DMT 720MO, User Manual

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3 TISSUE BATH MYOGRAPH SYSTEM - 720MO - USER GUIDE
CONTENTS
Chapter 1 - tissue bath overview ..............................................................................................................................................................................................3
Chapter 2 - setting up the tissue bath ..................................................................................................................................................................................4
2.1 Changing and adjusting the mounting supports .............................................................................................................................4
2.1.1 Changing the mounting supports (figure 2.1): ............................................................................................................................4
2.1.2 Fine adjusting the pins for larger vessels (figure 2.2 and 2.3) ...................................................................................................5
2.2 Calibration of the force transducer ..................................................................................................................................................5
Chapter 3 - Experimental set-up ..............................................................................................................................................................................................6
3.1 Mounting protocol ?? ........................................................................................................................................................................6
3.2 Normalization ....................................................................................................................................................................................7
3.2.1 Principles of the normalization procedure ...................................................................................................................................7
3.3 Standard start ....................................................................................................................................................................................8
3.3.1 Principles of the standard start procedure ..................................................................................................................................8
3.4 Endothelium function ........................................................................................................................................................................9
3.4.1 Principles of checking endothelium function ...............................................................................................................................9
3.5 In vitro experiment 1: Noradrenaline contractile response ............................................................................................................9
3.5.1 Background ....................................................................................................................................................................................9
3.6 In vitro experiment 2: Acetylcholine relaxation curve .................................................................................................................. 10
3.6.1 Background ................................................................................................................................................................................. 10
3.5.2 Protocol ........................................................................................................................................................................................ 10
3.6.2 Protocol ........................................................................................................................................................................................ 11
Chapter 4 - CleAning and maintenance ............................................................................................................................................................................. 12
4.1 Cleaning the Tissue Bath ............................................................................................................................................................... 12
4.2 Maintenance of the force transducer ........................................................................................................................................... 13
3.3.1 Checking the force transducer ................................................................................................................................................... 13
4.3.1 Force transducer replacement ................................................................................................................................................... 14
4.4 Maintenance of the linear slides ................................................................................................................................................... 15
Appendix 1 - Buffer recipes ..................................................................................................................................................................................................... 16
Appendix 2 - Normalization theory ...................................................................................................................................................................................... 18
Appendix 3 - Reading a millimetre micrometer ............................................................................................................................................................... 20
Notes ................................................................................................................................................................................................................................................ 21