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4. Click
in
Start run
window or press the button of instrument manual
control and wait until the thermal block is closed.
Caution!
Be sure to place at least 16 tubes in order to prevent their damage because of
hot lid pressure. If there are less of 16 tubes please add empty tubes of the same height
to make them as it is required.
Caution, dangerous!
The instrument provides automatic loading and unloading of
tubes from the thermal block in the operational position. The command to move (open or
close) of the thermal block comes from the operator. It is forbidden to carry out any
manipulations during the movement of the thermal block because it can lead to damage of the
movement mechanisms of it.
If an obstacle appears in the process of the thermal block
closing, it automatically switches to the opening mode.
To prevent the failure of the
movement mechanism in case of a mechanical obstacle occurs during the movement of the
thermal block, the front panel of it is made flexible.
Caution, hot surface!
During the instrument operation, the matrix of thermal block and hot
lid may get hot up to 100 and 105 ºC respectively. When execution of the PCR RUN
(operating mode) the thermal block matrix and the hot lid are not available for operator. Upon
completion of the PCR RUN operator is not allowed to remove the tubes from the thermo
block until the instrument enters in storage mode (low temperature mode) with the following
message:
4.5. Startup of the amplification program
Enter necessary notes such as the particularities and characteristics of the upcoming run and
the PCR mix volume in tubes;
Be sure that the instrument is on and properly connected to the computer, which is confirmed
by yellow
or green
indicator in the software status line.
Start the amplification program by clicking
.
When running the amplification program, select a file name, data format and folder to save
future results of the upcoming RUN.
Note!
If
Run
is started while the instrument is in the process of warming up (yellow indicator
in the software status line), blinking message
Warming
will be appeared
in
Temperature Profile
window:
