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BRIGHT OTF 5000, Instruction Manual

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BRIGHT OTF 5000 Instruction Manual Download Page 6

OTF 5000/HS/LS-001/2/3/4 Iss 4 June 2012  

iv 

7

 

PRODUCT INFORMATION 

41

 

SPECIAL FEATURES: 

41

 

Configurations 

41

 

Basic Specification 

41

 

LS Specification 

42

 

HS Specification 

42

 

 

Appendices 

 
5040 MICROTOME DRAWINGS (relevant to Model) 
 
5040 MICROTOME PART LIST (relevant to Model) 
 
ELECTRICAL CIRCUIT DIAGRAMS (relevant to Model) 
 
Material Safety Sheet: CRYOSPRAY 57713 
 
Material Safety Sheet: CRYO-M-BED 53581 
 
Material Safety Sheet: LOW TEMPERATURE OIL 57491 
 
DECONTAMINATION CERTIFICATE 
 

Summary of Contents for OTF 5000

Page 1: ...strial Estate Huntingdon Cambridgeshire England PE29 6EU Tel 44 0 1480 454528 451980 451499 Fax 44 0 1480 456031 Email sales brightinstruments com Website www brightinstruments com INSTRUCTION MANUAL...

Page 2: ......

Page 3: ...STALLATION OF TRAYS AND SHELVES FIG 2 4 1 2 4 FROST ELIMINATION COVER FIG 3 5 1 2 5 INSTALLATION OF THE MICROTOME and REMOVAL 5 1 2 6 INSULATED NIGHT PLUG 6 1 2 7 X THIN SECTION CONVERSION KIT where a...

Page 4: ...E BLOCK 16 2 2 1 KNIFE GUARDS 16 2 2 2 KNIFE HOLDER 17 2 2 3 X THIN SECTION CONVERSION KIT where applicable 17 2 2 4 ANTI ROLL ASSEMBLY 18 2 3 MICROTOME CONTROLS Quick Release Feather Blade Holder 20...

Page 5: ...USES 33 4 7 2 CHANGING FLUORESCENT TUBE 33 4 8 DECONTAMINATION 33 5 ACCESSORIES 34 5 1 OBJECT HOLDERS 34 5 2 KNIVES 36 5 3 LEVER RELEASE FEATHER BLADE HOLDER 37 5 4 MAGNAPLATE ANTI ROLL PLATE 37 5 5 M...

Page 6: ...Specification 42 HS Specification 42 Appendices 5040 MICROTOME DRAWINGS relevant to Model 5040 MICROTOME PART LIST relevant to Model ELECTRICAL CIRCUIT DIAGRAMS relevant to Model Material Safety Shee...

Page 7: ...results b Fuses Fuse ratings are clearly indicated on all fuse panels adjacent to the fuse holder If and when replacement is necessary the correct fuse rating must be adhered to c Earthing Grounding...

Page 8: ...t of it is to be returned to Bright Instrument Co Ltd please note the following 1 If the instrument or any part of it has been exposed to or been in contact with potential pathogenic or radioactive ma...

Page 9: ...gence or otherwise in respect of defects in goods delivered or for any injury damage or loss resulting from such defects and the Seller s liability under this clause shall be in lieu of any warranty o...

Page 10: ...ress of moist laboratory air The drain plug should be left in position except when cleaning is carried out 1 2 3 INSTALLATION OF TRAYS AND SHELVES FIG 2 A pair of stainless steel trays is packed separ...

Page 11: ...icrotome to locate the front two studs the rear ones will follow 3 Loosen the two recessed grub screws in the coupling on the Drive shaft projecting from the right chamber wall 4 Slide the coupling on...

Page 12: ...2 Remove Advance Rewind Knob on top of microtome by pulling upward 3 Remove LH tray by sliding forward and up 4 If a heated v block is fitted then lift it move it out of the way 5 Remove Coupling by l...

Page 13: ...st eight hours and preferably overnight before switching on Moving the instrument around on its castors e g from one laboratory to another will not affect the compressor oil 1 3 2 ELECTRICAL REQUIREME...

Page 14: ...with long lasting temperature stability the OTF5000 can truly be described as complete FIG 1 SWITCHING ON On receipt of your new Bright instrument please refer to the Receipt and Unpacking section and...

Page 15: ...clear the evaporator cooling fins without the rest of the chamber rising above 0 C Set the 12 hour analogue clock to the current time by turning the clear plastic disc with your finger Note that beca...

Page 16: ...than the set chamber temperature or if the chamber temperature goes 20 C colder than the set temperature The Alarm can be manually reset by pressing any one of the 3 Controller buttons on the Chamber...

Page 17: ...ination cover after using the quick freezer plate this helps prevent a build up of frost 2 1 8 QF SPECIMEN QUICK FREEZER where applicable FIG 10 The specimen quick freezer QF is designed for rapid fre...

Page 18: ...0 C If the specimen is then placed in the vice jaws it will be far too cold to cut and if attempted damage may occur to knives It is imperative that a little time is allowed for the specimen temperatu...

Page 19: ...andwheel The longer the switch is pressed the thicker the section If the Advance switch is pressed for more than 1 5 seconds a faster advance of the specimen is achieved Motorised Specimen Rewind 1 To...

Page 20: ...ions can be used at any time Pull out the connecting rod knob to operate the microtome manually The Emergency Stop button stays in when pressed Twist button in direction of arrows to release Then pres...

Page 21: ...ain active To prevent airborne contamination in the chamber due to convection air currents caused by the refrigeration system it is recommended that the UV system is switched on at all times the cryos...

Page 22: ...e guard 19 Anti roll extension knob 10 Knife guard release knob 2 2 1 KNIFE GUARDS A pair of knife guards 9 are fitted and it is strongly recommended that they remain fitted at all times When access t...

Page 23: ...guards to the centre and slide the knife out through to the left 2 2 3 X THIN SECTION CONVERSION KIT where applicable This kit consists of a 0 2 12 m feed screw part number 50256 an orientating glass...

Page 24: ...ion 3 2 3 Release the knife block clamp locking lever 3 and turn the knife carriage feed control 4 clockwise to bring the knife towards the specimen 4 Trim the specimen or embedding medium until a sui...

Page 25: ...to cut sections again They will curl up over the roll plate 5 Slowly turn the anti roll micro adjusting knob 17 clockwise while cutting sections When the plate 15 reaches the correct height the secti...

Page 26: ...Roll Plate Adjuster 3 Blade Angle Indicator 11 Stem Clamp Knob 4 Wire Knife Guard 12 Blade 5 Blade Loading Plate 13 Anti Roll Plate Clamp Knob 6 Fixed Blade Guard 14 T Piece Clamp Knob 7 Easi Set Ant...

Page 27: ...towards it To install a blade Feather blades are covered with a fine film of protecting varnish It is necessary to remove this varnish using solvents i e xylene or acetone before using a blade for sec...

Page 28: ...leading edge of the anti roll plate is parallel to the edge of the blade b the leading edge of the anti roll plate is parallel in line with the blade c the under side of the anti roll plate is at an a...

Page 29: ...ed control 2 4 2 SECTION THICKNESS CONTROL See above To select section thickness set the thickness control 1 at zero and rotate anticlockwise until desired thickness is level with the indicator To res...

Page 30: ...AR 50258 manual 50258 1 MAR 50259 manual 50259 1 MAR 50260 manual Thin Section Standard in cryostat Standard in wax microtome 0 2 to 12 m 0 5 to 30 m 1 to 60 m 2 to 120 m 3 to 180 m 5 to 300 m 0 2 m i...

Page 31: ...l rule the quicker tissues are frozen the better the results will be The aim is to minimise the damage caused by ice crystal artefact and the more rapid the transition from liquid phase to solid phase...

Page 32: ...freezing media 3 1 5 BRIGHT CLINI RF The Bright Clini RF is a small free standing top opening ultra low temperature freezer Its function is to rapidly freeze tissue specimens ready for cryotomy or for...

Page 33: ...der from the quick freezer and place it in the V block or cryomatic block and tighten the screw firmly to hold it in position Wait a few minutes for temperatures to equalise before trimming It is ofte...

Page 34: ...t sections if adjustments to the anti roll plate are required see section 2 2 8 4 To collect sections have a clean microscope slide ready at ambient temperature Bring the slide towards the knife swing...

Page 35: ...of factors The notes which follow deal with the more commonly encountered difficulties and suggest a systematic approach to problem solving 3 6 1 SOLUTIONS CHECK LIST First check all the basic factor...

Page 36: ...d debris always brush up the knife never down or along Sections curl over anti roll plate anti roll plate too low Section curl under anti roll plate anti roll plate at too large an angle to knife Sect...

Page 37: ...tion 2 2 2 and clean it see section 4 4 4 Remove the microtome from the chamber the reverse of the installation procedure see section 1 2 5 Remove drain plug from base of chamber 5 Place the microtome...

Page 38: ...until the arrival of the engineer Failure to carry out this action will result in the service visit being cancelled and could incur further call out charges A completed decontamination certificate mus...

Page 39: ...use holder 6 Reconnect the power supply and ensure instrument is working correctly If there are any further problems contact your local distributor or Bright Instrument Co Ltd direct 4 7 2 CHANGING FL...

Page 40: ...ct holder 22mm long type gives greater clearance between specimen clamp and knife holder Ideal for when multi position glass slides are used 50735 1 Object holder with stem 37mm dia Suitable for large...

Page 41: ...ect holder discs 50mm dia pack of 100 Orientating specimen holders 51695 Universal holder This simple inexpensive device will fit 5030 or 5040 microtomes that have fixed heads Any of the object holder...

Page 42: ...older for Shandon knife sharpener safety cut outs at each end 50232 1 Hardened steel knife supplied in box C profile 160 x 35 x 9mm 15 angle suitable for large area sections 50207 Similar to 50232 1 b...

Page 43: ...will Fit most knives and blades It is shown sitting on a 50230 standard knife The Magnaplate is supplied in a pack of 3 with a single removable handle 5 5 MAGNACUT MAGNETIC DISPOSABLE BLADE SYSTEM 50...

Page 44: ...SCREWS 5030 and 5040 microtomes are fitted with a feed screw that controls the section thickness range These feed screws may be freely changed so that a new section thickness range can be given to th...

Page 45: ...6 bottles Bright Cryo M Bed is a blend of polyviol alcohol 217 thymol and water It is an inert colourless viscous fluid which is totally transparent at room temperature It becomes opaque when frozen a...

Page 46: ...fe with box 228 x 38 x 6mm Suitable for cutting bone and hard plastics NB needs to be resharpened at our factory 52647 Stem for 50232 1 and 50234 knives 52671 Knife sharpening kit Includes hone strop...

Page 47: ...defrost supplied as standard Non urgent functions on side panel Long list of options and accessories Contains tried and tested 5040 rotary microtome Configurations The OTF5000 cryostats are available...

Page 48: ...gotiate doorways a further 65mm can be gained LS Specification As the basic specification but with the addition of the following items Object orientation Fine with 8 in horizontal and vertical axes 36...

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