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FineLINE 35 oligo column

Operating Instructions

Original instructions

Summary of Contents for FineLINE 35

Page 1: ...FineLINE 35 oligo column Operating Instructions Original instructions cytiva com...

Page 2: ...6 5 6 Scalingup 27 6 Maintenance 28 6 1 SafetyPrecautions 29 6 2 Usermaintenanceschedule 30 6 3 Cleaning 31 6 4 Storage 32 6 5 Disassemblyandassembly 33 6 6 Componentmaintenance 36 7 Troubleshooting 3...

Page 3: ...boutthismanualandassociateduserdocumenta tion importantuserinformationandintendeduseoftheproduct Inthischapter Section Seepage 1 1 Importantuserinformation 4 1 2 Aboutthismanual 6 1 3 Abbreviations 7...

Page 4: ...agnostic purposes Prerequisites InordertooperateFineLINE35oligocolumnsafelyandaccordingtotheintended purposethefollowingprerequisitesmustbemet Youshouldbeacquaintedwiththeuseofgenerallaboratoryequipme...

Page 5: ...Hardwareitemsareidentifiedinthetextbyboldtext Inelectronicformat referencesinitalicsareclickablehyperlinks 1 Introduction 1 1 Importantuserinformation FineLINE35oligoOperatingInstructions28964957AB 5...

Page 6: ...ntions Softwareitemsareidentifiedinthetextbybolditalictext Hardwareitemsareidentifiedinthetextbyboldtext Inelectronicformat referencesinitalicsareclickablehyperlinks Notesandtips Note A note is used t...

Page 7: ...ETP heightequivalenttoatheoretical plate heightequivalenttoatheoretical plate HIC hydrophobicinteractionchromatog raphy hydrophobicinteractionchromatog raphy PEEK Polyetheretherketone Polyetheretherke...

Page 8: ...cribesemergencyandrecoveryprocedures Inthischapter Section Seepage 2 1 Safetyprecautions 9 2 2 Labelsandsymbols 14 Important WARNING Beforeinstalling operatingormaintainingtheproduct all usersmustread...

Page 9: ...horseriousinjury Itisimportantnottoproceed untilallstatedconditionsaremetandclearlyunderstood CAUTION CAUTIONindicatesahazardoussituationwhich ifnotavoided couldresultinminorormoderateinjury Itisimpor...

Page 10: ...requirements e g tubingwithstandsoperatingpressureandhas adequatechemicalresistance WARNING ThereshouldNEVERbeairorgasunderpressureinthecolumn Flammableliquidsandexplosive environment WARNING Riskasse...

Page 11: ...lasses andotherpersonalprotectiveequipmentappropriateforthe currentapplicationatalltimes Thefollowingpersonalprotective equipmentshouldalwaysbeavailable Protectiveglasses Workingglovestoprotectagainst...

Page 12: ...column espe ciallyifhydraulicsareconnectedtothecolumn Theforcegener atedbythemovementofthecolumncouldcrushhandsandlimbs CAUTION Makesurethatanyaccessoriesusedfulfillthespecification requirements e g t...

Page 13: ...sories Useonlyaccessoriessuppliedorrecommendedby Cytiva WARNING Decontaminatebeforemaintenance Toavoidpersonnelbeing exposedtopotentiallyhazardoussubstances makesurethatthe columnisproperlydecontamina...

Page 14: ...lowingtable Symbol text Description Codeno OrderingnumberforFineLINE35oligocolumn Designpressure Thedesignpressure whichmustneverbeexceeded Warning Readtheuserdocumentationbeforeusingthe system Donoto...

Page 15: ...mn 1 2 3 4 5 6 7 8 9 10 11 Part Description 1 Columntopconnection 2 Adaptershaft 3 Mechanicallockingunit 4 Bayonetadapterlidlock 5 Columntoplid 6 Connectingrod 7 Columntube 8 Adapterfilterunit 9 Botto...

Page 16: ...tainsthebottomfilterunitandstainlesssteel 316L filter andanO ringsealstheunitagainstthecolumntube Materials Allcolumnpartsaremanufacturedfromhighqualitymaterialsandmeettherequire mentsofindustrialenvi...

Page 17: ...ofthecolumnarelocatedatthebottomofthecolumn inthe bottomlid andinthecolumntop Theinletandoutletshave5 16 fittingandthehydraulicchamberhasM6fitting 3 Systemdescription FineLINE35oligoOperatingInstructi...

Page 18: ...ocolumnisdeliveredassembled withtheadapterfittedinthe column Packinglist Item Articleno Qty FineLINE35oligocolumn 28946841 1 Usermanual 28964957 1 Hexkey4mm 59037900 1 Hexkey2 5mm 59444200 1 Union5 16...

Page 19: ...FineLINE35oligocolumninsafe way Inthischapter Section Seepage 5 1 Safetyprecautions 20 5 2 Packingthecolumn 22 5 3 Lekagetest 24 5 4 Recommendedoperatingconditions 25 5 5 Unpackingthecolumn 26 5 6 Sca...

Page 20: ...tsofthecolumntube Thepressurespecificationsareonly validiftheglasstubeisundamaged WARNING Max columnpressure Theworkingpressureofthecolumn shouldneverexceed20bar otherwisethereisariskofpersonal injury...

Page 21: ...akesurethatthepressurereliefvalveiscorrectlyadjustedand thatconnectingtubinghasanunrestrictedflowpath NOTICE Toavoidprecipitationkeepallreagentsatorabove18 C NOTICE Noothersolventsthanthosespecifiedin...

Page 22: ...peratingInstructions 1 Hexkey4mm 1 Union5 16 female M6male 2 TubingConnectorsOD1 8 tubing 12 FerrulesforOD1 8 tubing 12 Stopplug5 16 1 TubingID1 6mmOD1 8 30cm 2 TubingID1 6mmOD1 8 40cm 3 100or250mLbot...

Page 23: ...eredlinesonthe column 6 Wettheadapterwith50 to95 ethanol 7 Connectstopplugtothebottomofthecolumn 8 Connectatubetotheadapterandinserttheotherendsecurelyintoawaste vessel 9 Inserttheadapter Adjustthecol...

Page 24: ...min 2 Doacheckifliquidcomesoutfromthecolumnoroutfromconnection points 3 Ifnoleakageisdetected proceedtostartarun 4 Ifaleakageisdetected a Localizetheleakage b Determinethecauseoftheleakage SeeGeneral...

Page 25: ...ecolumn throughthebottomfilterandexitthroughthetopadapterfilter Simplifyingfilterchanging Usingflowdirectionfrombottomtotopalsosimplifieschangingadirtyadapter filter sincetheinletfiltermostlikelyisdir...

Page 26: ...NG ThereshouldNEVERbeairorgasunderpressureinthe column 4 Continuetheairflowuntilthesupporthasreducedinvolumeandisfree fromthecolumnwall Thiswilltakeapproximately30minutesdependingon theamountofsupport...

Page 27: ...tedlargescaleequipmentandthespecialaspects ofliquidhandlingandprocesscontrolathighvolumetricflowrates Linearflowratesandthenumberofcolumnvolumesusedduringmethoddevelop mentshouldgenerallybethesameasth...

Page 28: ...ntenance shouldbeperformedannuallybyaCytivaserviceengineer orservicepersonnel authorizedbyCytiva Alistofplannedmaintenanceactionsispresentedinthis chapter Inthischapter Section Seepage 6 1 SafetyPreca...

Page 29: ...remaintenance Toavoidpersonnelbeing exposedtopotentiallyhazardoussubstances makesurethatthe columnisproperlydecontaminatedandsanitizedbeforemainte nanceorservice CAUTION Makesurethatanyaccessoriesused...

Page 30: ...aning on page 31 Filtercare RefertoSection 6 2 User maintenance schedule on page 30 Afterevery10 thsynthesis runoronceperyear which evercomesfirst O ringcheck RefertoO ring check and replacement on pa...

Page 31: ...enance schedule on page 30 Allcolumnpartscanbecleanedwiththemostcommonlyusedagents suchasdeter gents ethanol methanolandweakacids Forchemicalresistancetospecialcleaning agents seeSection 8 2 Chemical...

Page 32: ...ordingto Section 6 3 Cleaning on page 31 Letthepartsdrythoroughlyaftercleaning Thecolumnshouldthenbestoreddismantledinadryanddustfreelocation Whentakenintoserviceafterstorage makesurethattheO ringsare...

Page 33: ...andglovesresistanttothesubstancesused Followlocal and ornationalregulationsforsafeoperationandmaintenanceof theproduct CAUTION Afterassembly makesurethatthecolumnistightandnotleaking NOTICE Whenworkin...

Page 34: ...dcounter clockwisefromthelockedpositionlabelled 2 onthetopend piecetoposition 1 asshownintheillustration a Ifneces sary usethesuppliedwrench b 2 Wettheadapterrodwithethanolandpulltheadapterlidtoitsupp...

Page 35: ...he suppliedwrench a 3 Wettheadapterfilterunitandtheadapterrodwithethanol 4 Movetheadapterlid b toitslowerstopposition 5 Inserttheadapterintothecolumntubesothattheslotintheadapterlidis positioneddirect...

Page 36: ...eSection 6 2 User maintenance schedule on page 30 TheFineLINE35oligocolumnisdesignedwithaminimumofcomponents whichfacili tatescleaningandreliableoperation Innormalroutineoperation thecolumnrequires on...

Page 37: ...ntube 3 Tightenthebottomfilter Adapterfilterunitreplacement Step Action 1 Unscrewthetwoscrewsthatholdtheadapterfilterunitandremoveitfrom theadapterrod 2 Gentlypulloutthefilterunit 3 Unscrewtheadaptert...

Page 38: ...above Unscrewthetwoscrews thatsecuretheadapterknobandthecolumninlet 2 Pulloutthetubingfromtheadapterrod Unscrewtheadapterknobfrom thetubingusingtheplasticwrenchsupplied 3 Insertanewtubing OD1 8 235mm...

Page 39: ...flow Iftheback pres sureincreaseisduetoablockageinthecolumn thefollowingproceduresshouldbe employed Problem Check possiblecauses Highbackpressure Columnfiltersblocked ifso cleanorreplace RefertoSectio...

Page 40: ...toryinforma tionandorderinginformation andHealthandSafetyDeclarationformforservice Inthischapter Section Seepage 8 1 Specifications 41 8 2 Chemicalresistance 42 8 3 Serviceandtraining 43 8 4 Recycling...

Page 41: ...r 1 2mm Footprint 250x250mm Netmeshsize 15 m absolute20 m Recommendedbedheight 1to10cm Designpressure 20bar Flowvelocityrange 0to200mL min Note Using Primer Support according to recommended packing pr...

Page 42: ...attheeffectsofachemicalwillbemoresevereathighertempera turesandpressures andthatcombinedeffectshavenotbeentakenintoconsidera tion Concentrationsareexpressedas volumeofsaturatedsolutionunlessotherwise...

Page 43: ...3 Service and training ContactyourCytivarepresentativeregardingserviceandtrainingforFineLINE35oligo column 8 Referenceinformation 8 3 Serviceandtraining FineLINE35oligoOperatingInstructions28964957AB...

Page 44: ...Theproductmustbedecontaminatedbeforedecommissioning Alllocalregulations mustbefollowedwithregardtoscrappingoftheequipment Disposal Whentakingtheproductoutofservice thecolumnshouldbeemptied cleanedand...

Page 45: ...ststheregulationsandstandardsthatapplytotheproduct Inthissection Section Seepage 8 5 1 Contactinformation 46 8 5 2 EuropeanUnionandEuropeanEconomicArea 47 8 Referenceinformation 8 5 Regulatoryinformat...

Page 46: ...Manufacturinginformation Thetablebelowsummarizestherequiredmanufacturinginformation Requirement Information Nameandaddressofmanufacturer CytivaSwedenAB Bj rkgatan30 SE75184Uppsala Sweden Telephonenumb...

Page 47: ...notincludedwiththeproduct acopyoftheEUDeclarationofConformityisavailable onrequest CEmarking TheCEmarkingandthecorrespondingEUDeclarationofConformityisvalidforthe instrumentwhenitis usedaccordingtothe...

Page 48: ...umnsystem Precautions WARNING Accessories Useonlyaccessoriessuppliedorrecommendedby Cytiva CAUTION Makesurethatanyaccessoriesusedfulfillthespecification requirements e g tubingwithstandsoperatingpress...

Page 49: ...ustration It fits between the Adapter Lid 3 and the Adapter tube 6 Thetablebelowdescribescomponentpartsandaccessories Theitemnumbersrefer totheillustrationabove Itemswithoutarticlenumbercannotbeordere...

Page 50: ...lterunit35 PEEK 316L 1 18113716 Yes Yes 17 Bottomendpiece POM 1 No No 18 Adhesivefootpads 4 No No 19 Bottomfilterunit35 PEEK 316L 1 18113717 Yes Yes 20 NutM6 316 3 No No 21 O ring3 6x1 6 EPDM 2 181142...

Page 51: ...Qty Articleno Wetted process Wetted hydraul WrenchFineLine351 1 18111088 WrenchM6 1 19748101 OperatingInstructions 1 28964957 8 Referenceinformation 8 6 Orderinginformation FineLINE35oligoOperatingIns...

Page 52: ...some cases this may require customer to move equipment from normal operating location prior to Cytiva arrival Consumables such as columns or gels have been removed or isolated from the instrument and...

Page 53: ...must be decontaminatedprior to service return Provide a telephone number where Cytiva can contact you for additional information concerning the system equipment Telephone No Liquid and or gas in equip...

Page 54: ...veowners 2020 2021Cytiva UNICORN 2021Cytiva AnyuseofUNICORNissubjecttoCytivaStandardSoftwareEnd UserLicenseAgreementforLifeSciencesSoftwareProducts Acopy ofthisStandardSoftwareEnd UserLicenseAgreement...

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