CellScale MechanoCulture TM, User Manual

Page: 18 / 27

MechanoCulture TM User Manual
14
6.
Setting Up & Running a Test
Overview
As a user of the MechanoCulture, you are likely to develop your own test preparation and
execution protocol, but there are several elements that are common to most users. This manual
will not cover cleaning, disinfecting, sterilization, cell seeding, reagents, and a number of other
topics.
The protocol below should be used for running new test sequences. To re-run existing sequences
on new specimens, simply power-up the device, ensure the system has been reset, mount
specimens, and press the push button to execute the test.
Attach the Sterilized Membrane to the Test Device
See Appendix C for details and images.
Plug in Control Box Power. Connect Control Box and Test
System
Program the Device (if needed)
Connect the Device to the PC via USB
Launch the MechanoCulture Software
Generate a Test Sequence
Upload Test Sequence to Device
Reset the system (hold down the button for 5 seconds)
See comments in Section 4 for details on actuator reset, actuator offset, and well plate pre-
stretch.
Add Cell Media and Cells. Culture to Develop Adhesion
Be sure to set the well plate/carrier assembly on a clean surface so as not to smudge the exterior
of the membrane.
Execute Test
To execute the test sequence, simply press the push button once. To pause the sequence, press
the button again at any point. Once this has been accomplished, power can be removed and
then restored without affecting the test protocol. To restart the test, press the button once more.
To reset the device, press and hold the button for 5 seconds.