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GenoSens Series

Gel Documenta�on System

MANUAL

Clinx Science Instruments Co., Ltd.

[email protected]

5C-102, 258 West Songxing Road., Shanghai, China

www.clinxsci.com

Summary of Contents for GenoSens Series

Page 1: ...GenoSens Series Gel Documenta on System MANUAL Clinx Science Instruments Co Ltd info clinxsci com 5C 102 258 West Songxing Road Shanghai China www clinxsci com ...

Page 2: ...Contents Important no ce 01 Product introduc on 05 Hardware guide 09 So ware installa on 15 Gel Capture 21 Gel Obsera on 37 Analysis so ware 41 Warrenty Liability 57 ...

Page 3: ...01 Important No ce ...

Page 4: ...leave the instrument in a damp dusty or hot place Do not pour liquids or inside the instrument Do not drag or scratch with hard or sharp objects to prevent scratches Read the user guide carefully The instrument is suitable for research use only Therefore it must be used onlyby specialized personnel who know the health risks associated with the reagents that are normally used with instrument 02 Saf...

Page 5: ...ensive light when the p ower is on Note 1 If the blue light of power is on the UV works Note2 Using UV shield is a must when you need to observe or to cut gel Note3 Wearing the goggles for protec on of your eyes away from the blue light illumina on when your need to observe the gel with blue light transilluminator 04 ...

Page 6: ...o captu re u er weak signals under extremely low illumina on condi on Summer y Panel Components Bu ons Epi White LED upper RGB Fluorescence LED UV Transilluminator USB ports Epi White LED lower GenoSens 2000 Series front panel components GenoSens 2000 Series side Panel Components GenoSens Touch Series front panel components Touch Screen Epi White LED upper RGB Fluorescence LED Blue White LED Trans...

Page 7: ...Power switch USB 3 0 port 08 The back sides of these two series keep consistent GenoSens Touch Series side panel components USB Ports Epi White LED lower Opera on mode converter 03 Hardware Guide ...

Page 8: ...t your provider im mediately According to your op on we provide the following accessories Op onal Accessories 1 2 1 Ultra slim white LED transilluminator 2 Ultra slim blue LED transilluminator Configura on and applica on list Configura on Applica on Main System Gel Documenta on with EtBr etc White LED Transilluminator SDS PAGE Silver staining etc Blue LED Transilluminator Gel Documenta on with Gel G...

Page 9: ...r if you want to use the stand alone PC Power switch and USB port are shown as below 12 InstallationofsuperslimTranswhite bluesamplestage The ultra thin blue light sample stage has a magnetic thimble port The side of the magnetic thimble port should be aimed at the inside of the instrument and push the blue light stage into the drawer Position is as shown Magnetic thimble 13 13 ...

Page 10: ... To power the sample stage on by touching the power icon 2 Brightness adjustment Based on indica on of brightness in the pi cture you can adjust brightness by touching and dragging The inten sity of white and blue light is increased from the le to right Power Intensity adjustment 04 So ware Installa on 14 ...

Page 11: ...n system All the intelligence property of the so ware men oned below belongs to Clinx Science Inst ruments Co Ltd We promise we offer free life me upgrades Installa on guide Double click the installa on so ware package Select language Click Next 16 17 GenoSens Capture PC version GenoSens Capture Touch version ...

Page 12: ...Select the installa on path and click Next It is recommended to choose the default op on Click Install to proceed Click install to con nue and trust our so ware Installa on guide Installa on guide 18 19 ...

Page 13: ...nd the installa on is completed So ware icon 05 Gel Capture Clinx GenoSens Capture so ware Clinx GenoSens Analysis so ware Two icons will be generated on the desktop a er the so ware installa oin Installa on guide 20 ...

Page 14: ... instrument Users should be trained on the appropriate personnel protec on gear for working with UV light to minimize UV exposure Safety alert Part 1 Gel Capture PC standalone version Run GenoSens Capture func on 1 2 3 4 5 No Description 1 Tool Bar 2 Preview window 3 Parameter setting 4 Display setting 5 Auto save path Please refer to page 13 and 14 in the manual Super Slim transilluminator Instal...

Page 15: ...w Press the le mouse bu on and select area you want to crop as shown on the le Press the Enter to complete image cropping as shown on the le Click icon to enter the cropping mode Cancel image processing and restore the original image by clicking Tips The image processing func on can only be used when you have captured the image Please refer to P29 30 in this chapter about image acquisi on 111 24 2...

Page 16: ...n alternately to power the Super Slim trans white trans blue LED transilluminator on or off White LED Transilluminator apply for the Protein gel Blue LED Transilluminator apply for the nucleic acid gel with dyes such as Gel Green and Sybr Green Blue LED white LED Transilluminator Installa on refers to p13 p14 in user manual UV Transilluminator Click icon alternately to power the Super Slim trans UV...

Page 17: ...resolu on for shoo ng the system will provide high defini on images The experimental image can be used to print HD files Note The exposure me will be longer if you choose High resolu on mode The sensi vity of the camera will decrease When you select Low resolu on for shoo ng the system will provide low defini on images Note The exposure me will be shorter if you choose Low resolu on mode The sensi vi...

Page 18: ...djust the grayscale range Auto save file path Click this icon to select folder path which is automatically saved shown as below It will be automa cally saved into this folder when picture is taken in every me and the customized file name the date and me of the shoo ng will be displayed shown as below Turn on off the preview mode The image will be present ed based on parameters such as aperture light ...

Page 19: ...ture Tool bar 4 Status bar Shooting parameters setting Images save and edition Language option Main interface of Touch screen opera on Shooting parameters setting Click this icon to set the shoo ng parameters including light source fluorescence filter wheel lens and acquisi on se ngs A Light source se ng Click the icon shown on the le to open the light source Introduc ons to the light source please ...

Page 20: ... to P26 P27 in this chapter Click this icon to edit the image and save the picture into the spe cified path Note Before edi ng and saving an image we firstly need to capt ure the image A Display Se ng A er the image is acquired we can add pseudo color through the disp lay se ngs To adjust the grayscale value low value High value you can get the best picture to be watched Instr uc ons on adjus ng the...

Page 21: ...Status Bar 36 核 酸 胶 拍 摄 The status bar displays the key status when the instrument runs If you want to know the detailed informa on please refer to P26 in this chapter 06 Gel observation 36 ...

Page 22: ...pening Please read safety warnings on page P3 P4 of the manual if you need to cut gel recovery A Turn the UV translluminator on by pressing panel If you are using the ChemiScope 6000 Series System you can turn on the UV transilluminator by pressing UV transmission bu on on the front panel of the inst rument as shown on the le B Turn the UV translluminator on by software If you are using the ChemiS...

Page 23: ...or No need to turn on blue white LED Transilluminator through the so ware For details please refer to P14 in manual Blue and white light and other types of visible light are exposed to eyes that can cause harm When using blue and white light transmission sta ons Please be sure to protect your eyes when using blue white LED Transilluminator For details please refer to P4 in manual 40 ...

Page 24: ...window 5 Bands window 6 Adjustment of Grayscale range window No Descrip on Menu Bar File Menu Image Menu 1 2 3 4 5 6 Gray scale display setting Before we start analyzing the image we need to adjust grayscale range so that the background and the brightness of bands are the best situa on Firstly we switch the High and Low values in the le corner of the so ware to manual state as shown below The Low ...

Page 25: ...unc on for Image menu bar Image cropping A er click the Image cropping the menu bar Cropping will be cked Short cut of the crop will be ac ve Hold the le mouse bu on and select area you want to keep as shown on the le A er selec ng press the Enter key to co mplete the cropping of image as shown on the le 45 44 ...

Page 26: ...picture is shown on the figure above The bands basically kept in a horizontal posi on for later analysis Main func on for Image menu bar Ver cal rota on When the bands of the original image is not horizontal as sh own on the le We can also use this func on to put bands horizontally for image analysis A er click this func on we can use the mouse to pull a ver cal line along the edge of the bands Adj...

Page 27: ... page 51 Image Rota on Refer to Page 50 D Auto lane detec on E Manual lane configura on Click the icon to ac vate Manual lane configura on and enter number in the lanes then define the percent Percent means the propor on of the space occupied by bands If the percent is 90 10 is the distance between the lanes The percent is generally set to 90 Click the icon to ac vate Auto lane detec on and hold le m...

Page 28: ...lect the lane and use the le mouse b u on to click the band you want to delete in the strip win dow G Molecular weight Se ng molecular weight Mark the molecular weight of each band according to the Marker instruc on As shown above we first select lane in the area and iden fy the bands refer to P55 P58 Click the le mouse bu on to select the standard lane in the yellow box Click to open the wind ow o...

Page 29: ...0 from top to bo om You can save theseconfigura on into the file as format cmr by Save As bu on in order to use it in the next me without inpu ng the same molecu lar weight again Click Open bu on to import former co nfigura on file II Input molecular weight of Marker The Data window introduc ons please refer to P61 in this chapter I Choose Marker lane A er you enter the molecular weight the bands corr...

Page 30: ...are can open mul ple image files and they are displayed as thumbnails We can select the images to be analyzed by clicking the thumbn ail as shown below Once the band is recognized the thumbnail window will automa cally switch to the data window You can also switch manually by clicking the Thumbnail or Analysis Report tab on the le Right click on the data window area you can open the Molecular Weigh...

Page 31: ...Data Presen on Save the analysis report as an Excel file by right clicking on the data window 08 Warranty Liablity 56 ...

Page 32: ...ca ons It must be installed and used with the manuals provided by our company We provide one year warranty without the external force or man made damage Improper use or wrong opera on result in the loss of the income or business opportunity that is not within the respo nsibility of our company Warranty Liability 58 Address 5C 102 258 West Songxing Road Baoshan District Email info clinxsci com ...

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