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CellScale MechanoCulture TM, User Manual

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CellScale MechanoCulture TM User Manual Download Page 1

 

 

 

 

 
MechanoCulture TM

 

Mechanical Stimulation System

 

 
 
User Manual  
version 1.1 

 

 

 

 

Summary of Contents for MechanoCulture TM

Page 1: ...MechanoCulture TM Mechanical Stimulation System User Manual version 1 1...

Page 2: ...zed for high throughput real time imaging and 3D scaffold use In addition to our standard products CellScale can also develop a custom solution for your application Our products are used by researcher...

Page 3: ...he Specified Test Sequence 6 Well Plate Pre Stretch Device Reset Movement and Offset Distance 7 Offset and Jog Functions 8 5 Designing Test Sequences 9 Example 1 Continuous Cycling for Fixed Duration...

Page 4: ...MechanoCulture TM User Manual 10 Appendix C System Assembly 22...

Page 5: ...quired between uses See Appendix C for assembly instructions Connections to Supply Connect power cord into properly grounded 100 240VAC power source to ensure safe operation The mains supply voltage f...

Page 6: ...MechanoCulture TM User Manual 2 2 Components Overview Power Input Port 3 5mm TRRS Cable Jack Start Stop Reset Button Control Box Mini USB Port...

Page 7: ...m demonstrates each application and release of the well plate is called a test cycle The same test cycle can be repeated multiple times to achieve a certain goal This is called a test set Finally a te...

Page 8: ...given duration Recovering The recovery phase is the time during which the deformation imposed during the stretching phase is removed Resting Finally the rest phase is the time between the end of one...

Page 9: ...l File tmt From the File menu select Open or press the Open button on the toolbar 2 A currently programmed test sequence on a device can be read From the Hardware menu select Read or press the Read bu...

Page 10: ...ck boxes allow for the stretch or recover phases to be omitted during a testing cycle This makes the creation of test sequences that include preloading possible Step 3 Program a Device with the Specif...

Page 11: ...Within the dialogue the offset parameter can be specified and programmed to the device controller This offset will be applied globally to all test protocols once the Program button is clicked As a re...

Page 12: ...ulture TM is programmable by the user and how to do this is shown later in the manual The jog buttons let you extend and retract the actuator outside of executing a test When the actuator is jogged th...

Page 13: ...Then double click on that row to modify it 0 5 Hz is a period of 2 s In this example the period is divided into 1 s of stretch and 1 s of recovery with no hold or rest To achieve a 24 hr test duratio...

Page 14: ...MechanoCulture TM User Manual 10 The resulting actuator motion versus time graph for the first 30 seconds 0 0 5 1 1 5 2 2 5 3 0 5 10 15 20 25 30 Displacement mm Time S...

Page 15: ...modify it Check the Do Not Recover box to omit the recovery phase 2 Press Insert Below to create a second set Modify this set as follows Note that the 5 mm stretch magnitude is relative to the curren...

Page 16: ...MechanoCulture TM User Manual 12 The resulting actuator motion versus time graph for the first 30 minutes 0 0 5 1 1 5 2 2 5 3 0 5 10 15 20 25 30 Displacement mm Time S...

Page 17: ...minutes followed by 50minutes of rest Repeat this pattern indefinitely 1 Press Insert Above to create a Set Then double click on that row to modify it 2 Press Insert Below to create a second set 3 In...

Page 18: ...n Control Box Power Connect Control Box and Test System Program the Device if needed Connect the Device to the PC via USB Launch the MechanoCulture Software Generate a Test Sequence Upload Test Sequen...

Page 19: ...button down for 5 seconds it will fully extend the actuator and retract it to the defined offset amount Actuator Travel Soft Limit The actuator travel soft limit sets the maximum offset that can be a...

Page 20: ...echanoCulture connected to the PC and turned on launch the firmware update software located in the Windows start menu under MechanoCulture Utilities 2 Load the firmware file using the Load Update File...

Page 21: ...to program your MechanoCulture devices To install the software go to the provided web address and download the compressed software package Transfer this file to the computer which will be programming...

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Page 26: ...M system for use 1 Cut the membrane material to 2 5 wide to the desired length 100mm for the default specimen and gather the following tools a Mounting bar b Loading rings c Zip ties d Zip tie tool 2...

Page 27: ...loading rings from the mounting bar and attach the rings to the main device The photos below show the use of the loading rings 5 Plug in the controller and main unit and reset the actuator 6 Mount the...

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