E
lectron
M
icroscopy
S
ciences
P.O. Box 550
1560 Industry Road Hatfield, PA 19440
TOLL FREE:
1-800-523-5874
TEL:
215-412-8400
WEB:
FAX:
215-412-8450
EMAIL:
An introduction
The NIGHTSEA
DFP™ Series Dual Fluorescent Protein Excitation Flashlight is a dual color light source
that allows the viewing of transgenic organisms expressing GFP, fluorescein, Lucifer yellow, YFP, Venus,
and more. The light uses LEDs and interference filters in order to initiate intense excitation. With the filter
glasses, the light is effective for screening juveniles to determine if they are expressing the fluorescent
protein or not, to view the progress of the experiments, and for photography.
Operation
The three-position locking switch can be found in the front of the handle. The three positions are:
(Left)
ROYAL BLUE LIGHT
(Center)
OFF
(Right)
CYAN LIGHT
1.
Unlock the switch by pulling the lock down from the end of the switch.
2.
Rotate the switch to the left or right, depending on the light color you desire.
3.
Turn off the light by rotting the switch back to the center position.
4.
To prevent accidental actuation, push the lock back into the switch.
The DFP™ is supplied with:
Two (2) pairs of filter glasses
– One (1) yellow and one (1) orange
Use the yellow glasses with the royal blue excitation and the orange glasses with the cyan excitation. The
filter glasses are matched to the spectra of the light sources to reject the reflected excitation light and
transmit the fluorescence with high contrast.