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Pipetting
Wait: 1-2 seconds for 2–300 μL volumes, 2-3 seconds for
1000 μL (longer for viscous solutions).
2. Still holding the plunger, withdraw the tip, sliding it along
the wall of the vessel. Release the plunger.
3. Press the tip ejector button to discard the tip. Use a new
tip for each sample to prevent carry-over. Repeat for the
next pipetting cycle.
Good Pipetting Guidelines
xPIPETTE incorporates features to deliver pipetting
consistency. You should also maintain:
1. Consistent pickup and dispense rhythm.
2. Consistent speed and smoothness when pipetting.
3. Consistent pressure on the plunger button at the first
stop.
4. Consistent immersion depth.
5. Pipette vertically, or within 20° of vertical.
6. Don’t invert or lay the pipette flat with liquid in the tip.
Pre-Rinsing Recommended
Some solutions may leave a film on the inside tip wall. This
film remains relatively constant in successive pipetting with
the same tip, so excellent precision can be obtained by
refilling the tip and using the refilled volume as the sample.
Successive samples from this same tip will exhibit good
reproducibility.
Temperature Considerations
Warm or cold liquids can be measured with good preci-
sion by using a consistent pipetting rhythm. This will help
minimize any differences in heating or cooling effects within
the pipette. Use a new pipette tip each time for optimal
accuracy and precision when measuring samples with
temperatures greatly different from ambient, and do not
pre-rinse. As with any air-displacement pipette, you will get
the best results if there is no delay between aspirating the
sample and dispensing it.
Volume Range (μL)
Immersion Depth
(mm)
Wait Time
(sec)
0.1-10
1-2
1
10-200
2-3
1
200-1000
3-6
2-3
xPIPETTE Manual
Summary of Contents for 63300164
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