Remove tip magazine from the pipettor Fig 79 Display tip change magazine removal Fig 80 Capillary magazine change version head replacement from the front CAUTION There is danger of finger pinching and damage to capillary tips The magazine plate is released and clamped with electro motoric power For a tip change the magazine plates must be inserted until hard stop ...

Page 89: ...ed the screen will display the menu in which a tip change was selected Fig 82 Display tip change tightening of tips CAUTION Prevent damage to the system Only capillary filled magazines may be inserted and clamped Insertion of a magazine is mon itored by a microswitch If a magazine is placed in a non conforming manner an Insert mag azine with tips prompt will appear Should this microswitch fail the...

Page 90: ...ill only be valid for manual mode Stage Motion Length For defining a maximum available length of stage motion lifter height This setting pre vents mechanical collision of a vessel with tips It also facilitates different applications for example the picking up of liquid over a cell lawn or the pipetting onto the bottom of a microplate from a preselected distance The height position can be adjusted ...

Page 91: ...int nn corre spond to those of a 96 well microplate The path however represents the distance to a 384 well or 1536 well in the correspond ing coordinate direction refer to details on underside of lifter On selection of a coordinate the lifter moves to the assigned position and can thus be eas ily inspected for correct adjustment A microplate of transparent material is recommended for lifter adjust...

Page 92: ...tance The height position can be adjusted in steps of 0 1 mm dZ For updating the vertical setting value for the lifter following necessary replacement of the lifter setting value inscribed on lifter bottom on shipment This will automatically correct vertical lifter adjustment so previously edited programs need not be changed in terms of height coordinates Wells This option can be used to select a ...

Page 93: ... values The first entries in a line indicate the direction The coordinates for zero point nn corre spond to those of a 96 well microplate The path however represents the distance to a 384 well or 1536 well in the correspond ing coordinate direction refer to details on underside of lifter On selection of a coordinate the lifter moves to the assigned position and can thus be eas ily inspected for co...

Page 94: ... released Fig 89 Display main menu 2 Remove tip magazine from pipetting head and store magazine in tip tray package Fig 90 Display head change remove magazine CAUTION Risk of crushing Keep clear of the pipettor head s working and motion range unless operation has stopped Otherwise there is high risk of physical injury Notably make sure that all protective covers and shielding panels are mounted an...

Page 95: ... the pipetting head until mechanical stop position Fig 92 Display head change insert magazine adapter Fig 93 Insert magazine adapter head replacement from the front version 4 Confirm magazine adapter inserted state by pressing key The adapter is automatically clamped Fig 94 Display head change clamp magazine adapter 5 Wait until prompt for removal of head appears then confirm by pressing key ...

Page 96: ... the system not at the head see Fig 121 Hold pipetting head at its handle with one hand Slightly raise the pipetting head Carefully pull the head off supporting it with the other hand Use recessed handle at magazine adapter bottom for this purpose Place pipetting head onto a stable support base CAUTION There is danger of crushing or pinching as the cover is opened or closed To open or close the co...

Page 97: ...recessed handle at magazine adapter bottom for this purpose Hold head rails at the height as indicated by red markers then push the pipetting head in until mechanical stop position Only for capillary head Connect compressed air supply tube to terminal connector at the system Check for correct setting of operating pressure Fig 98 Insertion of pipetting head head replacement from the front version 1...

Page 98: ...e into pipetting head until mechanical stop position Fig 101 Display head change insert magazine CAUTION Risk of crushing To open or close the cover hold its handle with one hand Keep the other hand clear of the system space CAUTION There is danger of piston or system damage The pipetting head may only be loaded with magazines filled with tips or capillaries Insertion of a magazine is monitored by...

Page 99: ...em is ready again for operation Head replacement from the back To perform head replacement for from the back version proceed as follows 1 Press key or in the main menu as necessary to select the Head change menu then confirm your selection with Fig 103 Display main menu 2 Remove tip magazine from pipetting head and place magazine into its special storage box Fig 104 Display head change remove maga...

Page 100: ...magazine adapter down into the pipetting head until mechanical stop position Fig 106 Display head change insert magazine adapter Fig 107 Insertion of magazine adapter head replacement from the back version 4 Confirm magazine adapter inserted state by pressing key The adapter is automatically clamped Fig 108 Display head change clamp magazine adapter ...

Page 101: ...ed air supply tube do not detach connector at the head end see Fig 121 Hold pipetting head at its handle with one hand Slightly raise the pipetting head Carefully pull the head off supporting it with the other hand Note Use recessed handle at magazine adapter bottom for this purpose Place pipetting head onto a stable support base CAUTION There is danger of crushing or pinching as the cover is open...

Page 102: ...ers then push the pipetting head in until mechanical stop position Only for capillary head Connect compressed air supply tube for head with inlet terminal of com pressed air control unit Check for correct setting of operating pressure at compressed air control unit Fig 112 Insertion of pipetting head head replacement from the back version 9 Tilt cover down until it closes then release cover The co...

Page 103: ...p magazine into pipetting head until mechanical stop position Fig 115 Display head change insert magazine CAUTION There is danger of piston or system damage Insert only tip filled magazines into the pipetting head Insertion of a magazine is monitored by a microswitch If a magazine is found to be wrongly inserted an Insert tip magazine prompt will appear A microswitch failure may result in damage t...

Page 104: ...ure for powering on CAUTION There is danger of damage to the system due to incorrect parameter settings following a reset of operating parameters Operating parameters are preferably set by the customer service of the manufacturer Before resetting an operating parameter and setting a new value by yourself make sure you have consulted with the appropriate customer service personnel at first Button S...

Page 105: ...system parameters must be set manually again Drive parameters Are valid for powering of the pump motor piston drive the carriage rotary stage motor and the lifter motor Starting rpmm End rpm Number of steps to reach end rpm Device parameters Language Waiting Time Maximum lifter height Lifter height compensation dZ Plate type selection available for 96 well head operation only XY stage shift settin...

Page 106: ...an proceed as follows Washing procedure Solution for alkaline washing 10 RBS35 solution Carl Roth GmbH item no 9238 a Keep submerged in 10 RBS35 solution for 30 minutes submer sion depth of capillary tips about 4 mm b Perform 10 washing runs with distilled water aspiration time 8 seconds dispensing 1 second at 25 psi Solution for acidic washing Universal decalcificator e g sidol 1 1 diluent a Keep...

Page 107: ...tion starts in zero position 1 and continues beyond the position that corre sponds to nominal pipetting volume The pistons are then lowered back into this position 2 The amount of freeplay 3 is excluded by this procedure At the time of direction reversal an additional volume D1 is dispensed Due to piston reversal this volume is smaller than the aspirated additional stroke volume A2 The tip retains...

Page 108: ...n a single step in order to achieve precise results This cycle must provide for a motion length that is greater for dispensing 4 than that for aspiration 2 Since the reversal point of piston motion lies within the motion range that is covered by the pistons for dispensing no vol ume is dispensed on excession of the upper freeplay 3 If dispensing motion covers a motion length that is greater than a...

Page 109: ...thout additional stroke Dispensing with additional stroke Motion to zero position 7 4 4 Waiting Time Piston motion always creates a state of underpressure or overpressure during an aspiration or a dispensing cycle In order to maintain the specified system precision pressure equal ization in the tips must be allowed to finish This is supported by a specific Interruption software command A waiting t...

Page 110: ...id being handled For this reason adequate pause times should be included with all operating sequences as necessary TIP When working with dry tips pressure equalization of a different kind will occur after filling It takes a longer to finish because an air vapor pressure will build up in the air cushion inside the tip above liquid level resulting in liquid emerging from the tips This can be prevent...

Page 111: ...ce again Tip magazine of older make back corners are not rounded Replace tip magazine or have magazine reworked by dealer service personnel Tip magazine edges or corners deformed resulting from drop impact Replace tip magazine or have deformation removed 7 System error Notify customer service 8 Failure to find internal zero position of pump system You may be able to remove this error by performing...

Page 112: ...arame ters 22 Error on carriage motion or rotary stage motion Remove obstacle or clean car riage and transport rails or rotary stage 24 Command cannot be executed at this moment Wait until previous command has finished 26 Z drive motion error Switch power supply off and on again 27 Failure to detect pipetting head Unseat pipetting head once again then restore it to working position Switch system p...

Page 113: ...system state A command was triggered but cannot be successfully completed in the present system state for example command attempted switching the XY position controller for 384 well microplates to a new posi tion while vertical drive motion was out of switching range limits Change parameter settings of fault triggering com mand to permissible values Wrong parameters Software transferred wrong para...

Page 114: ...ernal zero position of piston motion drive System was unable to find the zero position for piston motion Perform a change of tips Repair work is necessary if this error persists No tip magazine available System failed to detect a tip magazine Insert a tip magazine if no tip magazine available Remove and reinsert tip magazine if a magazine had been installed No or faulty communica tion with X Y sta...

Page 115: ...its upper end posi tion Remove this error by per forming an exchange of tips If the error persists repair work is required Notify customer service Piston drive failed to reach lower end position Piston motion has not reached its lower end posi tion Remove this error by per forming an exchange of tips If the error persists repair work is required Notify customer service Sum of all volumes in a prog...

Page 116: ...t than the currently installed software version Use latest software version in this case If this can be discarded as potential error cause the particular file is likely to have suffered dam age Create a new program file Volume too small The sum of all volumes aspirated and dispensed is too small Valid values are within the range of 2 μl to 250 μl If all signed cycle values are added up with each o...

Page 117: ...ressure at output Fixed opening clogged Replace component or fixed opening customer service Compressed air leakage at outlet opening Dust in sealing part of main valve Remove dust from valve seat by replacement of seat com ponent or by cleaning of valve guide customer service Membrane has a defect Replace membrane customer service ...

Page 118: ...102 Rev B 2022 03 Troubleshooting CyBio Well vario ...

Page 119: ...e no unlimited lifetime WARNING Note that contact with voltage carrying system parts may lead to physical injury or even death Switch system power off and detach power cable from the line socket before you pro ceed to any kind of maintenance or care Make sure that the system is protected from accidental restoration of power Operating personnel are prohibited from performing work for maintenance re...

Page 120: ...llary head refer to section 19 2 9 x Clean all liquid holding vessels and the outside of tubes with disinfectant x Clean tubes with disinfectant or autoclave tubes x Inspect sealing plate pipetting head for clean condition x Inspect carriage or rotary stage for visible dam age and clean as necessary Remove tip magazine Switch power off Remove microplates or reservoirs x Only linear transporting un...

Page 121: ...uid in affected tips will reach an excessively high fill level There is danger of pistons coming into contact with liq uid and suffering contamination in such cases This may happen if liquid is drawn from ves sels with insufficient filling level or if tips are not introduced deep enough Make sure that pistons cannot come into contact with liquid during motion into zero posi tion Repeated faulty op...

Page 122: ...individual capillaries as required using the tool supplied To do this use Allen key to unscrew a defective capillary Then install and fix a new capillary 5 Install the capillary magazine back into the capillary head and press Enter key at the control panel The capillary magazine will be clamped again Its capillaries have been replaced CAUTION Glass capillaries are fragile There is danger of physic...

Page 123: ... power plug from the socket 2 Remove tip magazine from pipetting head for safety reasons refer to page 104 3 Remove all microplates reservoirs or washing troughs 4 Clean the rotary stage using a mild detergent or disinfectant 5 Carefully push tip magazine back into the holder at the pipetting head Make sure that tip magazine is correctly placed on completion of work 6 Reconnect power plug to mains...

Page 124: ...ck the view glass at the compressed air control unit for condensate level If liquid can be perceived in its lower part hold a piece of cloth below the drain screw and remove condensate by releasing the drain screw see Fig 120 Fig 120 Compressed air control unit condensate drain outlet CAUTION Danger Splashing When draining condensate hold a piece of cloth underneath the drain screw ...

Page 125: ...pply off and drain the system space until pressure relieved state is achieved 7 Switch system power supply off using main switch or socket bar for this purpose 8 Disconnect power cord from socket bar or line power socket 9 Disconnect compressed air supply tube from inlet connector at compressed air control unit 10 Remove all microplates 11 Clean and disinfect the pipettor and its components as des...

Page 126: ...110 Rev B 2022 03 Decommissioning CyBio Well vario ...

Page 127: ...tting head 384 fs OL3381 24 630 CyBio Well vario60 μl pipetting head 384 fs OL3381 24 680 CyBio Well vario8 μl pipetting head 1536 fs OL5021 24 130 Pipetting heads exchangeable from the back Ordering code number CyBio Well vario2 5 μl pipetting head 96 bs OL3381 24 235 CyBio Well vario25 μl pipetting head 96 bs OL3381 24 735 CyBio Well vario40 μl pipetting head 96 bs OL3381 24 755 CyBio Well vario...

Page 128: ...ed information about available Pipette tips and magazines you should consult our latest catalog or visit us on the Internet 96 channel and 384 channel capillary magazines including 10 reserve capillaries 25 nl capillary magazine 50 nl capillary magazine 100 nl capillary magazine 250 nl capillary magazine 500 nl capillary magazine 750 nl capillary magazine 1000 nl capillary magazine Spare capillari...

Page 129: ...code number Label Fixed TipTray 1 1 μl steel OL5021 25 5881 S2 Fixed TipTray 2 8 μl Glass OL5021 25 5853 G Re Fill TipTray 8 μl PP4 OL5021 25 5115 1 In case 2 With coding magnet 3 In case 4 Polypropylene 5 Tray Description1 Comment Ordering code number Dabbing fleece Exchangeable insert for suction tub OL5021 14 600 OL5021 25 615 1 Status of the compilation 08 2015 ...

Page 130: ...114 Rev B 2022 03 Accessories Spare Parts CyBio Well vario ...

Page 131: ...e safety and environmental provisions of law ENVIRONMENTAL PROTECTION Biological samples must be treated in accordance with locally binding regulations for the handling of infectious material Hazardous substances and related containers may not be disposed as domestic waste nor be allowed to penetrate into a sewage system or the soil The appropriate rules and practices must be closely followed for ...

Page 132: ...116 Rev B 2022 03 Waste Disposal CyBio Well vario ...

Page 133: ...oser software controls CyBio Well vario system operation For specific stacker information you should consult the Stacker User Manual A 1 1Overview Because of its modular setup the system is available in a variety of configurations To pro vide an example of CyBio Well vario system configuration a CyBio Well vario with linear transporting unit 5 positions and two stackers with peristaltic pump is sh...

Page 134: ...a desk top device Make sure the laboratory bench has ade quate load bearing capacity compare with weight specifications before you install the system Dimensions Max width 1640 mm Height with stackers standard version 850 mm Depth 445 mm Weight CyBio Well vario System with 2 stackers ca 80 kg w o accessories Utility class Desk top device for use in enclosed and clean spaces Protection class 1 Inter...

Page 135: ...ed to follow all safety notes refer to chapter 3 in the CyBio Well vario User Manual and the Stacker User Manual 1 1 2 2 3 3 4 5 1 Stop and Down pushbutton for stacker 2 Warning text at peristaltic pump 3 Warning notes at stacker lifter 4 Warning label at pipetting head 5 Warning note at barcode reader and warning text 1 2 3 1 3 1 Lifter of stackers 2 Lifter of pipettor 3 Peristaltic pump ...

Page 136: ...g module Fig 125 Setup versions on base frame 1 Base frame 2 Mounting plate for pump module accessory item and extension module accessory item 3 CyBio Well vario 4 Stacker mounting plate in remote location 5 Stacker mounting plate in close location Name Quantity CyBio Well vario Number of stack ers Stacker CyBio Well vario 1 1 CyBio Well vario stacker 1 1 Stacker CyBio Well vario stacker 1 2 EXT s...

Page 137: ...nufacturer or that of specifically authorized contractors P S P L S W B C Stacker links M aster B ar code reader P C 3 fach S chalter F üll stands sensor S chutzleiter im Gestänge S chutzleiter im Gestänge F üll stands sensor F üll stands sensor F üll stands sensor B ar code reader 3 fach S chalter 3 fach S chalter B ar code reader CyBi Well vario P S P L P S P L COM COM1 COM1 COM2 COM3 COM2 COM3 ...

Page 138: ... tube end must be submerged in the reagent vessel while the right tube end connects to the reservoir A 1 5 3Insertion of Tube into Peristaltic Pump 1 Switch peristaltic pump lever to the left Pump is open 2 Insert tube in same direction as shown in chapter A 1 5 2 3 Return lever of peristaltic pump into rightmost position 4 Push right and left tube locks into locked position Pump is closed and rea...

Page 139: ...ion refer to Plugin Pipettor Software ManualCyBio Composer A 1 6 2PC Mode PC interface Data transfers between the CyBio Well vario stackers additional modules and the control computer are accomplished via a serial RS 232C interface Characters are of uneven parity each consisting of 8 data bits and 2 stop bits This data format cannot be changed The baud rate is fixed at 19200 Baud It cannot be chan...

Page 140: ...ional table that is provided below in order to check for all potential error sources Should difficulties persist or faults develop after this check other than described in the rel evant module error tables and the table below you are requested to notify the customer service of the manufacturer or your authorized service agent TIP All advisory notes refer to chapter 9 in the CyBio Well vario User M...

Page 141: ...oted on page 9 of chapter Technical Specifications System reports tip maga zine not inserted error Tip magazine of older make back corners are not rounded Replace tip magazine Tip magazine edges or cor ners deformed resulting from drop impact Replace tip magazine Carriage fails to reach posi tions motor drive makes rumbling noise Tight carriage motion Raise carriage from sliding rails and clean ca...

Page 142: ...126 Rev B 2022 03 CyBio Well vario ...

Page 143: ...le 1 1 OL5012 14 600 for description and function see section from page 40 Move to dab location Switch on vacuum Move to dab height Residual ejection with blowout Waiting time Remove tips from the fleece Waiting time Contact dab again Waiting time Switch off vacuum Lower lifter Move pistons to zero position tips have contact with the fleece ...

Page 144: ...ng station very close to Residual ejection with blowout Waiting time Remove tips from the fleece Waiting time Contact dab again Waiting time Switch off vacuum Lower lifter Move pistons to zero position Contact with fleece fleece no contact Waiting time Ejection of the nominal volume i e the volume taken up ...

Page 145: ...ional blowout volume at the end of the cycle the 1 μl additional volume taken up as air gap at the beginning of the procedure is not included in the script excerpt shown below Fig 134 Example 3 Move to dab location Switch on vacuum Move the dabbing station very close to Residual ejection 1μl Waiting time Remove tips from the fleece Waiting time Contact dab again Waiting time Switch off vacuum Lowe...

Page 146: ...130 Rev B 2022 03 CyBio Well vario ...

Page 147: ... air supply unit 33 Configuration 51 Conforming use 2 Connector panel 32 Conventions 1 D Dabbing fleece 42 Data transfer 123 Decommissioning 109 Delivery of liquid 122 Detergent 53 Device versions 120 Diluting 44 65 Dimensions 118 Direction of rotation 122 Dispensing 44 45 63 70 Dispensing with additional stroke 92 Dosing 44 59 dZ 74 76 E Enter key 26 Error 95 101 Error code 95 Error message 97 Ex...

Page 148: ...9 R Remote location 120 Reservoir filling station 122 Rotary stage 23 S Safety notes 119 Scope of delivery 4 Sealing plate 107 Serial number II Setup versions 120 Site requirements 49 Solenoid valve 33 Source plate 45 Spare parts 111 Spatial requirements 49 Special functions 88 Specific system features 91 Stacker 117 Stage motion length 74 76 Stage speed 75 77 Start up 51 Startup 121 Stop and Down...

Page 149: ...onnections 38 Danger areas 35 Operation 36 V Vacuum extraction 33 Ventilation devices 18 Volume cycle 93 Volumes 27 30 W Waiting Time 74 Waiting time 93 Warranty liability 3 Wash tub Technical specifications 39 Washing liquid 34 Waste disposal 115 Weight 118 Wells 74 76 X XY 75 77 ...

Page 150: ...134 Rev B 2022 03 CyBio Well vario ...

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