Evaporation Procedure
1.
Set the temperature on the dry block heater below the most volatile analyte to
ensure that no analytes are lost during evaporation. Locate the VWR instruction
manual (P/N 549040-00) for instructions on setting the heater.
2.
Once temperature has stabilized, raise the nitrogen manifold by twisting the black
knob counterclockwise and lift to an appropriate height to permit loading of the
vials.
3.
Twist clockwise to lock the manifold in place.
4.
Load the uncapped vial(s) into the metal block, being careful to avoid prolonged
direct contact with the heated metal block. Collections vials can be purchased from
CEM Corporation or through its authorized dealer network.
• P/N 153450 - 40 mL Clear Glass Graduated Vials, Pack of 72
• P/N 153455 - 40 mL Amber Glass Vials, Pack of 72
• P/N 153300 - 40 mL Clear Glass Vials, Pack of 72
• P/N 153700 - 60 mL Clear Glass Collection Vials, Pack of 72
• P/N 153705 - 60 mL Amber Glass Collection Vials, Pack of 72
5.
Lower the manifold into place using the black knob until the nozzles are inside the
vials.
6.
Turn the nut located on top of the nitrogen manifold counter-clockwise to allow the
flow of nitrogen for the desired position(s). Close all positions that are not in use.
7.
Turn on the nitrogen flow using the black knob located on the flowmeter. Ensure
that the flow is low enough to prevent splashing out of the vials but high enough to
increase the evaporation rate.
NOTE
The pressure regulator has been set to 80 psi to ensure the safety of the user
and the longevity of the evaporator; therefore, the pressure regulator should
never be adjusted.
8.
Once evaporation has completed, close all positions on the manifold and remove
the vials as outlined in the steps above.
Flow Rates
Number of Vials
40 mL Vial(s)
60 mL Vial(s)
1-6
< 40 SCFH
< 80 SCFH
7-12
< 80 SCFH
< 140 SCFH
SCFH = Standard Cubic Feet per Hour
• Start with a low flow to ensure that there is no risk of splashing of extract.
• Increase flow as needed by 20 SCFH every 10 minutes to speed up the rate of
evaporation.
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