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Suck in the mobile phase with the plastic syringe as long as bubbles come out from the pump. It is
possible to switch the valves during washing, by pressing keys with arrows, this allows the liquid to
penetrate to all necessary channels Press key PUMP again to stop the pump washing. Tighten the
purge/degass valve and take out plastic syringe.
For repeated degassing/purging of the unit, it is
possible to use the waste tubing, mounted to
purge/degass valve.
If waste tubing is put on purge/degass valve, it is
not possible to close the unit door!
In chapter
it is possible to set parameters for
washing.
Now the flow can be set by pressing key FLOW.
Use arrows left and right to move with cursor and
arrows up and down to change value. Press
ENTER key to confirm the value, press PUMP to
start the pump. Press PUMP again to stop the
pump.
For fast setting of one of the valves to 100 %, it is
possible to use arrow with the letter of the
appropriage valve. This switching is functional in
gradient mode during modes IDD, PRG and PMP
(see
).
Note:
We recommend to run and stabilize the devoce before starting analyzes or
after a longer shutdown of the device at 1 ml/min, pressure 15-20 MPa for
approximately 30 min.
Note:
Run the gradient pump with a 25 % concentration setting for each valve
approximately 10 min.
4.11. Description of Symbols on the Screen
Value/Symbol
Meaning
10%20%30%40%
0 ml
Actual gradient concentration for A, B, C, D.
In isocratic mode is displayed remaining mobile phase volume.
12.0-15.0
Value of low
–
high pressure limit in actually set pressure units.
1.00ml/min
Actual flowrate value.
13.0MPa
Actual pressure value.
