Application hints
Instruction Manual, Feb-17
13
6
Application hints
6.1
Practical procedure for elution
Installation
The TLC-MS Interface 2 can be connected to any brand of MS.
The type of MS is mainly defined by the tasks of your laboratory and by the availability.
For simple tasks, like confirmation of a structure or mass, an easy to use single quadrupol
MS is adequate. For more complex MS/MS tasks (or if it is the only available instrument) a
triple quadrupol MS, or an ion trap MS may be suitable. For identification of unknowns
even a time of flight (TOF), Q/TOF or an Orbitrap MS can be used.
Eluent delivery
For the delivery of the eluent, a HPLC pump is needed. You may use the internal pump of
the HPLC-MS. Remove the HPLC column if installed and connect the respective capillary
from the TLC-MS Interface 2 to the solvent delivery capillary of the HPLC (as described in
this manual).
You may alternatively use a stand-alone HPLC pump.
Connecting the TLC-MS Interface 2 to the MS
Connect the capillary from the HPLC pump to port 2 of the valve and port 1 directly to the
MS (it is recommended to bypass the UV detector cell, because it is sensitive to back
pressure from the TLC-MS Interface 2).
It is highly recommended to use an inlet filter before the MS. The inlet filter is a standard
feature of most MS. For more information check chapter 2.2 Installation environment.
Setting up the MS
Select the ionization mode according to substance requirements and availability: ESI, APCI
or APPI ionization, select appropriate mode (positive or negative).
Tune the MS according to the MS manufacturer’s recommendations.
Optimizing MS parameters
Ideally the substance to be investigated is available in pure form. If not, another substance
with similar properties can be used to verify the ionization conditions.
Eluents are often mixtures of acetonitrile or methanol with water. Mixtures of 80 %
organic phase with 20 % of water have been successfully used. Depending on the nature
of the substances to be investigated, protonation can be enforced by adding 0.1 % of
formic or acetic acid. Deprotonation can be enforced by adding 0.1 % of ammonia.
A flow of 0.2 mL/min is suitable for the elution.
Caution: Do not use any nonvolatile buffer salts (e.g. phosphates) in the eluent and use
only MS grade solvents!
Summary of Contents for TLC-MS INTERFACE 2
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