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                                      Drop / Bubble Counter 

 

 

11 

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

The rate of gas production in an active yeast fermentation of sucrose 

 

 

 
 

1.  Set up the apparatus as shown in the diagram above. Test to make sure bubbles rising 

up in the reservoir will be detected. Attach the Bubble Counter to the 

EASY

SENSE unit. 

2.  Place 40 cm

3

 of active yeast solution (e.g. 0.1% w/v) into the conical flask. 

In this graph the 
end point 
occurred at a 
pH of 4.85 after 
44.7 cm

of acid 

had been added 

End point 

pH 

Volume of 
acid added 

Summary of Contents for SmartQ 3266

Page 1: ...o uk Data Harvest Freely photocopiable for use within the purchasers establishment Drop Bubble Counter Product No 3266 Ranges Drop Bubble Count 0 10 000 23 drops cm3 Volume 0 120 cm3 24 drops cm3 Volu...

Page 2: ...gnal that is counted by the internal counter module The red LED is an indicator which will blink as a drop or bubble passes through the infrared beam There is a small button close to the indicator tha...

Page 3: ...ASYSENSE unit into the hooded socket on the Counter housing Connect the other end of the sensor cable to an input socket on the EASYSENSE unit The red LED will light The EASYSENSE unit will detect tha...

Page 4: ...set the rate of flow and therefore the drop rate from the reservoir The lower stopcock is used in either an open or closed position to turn the drops on and off The line of the blue handle indicates t...

Page 5: ...he body of the reservoir Do not over tighten Check the reservoir is vertical The drops produced will fall through and cut the infrared beam without further adjustment If the reagent reservoir and alig...

Page 6: ...topcocks a and fill the reservoir with the solution being used 2 The first step is to adjust the flow rate Place a beaker under the stopcock to catch the drops Fully open the lower stopcock b Slowly t...

Page 7: ...alibrated for volume but can be used to show the change of gas production over time Test your set up before beginning the experiment It is critical that the column of fluid is positioned so the bubble...

Page 8: ...ment parts pack for the Counter reservoir is available from Data Harvest which contains 2 x 3 way stopcocks and 5 plastic tips use Product No 3271 The Smart Q Drop Bubble Counter works by recording in...

Page 9: ...s used as a Drop Counter with the Smart Q pH sensor to record a pH vs volume graph e g sodium hydroxide with hydrochloric or ethanoic acid ammonia with hydrochloric or ethanoic acid sodium carbonate w...

Page 10: ...o zero 8 Open the EASYSENSE program and select EasyLog from the Home page 9 If the necessary set the Drop Counter to the required range i e Drop Bubble Count or Volume in cm3 e g 27 drops cm3 10 Click...

Page 11: ...diagram above Test to make sure bubbles rising up in the reservoir will be detected Attach the Bubble Counter to the EASYSENSE unit 2 Place 40 cm3 of active yeast solution e g 0 1 w v into the conical...

Page 12: ...be extended to see the effect of different sugar substrates on rate as part of an investigation into respiration glycolysis If a temperature controlled water bath is accessible study the effect of hea...

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