CORNING Spin-X UF 6, Technical Data And Operating Instructions

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2.
Prerinsing
Membranes fitted to Spin-X
®
UF concentrators contain trace amounts of glycerin and
sodium azide. Should these interfere with analysis they can be removed by rinsing fill
volume of buffer solution or deionized water through the concentrator. Decant filtrate
and concentrate before processing sample solution. If you do not want to use the pre-
rinsed device immediately, store it in the refrigerator with buffer or water covering
the membrane surface. Please do not allow the membrane to dry out.
3.
Sterilization of Polyethersulfone Membranes
Polyethersulfone membranes should not be autoclaved as high temperatures will
substantially increase membrane MWCO. To sterilize, use a 70% ethanol solution
or sterilizing gas mixture.
Technical Specifications
Table
1
. Technical Properties
Spin-X UF 6 Spin-X UF 20
Concentrator Capacity
Swing bucket rotor 6 mL 20 mL
Fixed angle rotor 6 mL 14 mL
Dimensions
Total length 122 mm 116 mm
Width 17 mm 30 mm
Active membrane area 2.5 cm
2
6.0 cm
2
Hold up volume of membrane <10 µL <20 µL
Dead stop volume* 30 µL 50 µL
Materials of Construction
Body Polycarbonate Polycarbonate
Filtrate vessel Polycarbonate Polycarbonate
Concentrator cap Polypropylene Polypropylene
Membrane Polyethersulfone Polyethersulfone
*Dead stop volume as designed in molding tool. This volume may vary depending on sample, sample
concentration, operation temperature and centrifuge rotor.
Table
2
. Maximum Recommended Spin Force (xg)
Swing Bucket Fixed Angle
Spin-X ® UF 6
5,000 to 50,000 MWCO PES 4,000 xg 10,000 xg
100,000 MWCO PES 4,000 xg 6,000 xg
Spin-X UF 20
5,000 to 50,000 MWCO PES 5,000 xg 8,000 xg
100,000 MWCO PES 3,000 xg 6,000 xg
Table
3
a. Performance Characteristics Spin-X UF 6
(Time to concentrate up to
30
x [min.] at
20
°C and solute recovery %)
Rotor Swing Bucket 25° Fixed Angle
Start Volume 6 mL 6 mL
Min. Rec. Min. Rec.
BSA 1.0 mg/mL (66,000 MW)
5,000 MWCO PES 20 98% 12 98%
10,000 MWCO PES 13 98% 10 98%
30,000 MWCO PES 12 98% 9 97%
IgG 0.25 mg/mL (160,000 MW)
30,000 MWCO PES 18 96% 15 95%
50,000 MWCO PES 17 96% 14 95%
100,000 MWCO PES 15 91% 12 91%
Table
3
b. Performance Characteristics Spin-X UF 20
(Time to concentrate up to
30
x [min.] at
20
°C and solute recovery %)
Rotor Swing Bucket 25° Fixed Angle
Start Volume 20 mL 14 mL
Min. Rec. Min. Rec.
BSA 1.0 mg/mL (66,000 MW)
5,000 MWCO PES 23 99% 29 99%
10,000 MWCO PES 16 98% 17 98%
30,000 MWCO PES 13 98% 15 98%
IgG 0.25 mg/mL (160,000 MW)
30,000 MWCO PES 27 97% 20 95%
50,000 MWCO PES 27 96% 22 95%
100,000 MWCO PES 25 91% 20 90%