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Drucker Diagnostics QBC 425740, Service Manual

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Drucker Diagnostics QBC 425740 Service Manual Download Page 19

5.4 SPECIMENS FOR QBC MALARIA TEST

Collect capillary blood from the finger or foot, directly into the QBC Malaria Test tube; or draw venous blood and 
collect into the tube. Fill the tube with 55 to 65 µL of blood. QBC Malaria Test tubes are pre-coated with sodium 
heparin and dipotassium EDTA anticoagulants.

Stability: Centrifuge the blood-filled QBC Malaria Test tubes as soon as possible after filling. See 6.4.4 for 
extended stability of the QBC Malaria Test prior to microscopic examination.

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Summary of Contents for QBC 425740

Page 1: ... QBC Capillary Centrifuge Operator s Service Manual ...

Page 2: ...erator s Service Manual QBC QBC Diagnostics ParaLens and ParaLens Advance are trademarks of Drucker Diagnostics Copyright 2016 by Drucker Diagnostics and Vacutainer is a trademark of Becton Dickinson Company 4740 020 004 Rev J IVD ...

Page 3: ...740 5 2 4 2 QBC Power Pack part no 421763 5 Section 3 INSTALLATION AND SET UP 6 3 1 PACKAGING AND PARTS 6 3 2 INSTALLATION AND SERVICE 6 3 3 CONNECTING POWER UNIT 7 3 4 PLACEMENT AND OPERATING ENVIRONMENT 7 3 5 ROTOR ASSEMBLY 8 Section 4 CENTRIFUGE OPERATION 9 4 1 GENERAL PROCEDURES 9 4 2 BETWEEN SPIN TIME DELAY 9 4 2 1 QBC Centrifugal Hematology Tubes 9 4 2 2 QBC Malaria Test 10 4 3 TUBE LOADING ...

Page 4: ...2 Loading and Balancing Rotor 16 6 4 3 Centrifugation Procedures 16 6 4 4 Testing Time 17 Section 7 LIMITATIONS OF PROCEDURES 18 7 1 QBC CENTRIFUGAL HEMATOLOGY TESTS 18 7 2 QBC MALARIA TEST 18 Section 8 CALIBRATION CHECKS 19 8 1 CHECKING ROTOR SPEED 19 8 2 CHECKING TIMER ACCURACY 19 Section 9 MAINTENANCE AND SERVICE 20 9 1 GENERAL CLEANING 20 9 2 ROTOR MAINTENANCE 20 9 2 1 Periodic Inspection 20 9...

Page 5: ...iv ...

Page 6: ...ically positioning the float within the buffy coat layer between the packed red cells and the plasma QBC tubes for hematology are produced in a standard design which contain an unassembled float and tube closure Under centrifugation blood cells in the buffy coat layer separate according to their densities forming visibly discrete bands as shown in Figure 1 2 Platelets constitute the topmost band l...

Page 7: ...he centrifuged blood under a fluorescence microscope or a light microscope with the QBC ParaLens AdvanceTM LED fluorescence microscope attachment readily permits the detection of malaria in the infected cells and plasma Direct malaria detection by the QBC Test method is therefore more rapid than conventional blood film microscopy 1 3 PRINCIPLES OF THE PROCEDURES 1 3 1 QBC Centrifugal Hematology Te...

Page 8: ...ytes monocytes where the parasites are most abundant For technical assistance with the QBC Malaria Test contact 1 866 265 1486 toll free 1 814 692 7661 Drucker Diagnostics 168 Bradford Drive Port Matilda PA 16870 U S A 1 4 WARNINGS AND PRECAUTIONS Always observe good laboratory procedures when handling patient specimens Consult Section 4 6 of this manual for detailed safety precautions IMPORTANT T...

Page 9: ...uction feet for added stability A protective metal cover which threads onto the motor shaft keeps the spinning tubes snugly in place on the rotor The hinged top lid with safety latch provides a housing or shield around the rotor during operation Electrical power to the centrifuge is controlled by a momentary ON OFF power button located on the front deck Before the motor will start the top lid must...

Page 10: ...matology 20 to 32 ºC 68 to 90 ºF For Parasite Detection 16 to 37 ºC 60 to 99 ºF Functional Range Centrifuge Operation 2 to 40 ºC 36 to 104 ºF Operating Relative Humidity 0 to 80 for temperatures between 2 and 31 ºC derating linearly to 50 between 31 and 40 ºC Non Operating Storage Temperature Limits 26 to 66 ºC 15 to 150 ºF Weight with rotor and cover installed 2 8 kg 6 2 lbs Dimensions with lid c...

Page 11: ... alternate line cord is provided with the Power Pack Select the appropriate line cord and discard the unused cord 3 2 INSTALLATION AND SERVICE For first time users of the QBC System a representative of Drucker Diagnostics or your local QBC System distributor will normally install the required equipment the installer will also train operating personnel in proper use of the equipment If necessary co...

Page 12: ...its suction feet Mark an envelope 300 mm 12 inches wider than the body of the centrifuge around the area in which the centrifuge is to be used Keep all personnel and hazardous materials outside this envelope when the centrifuge is operating CAUTION To avoid electrical hazards and equipment damage connect the power cord only to grounded receptacles delivering the voltage and frequency specified on ...

Page 13: ...rside of the rotor hub are aligned with the drive pin in the motor shaft Figure 3 4 b Press the rotor down until the drive pin is seated in the hub slots and 3 5 threads of the shaft protrude above the hub about 6 mm c Thread the head nut onto the shaft finger tight by turning it clockwise Tighten with a light torque using the head nut wrench supplied with the centrifuge Figure 3 5 DO NOT OVERTIGH...

Page 14: ...buildup in the rotor compartment which may adversely affect cell layer formation Accordingly between successive spins be sure to wait the appropriate time specified below IMPORTANT During each between spin rest be sure the rotor cover is removed and the rotor empty 4 2 1 QBC Hematology Tubes If room temperature is 24 ºC or less 75 ºF wait 1 min between spins If room temperature is 25 28ºC 75 82 ºF...

Page 15: ...atically released when the Power switch of the centrifuge is off provided the Power Pack is on and connected to the centrifuge When a spin cycle is completed the latch releases after a momentary delay and the lid pops up Figure 4 2 Place tubes on the rotor in a balanced array with the tube closures against the outer rim of the rotor To maintain balance insert the tubes consecutively starting at sl...

Page 16: ...e Power Pack back in This will disengage the electric latch interlock and cause the lid to open Note For procedures on opening the lid in the event that main power to the centrifuge is lost through a fuse or main electrical failure see Section 9 3 4 5 SPEED POWER LED MONITORS The yellow POWER and green SPEED LED lights are used for monitoring the status of the centrifuge When both lights are on st...

Page 17: ...handling all items contaminated with blood or other body fluids Exercise care when using QBC tubes The tubes are made of glass and may break possibly causing injury and exposure If a tube breaks carefully remove broken glass with a hemostat or other device using puncture resistant utility gloves and dispose of properly Disinfect rotor after a tube break See Section 9 2 2 For continued safety perio...

Page 18: ...QBC hematology test analysis can be collected in Vacutainer brand tubes containing EDTA anticoagulant QBC tubes for venous blood are filled by means of a semi automatic pipetter supplied with the QBC System QBC capillary blood tubes are pre coated with anticoagulants and are filled with blood directly from a finger puncture After filling with blood the tubes are sealed with a rubber stopper or pla...

Page 19: ...nd collect into the tube Fill the tube with 55 to 65 µL of blood QBC Malaria Test tubes are pre coated with sodium heparin and dipotassium EDTA anticoagulants Stability Centrifuge the blood filled QBC Malaria Test tubes as soon as possible after filling See 6 4 4 for extended stability of the QBC Malaria Test prior to microscopic examination 14 ...

Page 20: ...s Box of 100 420001 Work Station 424226 Sterile wipes 6 2 2 QBC Malaria Test The following disposables and test accessories are required for the QBC Malaria Test Accessory Part No QBC Malaria Test Box of 100 253037 QBC Malaria Test Box of 1000 253005 QBC ParaLens Advance with 60x Objective 424331 QBC Microscope 424294 Work Station 424226 Sterile wipes 6 3 TEMPERATURE REQUIREMENTS For QBC Hematolog...

Page 21: ...t to re use the balance tube for patient samples 6 4 3 Centrifugation Procedures a Secure the cover to the rotor then close and firmly latch the lid b Press and release the ON button to start the spin cycle c After the five min spin cycle is complete and the lid pops up open the lid and REMOVE THE TUBES IMPORTANT Failure to remove tubes promptly after centrifugation may affect cell layer boundarie...

Page 22: ...ubes in the QBC Analyzer 4 hours after centrifugation Store the tubes vertically stopper down in the tube rack away from heat and intense light Consult the applicable QBC System Operator s Manual for test instructions QBC Malaria Test After centrifugation QBC Malaria Test tubes can be held for as long as 3 days unrefrigerated at temperatures up to 37 ºC 99 ºF before microscopic examination Store t...

Page 23: ...ethod for diagnosing positive or negative patients While numerous reports have demonstrated that the QBC Malaria Test is significantly more sensitive for detecting malaria than stained blood films the QBC Test may not detect very low levels of parasitemia Experienced microscopists can discriminate between various species of malarial parasites using the QBC Malaria Test Examination of a thin blood ...

Page 24: ... contact strobe tachometer to assure that speed is sufficient to produce visibly distinct and differentiated cell layers in the QBC tube A window in the top lid and a reference line on the knob of the rotor cover are provided for speed checks with a non contact tachometer 8 2 CHECKING TIMER ACCURACY The electronic timer of the Centrifuge is designed to be accurate to 300 15 s Check the timer for a...

Page 25: ...sponsibility of the user to observe and follow appropriate decontamination procedures Handle all patient specimens as potential biohazards capable of transmitting infection When cleaning the instrument always wear appropriate personal protective equipment including laboratory gloves In addition to wearing gloves the use of disposable lab coats or gowns and protective glasses or goggles is recommen...

Page 26: ...roy any viral and bacterial contaminants BE SURE TO REMOVE THE ROTOR PROMPTLY AFTER 10 MIN OF SOAKING IN THE BLEACH SOLUTION SINCE EXTENDED EXPOSURE TO THE BLEACH CAN CORRODE THE ROTOR b Rinsing and Drying After soaking in the dilute bleach solution specified above completely immerse the rotor in clean water Rinse again under running water to remove all traces of bleach Thoroughly dry the top and ...

Page 27: ...ned as follows a Disconnect the Power Pack plug from the centrifuge b Turn the centrifuge upside down Using a Flat head screwdriver locate in the center the emergency lid open slot c Push gently on the end of the solenoid plunger until the latch is released Figure 9 1 9 2 d Turn the centrifuge upright Reconnect the plug of the Power Pack to the centrifuge e When the power problem is corrected chec...

Page 28: ...inning An electrical interlock prevents the lid from opening until rotor stops spinning Wait until all motion ceases Latch release delay Wait 4 5 s after rotor stops Plunger of latch solenoid is jammed See Section 9 3 and release solenoid plunger Centrifuge vibrates excessively Note momentary vibration is normal during acceleration but should disappear upon reaching full speed Press power button O...

Page 29: ...ll Pop Open Close Firmly and Try Again Won t Spin Power On Yellow Light Unplug Let Yellow Light Bleed Out Turn On Lid May Now Pop Open Latch Solenoid Problem Wait for Rotor to Stop Lid Won t Open Green Light On Or Flashing Noisy Vibrates Remove and Reseat Rotor Cover Don t Overtighten Check Tube Balance Note Momentary vibration rumble is normal during acceleration and deceleration No Yes No Yes ...

Page 30: ...ad Nut Wrench 421292 Rotor Removal Wire 4277 020 006 Line Cords Power Pack United States 421634 Europe 421551 United Kingdom 421554 Power Pack 421763 Operator s Manual 422928 Yellow LED POWER Green LED SPEED Centrifuge Status Operator Action Off Off Power off no rotor motion Plug in Power Pack Flashing Off Ready to spin lid latched Press ON button On Off Power on no rotor motion lid not latched Lo...

Page 31: ... or abuse The warranty stated herein shall extend to the original consumer and not to any subsequent consumer of the centrifuge Drucker Diagnostics shall not be liable for any incidental or consequential damages Drucker Diagnostics makes no other warranties expressed or implied except as stated herein National Contact Drucker Diagnostics 200 Shady Lane Suite 170 Philipsburg PA 16866 U S A Voice 1 ...

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