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ML230 Focused-ultrasonicator

Setup & Instruction Manual

Instrument Developed for High-performance 

 Sample Preparation using Adaptive Focused Acoustics

®

 (AFA

®

) 

For Research Only

Model Number: ML230 | Software: SonoLab 10.1

Covaris

Part Number: 010516 Rev B | March 2020

ML230 Setup & Instruction Manual

Summary of Contents for ML230

Page 1: ...ion Manual Instrument Developed for High performance Sample Preparation using Adaptive Focused Acoustics AFA For Research Only Model Number ML230 Software SonoLab 10 1 Covaris Part Number 010516 Rev B...

Page 2: ...ly Acquired Infections Approved Guideline Third Edition published 2005 ISBN 1 56238 5674 Occupational Safety and Health Administration OSHA 29 CFR 1910 1030 Bloodborne Pathogens International Standard...

Page 3: ...instruments are designed to operate in ambient laboratory conditions e g 19 C to 25 C 66 F to 77 F DO NOT operate the instrument in a cold room environment the system is designed to operate with a wa...

Page 4: ...delivered is free of defects in materials under normal use and service The warranty is void if damage has resulted from third party software not intended for use with the system The high power focused...

Page 5: ...30 Water Control 19 3 4 Loading the Sample 19 3 5 Running a Method 21 3 6 Instrument Light 21 3 7 User Maintenance 22 3 8 Shutdown Procedure 23 4 Using a Pre installed Method 24 4 1 DNA Shearing Proto...

Page 6: ...be used in laboratory environment The instrument is ideal for those who work with DNA shearing and sample preparation The system is comprised of the Treatment System which delivers the acoustic energy...

Page 7: ...y Box Bubble Level Tool Wrench for removing the caps vents from the water bottles Sample Tray Assembly 2 Plastic water bottles 1 ML230 power cord Sample Racks and Consumables depending on order config...

Page 8: ...the instrument 1 Assemble the Water Control supply and waste bottles Locate the two plastic bottles and the bottle cap tool in the accessory box Figure 2 3 Front view of the ML230 instrument Figure 2...

Page 9: ...the front of the ML230 The vented cap is toward the rear the Quick Disconnect cap toward the front of the instrument Slide the Supply bottle filled with water into the remaining opening between the W...

Page 10: ...See Figure 2 4 2 Assemble the Sample Tray Assembly to the ML230 Locate the Sample Tray Assembly in the accessory box Figure 2 7 Bottles into Water Works Bay Figure 2 8 Connecting Water Works Bottle t...

Page 11: ...r open Slide the Sample Tray Assembly locating pin holes onto the pins on the motion system behind the safety cover The sample tray faces to the left Refer to the image below Figure 2 10 Mounting Brac...

Page 12: ...the appropriate assembly steps 2 3 Leveling the ML230 Instrument This step is very important to ensure proper filling and draining of the water bath and proper instrument operation Once the ML230 is...

Page 13: ...to be shortened If the bubble is to the left side the right side is low and the right side feet must be lengthened Adjust both front and rear feet an equal number of turns Repeat the process above to...

Page 14: ...a window will appear asking whether the instrument is to be homed Touch Yes The motion system will perform its initialization sequence the homing window will close and the SonoLab Home screen will be...

Page 15: ...during acoustic treatments The 2 axis Motion System is designed to automatically align the sample tube at the appropriate position and height in the acoustic field for processing Tubes are loaded into...

Page 16: ...place on the ML230 sample tray See Section 3 5 Loading the Samples d Water Temperature must be within acceptable Min and Max limits e Water must be at the level required by the consumables specified...

Page 17: ...he method temperature setpoint after Run is selected Water Temperature status will turn green when temperature is within the Min and Max limits typically 3 degrees above or below the setpoint The defa...

Page 18: ...box If a condition is not acceptable the status will show X If any of the items in the Status list display X a red X will also be displayed in the System Status button as shown in Figure 3 6 Once a m...

Page 19: ...Quick Disconnect Cap as shown in Figure 3 7 to release the Quick Disconnect Insert of the bottle being serviced and slide the bottle out Use the Water Bottle Cap tool to remove the vented cap at the r...

Page 20: ...ion Open the ML230 safety cover Place tubes and rack assembly into the ML230 sample tray shown in Figure 3 10 The racks are keyed to load into the sample tray in the correct orientation only Close the...

Page 21: ...any time while running a method touch Pause to pause a running method The Pause button becomes a green Resume button When the method is paused the user may continue the process by touching the Resume...

Page 22: ...ml of household bleach or equivalent in the Water Control Supply bottle and verify the Waste bottle is not full Touch the Water Control button to display the Water Control panel Touch Fill and wait f...

Page 23: ...dure If the instrument will be idle for an extended time e g overnight or a weekend shut down the instrument using the following procedure Empty the water bath and store the sample rack in a clean dry...

Page 24: ...4 1 DNA Shearing Protocols DNA Shearing protocols are optimized for the mean DNA fragments size sample volume and sample vessel used These protocols are stored in a folder called DNA Shearing for ML2...

Page 25: ...ny times as necessary A subset of treatments within a Process may also be repeated If all the steps of the Process are to be repeated a global Repeat step is placed outside the process See Section 5 1...

Page 26: ...method from the Plate Definition drop down list 5 1 1 Add a Step in the Method Touch the New button The Choose a Step Type pop up is displayed Touch Process The process bar is displayed If steps are t...

Page 27: ...hosen The product of the Peak Power and Duty Factor gives the approximate Average Incident Power AIP generated from transducer For example if Peak Incident Power is set to 10 Watts and Duty Factor is...

Page 28: ...motion capability in the Y axis left to right If a treatment specifies dithering touch the Pen Paper icon on the right end of the Process bar to display the Dithering Parameters pane Enter the specif...

Page 29: ...e by touching the pen paper button of the prompt bar to display the text entry field for the prompt Enter the text of the prompt and touch OK See Figure 5 7 When the prompt step is reached during the...

Page 30: ...p and next delay step in the same manner Please contact applicationsupport covaris com for inquiries regarding the tepeat pulsing feature and method setup 5 1 6 Saving Method When all method parameter...

Page 31: ...Number 010516 Rev B March 2020 ML230 Setup Instruction Manual Make the desired changes to the method then save as described above Figure 5 10 Selecting a Method to Edit Select a method to edit here t...

Page 32: ...reen The Method Screen Figure 6 1 is the principal user screen for the system Section 3 contains details on operating the instrument through the Method Screen The right side of the Method Screen displ...

Page 33: ...o be scanned by the RFID reader Stop stops any current motion Touch the Water Control button to display the Water Control pane The Water Control pane displays the current temperature level and degassi...

Page 34: ...n the event that the bath water is degassed but SonoLab has been restarted which causes the degas timer to start over 6 2 The Schedule Tab The Schedule screen is used to configure bath Auto Fill and s...

Page 35: ...EADY and OFF READY is for configuring a time for the system to be ready to run typically ahead of a daily plant startup in the morning OFF is for placing the system in an idle state typically at the e...

Page 36: ...ethod was run the operator who ran the method or the run status complete aborted failed Double tap on a line to display its details The left side of the screen will display textual information concern...

Page 37: ...ion regarding the method run is displayed above the graphics See Figure 6 4 6 4 The About Tab The About tab provides user basic information about the instrument such as serial number software version...

Page 38: ...ion requires no login to create run and edit methods User Authentication may be enabled to limit access to SonoLab functionality To enable User Authentication Start SonoLab Touch the Log In button at...

Page 39: ...s to Administrators and Users Can run and edit any methods installed on the system An Editor Can run and edit methods assigned to them by an Administrator Can create methods Can edit and run methods c...

Page 40: ...er 010516 Rev B March 2020 ML230 Setup Instruction Manual Enter a new Username the user s first and last names and touch the Assigned button By default all treatment methods on the system will be assi...

Page 41: ...simultaneously Touch the RIGHT ARROW button The methods to be removed are moved to the box at the right Touch SAVE to save changes and exit the New User screen The new user now appears in the list of...

Page 42: ...duct Safety WEEE Safety Certified to IEC EN ANSI UL61010 1 and CAN CSA C22 2 No 61010 1 Safety Requirements for Electrical Equipment for Measurement Control and Laboratory Use Part 1 General Requireme...

Page 43: ...ottle is properly connected to the Quick Disconnect Cap see Section 3 There could be a blockage in the drain lines Ensure the bath is clean and free of debris This may be resolved with a bath cleaning...

Page 44: ...r Control button and selecting Empty Remove the sample tray by unscrewing the captive thumb screw securing it to the motion system and gently pulling it forward off the locating pins Pack the sample t...

Page 45: ...ent should be replaced annually 1 Disconnect the Supply and Waste bottles by pressing the thumb latch on the Quick Disconnect Fittings and lifting them out of the bottles See Figure C1 2 Remove the Su...

Page 46: ...s the upper water connector into the female fitting of the Particle Filter Element until it clicks into place 8 Gently press the lower fitting of the Particulate Filter Element into the lower water fi...

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