Endosafe
®
-
Portable Test System User’s Guide
Copyright 2003
Page D2
PIPTS101-10
x
Enter Sample ID
(Selecting and scrolling with the menu key under the
sample ID Header allows for fifty (50) samples to be entered and
stored)
x
Enter Dilution Factor
x
Note:
All LAL Reagent Water (LRW) used to make product dilutions
should be tested for the absence of detectable endotoxin and glucan.
While the above information is being entered into the reader, the cartridge
is being pre-warmed.
1.
Dispense the sample
Once all test information is entered, the reader
displays:
x
ADD SAMPLE; PRESS ENTER
x
Pipette
25
μ
L of sample into four (4) sample
reservoirs of the inserted cartridge and press
Enter
on the reader keypad
x
Pumps draw sample aliquots into the test
channels, thereby initiating the test
x
Results will be obtained in approximately 30 minutes.
GLUCAN TEST RESULTS
1. When the test is complete, the PTS Reader gives an audible notification
that the assay is finished.
2. At the conclusion of the test, the glucan measurement and the assay
acceptance criteria are displayed on the screen.
3. The reader display alternates between the following results:
x
Sample
pg/mL
Value
x
Sample
%CV
x
Spike pg/mL Value
x
Spike
%CV
x
% Spike Recovery
x
Remove
Cartridge
The reader continues to display the assay results until cartridge is removed.
Once the assay is complete and the results are noted, remove the cartridge
promptly from the reader.
