Creating User Protocols
By pressing the Plate Filling, Serial Dilution, or Plate-to-Plate
Transfer button in the main menu, the following screen will appear:
General Settings Tab
You can edit the following to customize your protocols:
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Protocol name
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Liquid handling speed
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Link file option: Allows you to select a protocol to be run after
the current one is finished (link multiple protocols)
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Tip rack orientation
◗
Tip change frequency
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Which tips are used: The plate interface can be interacted with
to determine which rows of tips are used (green = selected)
Protocol Settings Tab
Depending on the protocol, you can edit the following:
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Source plate location
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Source plate volume
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Destination plate location
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Destination plate volume
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Transfer volume
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Blowout volume
◗
Number of mixes in plate
◗
Source and destination
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Plates (serial dilution)
◗
Mix volume (serial dilution)
You can also interact with the plate diagram on the right and
modify the rows filled on the destination plate. When you
are finished customizing your protocol, return to the General
Settings tab and select Run in the upper right corner.
NOTE:
Be sure to confirm the tip rack and plates/reservoirs are in
the correct position before pressing Run.
Running User Protocols
After selecting Run, the instrument will begin performing the
selected protocol (Plate Filling, Serial Dilution, or Plate-to-Plate
Transfer).
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During each protocol, the instrument will track the status
of each operation performed. You can save your protocol by
selecting More Options and then Save User Program.
◗
At any time the instrument is running, you can press the Stop
button to pause the instrument. You will then have the option
to either abort or continue the protocol.
Cherry Picking Protocols for Single channel
By pressing the Single channel button in the main menu, the
following screen will appear:
General Settings Tab
In the General Settings tab, you can edit the following:
◗
Magazine tips
◗
Source plates
◗
Destination plates
NOTE:
You will need to assign at least one magazine tip, source
plate, and destination plate to run a protocol. However, up to
3 different tip racks/plates can be assigned for more complex
protocols.
