Using wet ice at 0.5
o
C to 4
o
C
When conventional ice cooling is needed, fill the CoolBox 30 base with crushed ice. It is fine to
slightly overfill the base as the CoolRack module will settle quickly during initial cool down. Place
a room temperature or pre-chilled (4°C) CoolRack on top of the crushed ice. Melting ice and ice-
water will absorb heat from the CoolRack module and will continue to cool until all ice has melted
- up to approximately 10 hours with lid closed.
Using -20
o
C Gel Cartridge (Green) at -20
o
C to -4
o
C
Freeze green -12°C cartridges in a -20°C freezer for a minimum of 6 hours. Pre-chill the CoolRack
module in the -20°C freezer for approximately one-half hour. Place a frozen cartridge in the CoolBox
30 followed by the pre-chilled CoolRack module.
For cooling beyond 6 hours, simply exchange the cooling cartridge with a recharged one.
Using Dry Ice at -78
o
C
Fill the CoolBox 30 base with pulverized dry ice up to level with the bottom of the finger
grip recesses as shown. Place the CoolRack module directly onto dry ice. CoolRack module
temperatures will quickly reach dry ice temperature in approximately 8 minutes. Closing the
CoolBox 30 lid will not further decrease the CoolRack module temperature, however it will extend
the cooling duration.
Temperature Measurement
The CoolRack module thermoconductive design ensures that all well temperatures are identical.
Test equipment
• Temperature probe with small thermal mass such as a K-type beaded thermocouple probe
• Digital thermometer to match probe
• Representative closed sample tube with cap
Test procedure
• Drill a small hole in the center of the sample tube cap with a diameter that provides a snug fit for
the temperature probe wire so there will be minimal ambient air influx into the tub
• Fill the sample tube with 1.0 mL of water
• Place the cap on sample tube and insert temperature probe
• Place the instrumented sample tube in any well of the CoolRack module
• Rack temperatures can be measured by direct contact of the probe in any of the rack wells
-30°C to -20°C
graph A
Fill the CoolBox cavity with pulverized dry ice level with
the bottom of the finger grip recesses as shown. Place
the insulator pad onto the dry ice. Place the CoolRack
on top of the insulator pad. CoolRack temperatures will
quickly equilibrate to approximately -20°C and slowly
drop to -30C as the dry ice sublimates.
Important: Keep lid open for -30º C work. Closing the
CoolBox lid will cause the CoolRack temperature to
drop significantly.
CoolRack PF30,M30,CF30
CoolBox
CoolRack PF30,M30,CF30
CoolBox
A
B
Crushed
Dry Ice
Crushed
Dry Ice
-78°C
graph B
Fill the CoolBox cavity with pulverized dry ice
up to level with the bottom of the finger grip
recesses as shown. Place the CoolRack directly
onto the dry ice. CoolRack temperatures
will quickly reach dry ice temperature in
approximately 8 minutes. Closing the CoolBox
lid will not further decrease the CoolRack
temperature, however it will extend the cooling
duration.
10º C
-20
-33
-80
1
0
2
3
4
5
6 hours
Lid open
Lid open
Caution: The product described here are intended for the exclusive use by trained and experienced laboratory and medi-
cal personnel. A portion of the methods described below require the use of dry ice. Direct skin contact with dry ice or
metal components that have been touching dry ice can cause freezing injury. Always use appropriate protective equipment
for eyes and skin when handling dry ice and cold metal components.
0.5°C to 4°C (Wet Ice Cartridge)
When conventional ice cooling is needed, place the red ice cartridge flat side down in the CoolBox and fill the cartridge with
crushed ice. It is fine to slightly overfill the red cartridge as the CoolRack will settle onto the cartridge quickly during initial cool
down. Place a room temperature or a pre-chilled (4°C) CoolRack on top of the cartridge. Melting ice and ice-water will absorb heat
from the CoolRack through the thermo-conductive ice cartridge and will continue to cool until all ice has melted - up to approxi-
mately 10 hours with lid closed.
Optional Insulator
CoolRack PF30,M30,CF30
CoolBox
-12ºC Cartridge
CoolRack PF30,M30,CF30
CoolBox
Wet Ice Cartridge
-12°C to -4°C
Freeze green -12°C cartridges in a -20°C freezer for a
minimum of 6 hours. Pre-chill the CoolRack in the -20°C
freezer for approximately one-half hour. Place a frozen
cartridge in the CoolBox followed by the pre-chilled
CoolRack.
For cooling beyond the times shown below simply
exchange the cooling cartridge with a recharged one.
Use insulator pad for extended cooling time:
Using the insulator pad on top of the CoolRack or closing
the lid will significantly enhance the useful cooling time.
Tubes can be inserted through the pad.
0ºC
-4
-18
1
0
2
3
5
4
6
7 hours
Lid open
Lid closed
0
4
10º C
1
0
2 3
5
4
6 7 8 9
11 hours
10
Lid open
Lid closed
Use insulator below
CoolRack
* Results may vary when room temperature exceeds 18-20 °C
* Results may vary when room temperature exceeds 18-20 °C
* Results may vary when room temperature exceeds 18-20 °C
*Results may vary when room temperature
exceeds 18-20˚C
*Results may vary when room temperature
exceeds 18-20˚C
CoolRack module
Base
Lid
Base
Pulverized
dry ice
CoolRack module
Lid
Base
Lid
CoolRack module
-20˚C Cartridge
(green)
-20
