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Observations & likely cause
• Two channels are non-parallel
• If the incorrect controls have been used to separate the two images, they appear non-parallel.
• when the two channels are superimposed, the only controls that will need adjusting are the
‘Split’ control and the aperture size.
• In the incorrect image below, V1 and V2 have been used to separate the two images in the
vertical axis.
• A quick way to identify this error is if the ‘V’ control moves an image horizontally, which
indicates the camera is in the incorrect orientation.
6) Two emission channels are non-parallel
Resolution
• Refer to the OptoSplit II Set up Guide to familiarise yourself with key controls and follow
‘Adjusting the Position of the Images’ to re-align the two channels.
• It is advisable to initially set up the two OptoSplit channels using the calibration cube included
as standard with your unit. This consists of a 50%/50% beam-splitter, therefore both images will
contain the same information and intensity.
• It is also advisable to refer to the ‘Flowchart for confirming camera orientation’ in section 3 of
this Troubleshooting Guide.
