XT Freezing Core
Working
Temperature Range
CoolBox XT
Cooling Source
Freezer
Temperature
Temperature Duration
*
Open Lid
Temperature Duration
*
Closed Lid
-20° to 0
o
C
XT Freezing Core
-20
o
C
Over 5 hours
Over 8 hours
-20° to 0
o
C
XT Freezing Core
-80
o
C
Over 8 hours
Over 12 hours
Maintaining samples at -20° to 0°C
Freeze XT Freezing Core in a -20°C freezer for at least 12 hours, or for a faster start, freeze in a -80°C
XT Cooling Core
Working
Temperature Range
Temperature Duration
Open Lid
Temperature Duration
Closed Lid
0.5° to 4.0
o
C
Over 10 hours
Over 16 hours
Maintaining samples at 0.5° to 4°C
Freeze XT Cooling Core in a -20°C freezer for at least 12 hours. XT Cooling Core should be stored
in -20°C freezer when not in use so it is ready when needed. Note: Freezing XT Cooling Core for less
than the specified time will result in decreased cooling duration.
When using a room temperature CoolRack
®
or CoolSink
®
modules
• Remove two XT Cooling Cores from the freezer and place into base.
• Fit magnetized collar onto base, seating it securely.
• Place CoolRack
®
or CoolSink
®
modules directly onto core and allow to equilibrate to 4°C
(approximately 10 -15 minutes).
• Load samples.
• Place lid on CoolBox 2XT when not processing samples to maximize cooling duration.
When using charged XT Cooling Cores and pre-chilled (1°C or less) CoolRack
®
or CoolSink
®
modules
• Remove XT Cooling Cores from the freezer and place on benchtop for approximately 10 minutes.
When the temperature indicator displays 1°C, XT Cooling Core is ready to use.
IMPORTANT: Failure to allow XT Cooling Cores to reach 1°C may result in undesired sample freezing.
• Place XT Cooling Cores into base.
• Fit magnetized collar onto base, seating it securely.
• Place the CoolRack or CoolSink modules of choice onto the core. No equilibration time is needed.
• Load samples.
• Place lid on CoolBox 2XT when not processing samples to maximize cooling duration.
Assembly of CoolBox
™
2XT cont.
1. Remove XT Cooling Cores from the freezer and place
on benchtop for approximately 10-15 minutes. When
the temperature indicator displays 1°C, XT Cooling
Core is ready to use. Note: Frost will form on the core
exterior upon removal from the freezer; when the frost
liquifies, the core is at proper temperature.
2. Place two XT Cooling Cores into CoolBox 2XT base.
3. Fit magnetized collar onto CoolBox 2XT base, seating
it securely.
4. Place two CoolRack
®
or CoolSink
®
modules of choice
onto XT Cooling Cores.
5. Load samples.
6. Place lid on CoolBox 2XT when not processing samples
to maximize cooling duration.
Lid
Magnetized collar
XT Cooling Core(s)
or XT Freezing
Core(s)(Pre-frozen in
-20˚C freezer)
Magnetized base
freezer for at least 6 hours. Freezing in a -80°C freezer also prolongs cooling duration. XT Freezing
Core should be stored in a -20°C or -80°C freezer when not in use so it is ready when needed. Note:
Freezing XT Freezing Core for less than the specified time will result in decreased cooling duration.
When using a room temperature CoolRack
®
or CoolSink
®
module
• Remove XT Freezing Core from the freezer and place into base.
• Fit magnetized collar onto the base, seating it securely.
• Place the room temperature CoolRack or CoolSink module of choice onto core and allow to
equilibrate to 0°C (approximately 10 minutes).
• Load samples.
• Place lid on CoolBox 2XT when not processing samples to maximize cooling duration.
When using a pre-chilled (1°C or less) CoolRack
®
or CoolSink
®
module
• Remove XT Freezing Core from the freezer and place into base.
• Fit magnetized collar onto the base, seating it securely.
• Place the CoolRack or CoolSink module of choice onto core.
• Load samples.
• Place lid on CoolBox 2XT when not processing samples to maximize cooling duration.
Dry Ice
Working
Temperature Range
CoolBox XT
Cooling Source
Temperature Duration
*
Open Lid
Temperature Duration
*
Closed Lid
-78
o
C
200ml dry ice
Over 4 hours
Over 5 hours
Maintaining or snap-freezing samples at -78°C
• Remove XT Cooling or Freezing Cores from the CoolBox
™
2XT base.
• Fill the base, with approximately 200ml of pulverized dry ice.
• Fit magnetized collar onto the base, seating it securely.
• Place the CoolRack or CoolSink modules directly onto dry ice and allow module to equilibrate to
-78°C (approximately 7-8 minutes).
• Load samples.
• If snap-freezing, freezing will occur in 7-10 minutes depending upon sample volume and type.
Note: The thermo-conductive design of CoolRack
®
and CoolSink
®
modules ensures uniform well-to- well
temperature regardless of the consistency of the dry ice.
Care and Cleaning
The CoolBox
™
2XT housing is constructed from a cross-linked, closed-cell polyethylene foam. The
material has excellent resistance to fluid absorption and abrasion. Do not use the CoolBox XT base
for pulverizing dry ice. Maximum temperature exposure: 60°C. Avoid prolonged exposure to UV
light sources.
All components including housing, XT Cooling Core and optional XT Freezing Core are compatible
with repeated and prolonged cryogenic temperature exposure. All components can be cleaned
with aqueous detergents, alcohol, 10% bleach, and acid/base viricide (such as Virkon S) solutions.
Rinse with clear water after using cleaning solutions. Do not autoclave. CoolRack
®
and CoolSink
®
sample modules may be autoclaved, or cleaned with alcohol or 10% bleach.
Dimensions (L x W x H)
Interior (with XT Cooling Core in base): 21.0 x 14.3 x 13.4 cm / 8.3 x 5.6 x 5.3 in
Exterior: 26.5 x 20.0 x 15.0 cm / 10.4 x 7.9 x 6.3 in
