If you with to store gels you have prepared. Be sure to cover the gel with buffer. Store the gel at a
low temperature
7. _Holding the vertical portions of the plate remove the plate containing the gel from the stand.
8. _Place the gel casting plate on the gel bed in the centre of the electrophoresis tank.
9. _Place the gel casting plate in such way that the negatively charged DNA migrate in the
direction of the “+” marked on the electrophoresis tank (opposite the connector).
10. _Pour buffer in the electrophoresis tank to a level slightly (3 to 5 mm) above the gel surface.
It is important to keep the gel unit clean, particularly the gel-casting plate, and the combs. Do not
clean any part of the gel unit with organic solvents such as ethanol or acetone.
3. Power Supply Operation
Check the Quick Start Operation Manual.
1. _Insert the power supply connector into the electrophoresis tank.
5
Power switch
Power code
3. _Set the comb. Select a comb with the desired number of teeth.
4. _Allow the agarose to cool below 70°C before pouring.
5. _Pour the gel. For 4 mm thick gels, about 80 ml of agarose is required.
6. _After the gel has solidified completely, pour the buffer solution onto the gel to a level just
above the gel surface and gently draw out the comb.
Insert the comb into the hole to which the A
mark to the right of the gel casting tray is
attached if you need the same six-lane distance.
Insert the comb into the hole to which the B
mark to the left of the gel casting tray is
attached if you need the same four-lane
distance.
MyRunN/manual 04.10.28 10:48 AM ページ7
